Abstract
The efficient plantlets regeneration of peanut is the most important and a primary step to successfully transform gene and apply recently developed genome editing techniques for crop yield improvement. The purpose of this research is to develop protocol for peanut cultivars applying different concentration of hormones for selected peanut cultivars and develop plantlets regeneration protocol. There is no previously reported protocol for the Chinese peanut cultivar N3 and Yu-hua-14. We optimized shoot and root regeneration protocol for two peanut cultivars. Both cultivars showed positive response for the cytokinin plant growth hormone 6-benzylaminopurine (BAP) and thidiazuron (TDZ). The highest shooting rate (97%) was found in a medium supplemented with 4 mg/L BAP and (94.33%) for 1 mg/L TDZ. Hence, more shoot initiation was observed at higher concentration of BAP as compared to TDZ. However, the maximum root regeneration (81%) was found on medium containing 0.3 mg/L 2, 4-dichlorophenoxyacetic acid (2,4-D) and the highest rooting rate (96.33%) was found in a medium containing 1 mg/L -naphthalene acetic acid (NAA), indicating lower concentration of NAA induce more rooting rate than 2,4-D treatment. In this study, cotyledonary node was used, and this method was found to be efficient and rapid for in vitro peanut regeneration.
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Claims 7
Claim · model-written summary
The highest shooting rate in peanut cotyledonary nodes (97%) was found on medium with 4 mg/L BAP.
- Taxon
- Arachis hypogaea
Arachis hypogaeat0 - Organ / stage
- cotyledonary nodee2
- Treatment
- 4 mg/L BAPe1
- Outcome
- shooting ratee1
- Direction
- higheste1
- Value
- 97%e1
- Setting
- in vitroe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arachis hypogaea”
e1“The highest shooting rate (97%) was found in a medium supplemented with 4 mg/L BAP and (94.33%) for 1 mg/L TDZ”
e2“cotyledonary node was used”
e3“this method was found to be efficient and rapid for in vitro peanut regeneration”
Claim · model-written summary
Shooting rate of 94.33% was reached on medium with 1 mg/L TDZ in peanut.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Organ / stage
- cotyledonary nodee2
- Treatment
- 1 mg/L TDZe1
- Outcome
- shooting ratee1
- Value
- 94.33%e1
- Setting
- in vitroe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arachis hypogaea”
e1“The highest shooting rate (97%) was found in a medium supplemented with 4 mg/L BAP and (94.33%) for 1 mg/L TDZ”
e2“cotyledonary node was used”
e3“this method was found to be efficient and rapid for in vitro peanut regeneration”
Claim · model-written summary
More shoot initiation was observed at a higher BAP concentration than with TDZ in peanut.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Treatment
- higher concentration of BAPe4
- Outcome
- shoot initiatione4
- Direction
- moree4
- Comparator
- TDZe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arachis hypogaea”
e4“Hence, more shoot initiation was observed at higher concentration of BAP as compared to TDZ”
Claim · model-written summary
The maximum root regeneration (81%) in peanut was found on medium with 0.3 mg/L 2,4-D.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Treatment
- 0.3 mg/L 2, 4-dichlorophenoxyacetic acid (2,4-D)e5
- Outcome
- root regeneratione5
- Direction
- maximume5
- Value
- 81%e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arachis hypogaea”
e5“the maximum root regeneration (81%) was found on medium containing 0.3 mg/L 2, 4-dichlorophenoxyacetic acid (2,4-D)”
Claim · model-written summary
The highest rooting rate (96.33%) in peanut was found on medium with 1 mg/L NAA.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Treatment
- 1 mg/L -naphthalene acetic acid (NAA)e6
- Outcome
- rooting ratee6
- Direction
- higheste6
- Value
- 96.33%e6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arachis hypogaea”
e6“the highest rooting rate (96.33%) was found in a medium containing 1 mg/L -naphthalene acetic acid (NAA)”
Claim · model-written summary
Lower NAA concentration gave a higher rooting rate than 2,4-D in peanut.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Treatment
- lower concentration of NAAe7
- Outcome
- rooting ratee7
- Direction
- moree7
- Comparator
- 2,4-D treatmente7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arachis hypogaea”
e7“indicating lower concentration of NAA induce more rooting rate than 2,4-D treatment”
Claim · model-written summary
Both peanut cultivars responded positively to cytokinins BAP and TDZ.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Treatment
- cytokinin plant growth hormone 6-benzylaminopurine (BAP) and thidiazuron (TDZ)e0
- Outcome
- positive responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arachis hypogaea”
e0“Both cultivars showed positive response for the cytokinin plant growth hormone 6-benzylaminopurine (BAP) and thidiazuron (TDZ)”
The same questions in other papers
1-naphthaleneacetic acid · growth · Arachis hypogaea (peanut)Results differ
19 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
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See all 22 papers →acetic acid · growth · Arachis hypogaea (peanut)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
2,4-D · growth · Arachis hypogaea (peanut)Positive and negative results
11 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.