Abstract
Rapid clonal propagation through in vitro techniques using cotyledonary node as an explant has been attempted in the high yielding, tikka-susceptible (ICG 11337, AK 1224, ICGS 44, JL 24) and tikka-immune (ICG 6284) varieties of Arachis hypogaea L. Cotyledonary nodes were excised from the 12–15 days-old axenic seedlings and cultured in the presence of various concentrations of N 6 –benzylaminopurine (BAP) (1, 5, 10, 15, 25 and 50 mg L –1 ). BAP-free control showed very little or no sign of multiplication in terms of multiple shoot, axillary branch and shoot bud formation. In the presence of higher concentrations of BAP, cultured explants showed the development of multiple shoots, axillary branches and shoot buds. Thus, a tri-directional multiplication pathway (multiple shoot, axillary branch and shoot bud) in a single medium has been achieved in the tested varieties of Arachis hypogaea L. The regeneration of maximum number of shoots, axillary branches and shoot buds from the excised cotyledonary nodes and their corresponding requirements for BAP differed across varieties, which might be due to variation at their genotypic levels. The isolated shoots were quickly rooted in the presence of a-naphthalene acetic acid (NAA) (1 mg L –1 ) and well-rooted plantlets were successfully transferred
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Claims 7
Claim · model-written summary
BAP-free controls showed little or no multiplication.
- Taxon
- Arachis hypogaea L.
Arachis hypogaeat0 - Treatment
- BAP-free controle0
- Outcome
- multiplication in terms of multiple shoot, axillary branch and shoot bud formatione0
- Direction
- very little or no signe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BAP-free control showed very little or no sign of multiplication in terms of multiple shoot, axillary branch and shoot bud formation.”
t0“Arachis hypogaea L.”
Claim · model-written summary
Higher BAP concentrations produced multiple shoots, axillary branches and shoot buds.
- Taxon
- Arachis hypogaea L.Arachis hypogaeat0
- Treatment
- higher concentrations of BAPe0
- Outcome
- development of multiple shoots, axillary branches and shoot budse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the presence of higher concentrations of BAP, cultured explants showed the development of multiple shoots, axillary branches and shoot buds.”
t0“Arachis hypogaea L.”
Claim · model-written summary
A single medium achieved three routes of multiplication across tested varieties.
- Taxon
- Arachis hypogaea L.Arachis hypogaeae0
- Outcome
- tri-directional multiplication pathwaye0
- Value
- tri-directionale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thus, a tri-directional multiplication pathway (multiple shoot, axillary branch and shoot bud) in a single medium has been achieved in the tested varieties of Arachis hypogaea L.”
Claim · model-written summary
Maximum shoot, branch and bud regeneration differed across varieties.
- Taxon
- Arachis hypogaea L.Arachis hypogaeat0
- Material
- varietiese0
- Outcome
- regeneration of maximum number of shoots, axillary branches and shoot budse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regeneration of maximum number of shoots, axillary branches and shoot buds from the excised cotyledonary nodes and their corresponding requirements for BAP differed across varieties”
t0“Arachis hypogaea L.”
Claim · model-written summary
BAP requirements differed across varieties.
- Taxon
- Arachis hypogaea L.Arachis hypogaeat0
- Material
- varietiese0
- Outcome
- requirements for BAPe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their corresponding requirements for BAP differed across varieties”
t0“Arachis hypogaea L.”
Claim · model-written summary
NAA rooted isolated shoots quickly.
- Taxon
- Arachis hypogaea L.Arachis hypogaeat0
- Treatment
- a-naphthalene acetic acid (NAA) (1 mg L –1 )e0
- Outcome
- rootede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The isolated shoots were quickly rooted in the presence of a-naphthalene acetic acid (NAA) (1 mg L –1 )”
t0“Arachis hypogaea L.”
Claim · model-written summary
Well-rooted plantlets were successfully transferred.
- Taxon
- Arachis hypogaea L.Arachis hypogaeat0
- Outcome
- successfully transferrede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“well-rooted plantlets were successfully transferred”
t0“Arachis hypogaea L.”
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