Abstract
Agrobacterium vitis nontumorigenic strain F2/5 is able to inhibit crown gall disease on grapevines. The mechanism of grape tumor inhibition (GTI) by F2/5 has not been fully determined. In this study, we demonstrate that two nonribosomal peptide synthetase (NRPS) genes (F-avi3342 and F-avi5730) and one polyketide synthase gene (F-avi4330) are required for GTI. Knockout of any one of them resulted in F/25 losing GTI capacity. We previously reported that F-avi3342 and F-avi4330 but not F-avi5730 are required for induction of grape tissue necrosis and tobacco hypersensitive response. F-avi5730 is predicted to encode a single modular NRPS. It is located in a cluster that is homologous to the siderophore vicibactin biosynthesis locus in Rhizobium species. Individual disruption of F-avi5730 and two immediate downstream genes, F-avi5731 and F-avi5732, all resulted in reduced siderophore production; however, only F-avi5730 was found to be required for GTI. Complemented F-avi5730 mutant (ΔF-avi5730(+)) restored a wild-type level of GTI activity. It was determined that, over time, populations of ΔF-avi4330, ΔF-avi3342, and ΔF-avi5730 at inoculated wound sites on grapevine did not differ from those of ΔF-avi5730(+) indicating that loss of GTI was not due to reduced colonization of wound sites by mutants.
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Claims 8
Claim · model-written summary
F-avi3342 is required for grape tumor inhibition.
- Interactor
- Agrobacterium vitisGillisella vitise1biocontrol_agent
- Gene
- F-avi3342e0
- Outcome
- GTIe0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two nonribosomal peptide synthetase (NRPS) genes (F-avi3342 and F-avi5730) and one polyketide synthase gene (F-avi4330) are required for GTI”
e1“Agrobacterium vitis nontumorigenic strain F2/5 is able to inhibit crown gall disease on grapevines”
Claim · model-written summary
F-avi5730 is required for grape tumor inhibition.
- Interactor
- Agrobacterium vitisGillisella vitise1biocontrol_agent
- Gene
- F-avi5730e0
- Outcome
- GTIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two nonribosomal peptide synthetase (NRPS) genes (F-avi3342 and F-avi5730) and one polyketide synthase gene (F-avi4330) are required for GTI”
e1“Agrobacterium vitis nontumorigenic strain F2/5 is able to inhibit crown gall disease on grapevines”
Claim · model-written summary
F-avi4330 is required for grape tumor inhibition.
- Interactor
- Agrobacterium vitisGillisella vitise1biocontrol_agent
- Gene
- F-avi4330e0
- Outcome
- GTIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two nonribosomal peptide synthetase (NRPS) genes (F-avi3342 and F-avi5730) and one polyketide synthase gene (F-avi4330) are required for GTI”
e1“Agrobacterium vitis nontumorigenic strain F2/5 is able to inhibit crown gall disease on grapevines”
Claim · model-written summary
Disrupting F-avi5730 reduced siderophore production.
- Treatment
- disruption of F-avi5730e0
- Interactor
- Agrobacterium vitisGillisella vitise2biocontrol_agent
- Gene
- F-avi5730e0
- Outcome
- siderophore productione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Individual disruption of F-avi5730 and two immediate downstream genes, F-avi5731 and F-avi5732, all resulted in reduced siderophore production”
e1“wound sites on grapevine”
e2“Agrobacterium vitis nontumorigenic strain F2/5”
Claim · model-written summary
Disrupting F-avi5731 reduced siderophore production.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Individual disruption of F-avi5730 and two immediate downstream genes, F-avi5731 and F-avi5732, all resulted in reduced siderophore production”
e1“wound sites on grapevine”
Claim · model-written summary
Disrupting F-avi5732 reduced siderophore production.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Individual disruption of F-avi5730 and two immediate downstream genes, F-avi5731 and F-avi5732, all resulted in reduced siderophore production”
e1“wound sites on grapevine”
Claim · model-written summary
Complementing the F-avi5730 mutant restored wild-type GTI activity.
- Treatment
- Complemented F-avi5730 mutant (ΔF-avi5730(+))e0
- Gene
- F-avi5730e0
- Outcome
- GTI activitye0
- Direction
- restorede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Complemented F-avi5730 mutant (ΔF-avi5730(+)) restored a wild-type level of GTI activity”
e1“wound sites on grapevine”
Claim · model-written summary
Mutants did not differ in wound-site populations from the complemented F-avi5730 strain.
- Outcome
- populations of ΔF-avi4330, ΔF-avi3342, and ΔF-avi5730 at inoculated wound sitese0
- Direction
- did not differe0
- Comparator
- ΔF-avi5730(+)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“populations of ΔF-avi4330, ΔF-avi3342, and ΔF-avi5730 at inoculated wound sites on grapevine did not differ from those of ΔF-avi5730(+)”