Paper · 2011 · Indian Journal of Plant Genetic Resources

Seed Storage Protein Profi les of Pea (Pisum sativum L.) Genotypes using SDS-PAGE

Deepali Pandey; YV Singh

7
claims
14
verbatim quotes
0
citations
2
research areas
✓
Open access

Abstract

Using polyacrylamide slab gel electrophoresis, the water soluble seed proteins could be resolved in 42 bands distributed in six zones. One hundred and thirteen cultivars were categorized into 28 groups; each group had distinct and different electropherotic banding patterns. However, within the group cultivars were also further distinguishable by the intensity of staining of bands. It was possible through electrophoresis to differentiate phenotypically similar cultivars for example UU-12, Multifreezer, Piourette, Starcovert, Bonneville and IP-3. Cultivar Meethi Phali with a specific characteristic of edible pod type formed a separate group with 9 bands. Most of the varieties showed no distinct patterns which would allow their characterization. There were a few specific bands in each variety and most of them; although exclusive, appeared at such a low frequency that varietal characterization was not possible.

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Claims 7

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Electrophoresis resolved water-soluble seed proteins into 42 bands in six zones.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Outcome
water soluble seed proteinse0
Value
42 bandse0
Qualifier
six zonese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Using polyacrylamide slab gel electrophoresis, the water soluble seed proteins could be resolved in 42 bands distributed in six zones.”

✓ located verbatim in the source · abstract, characters 0–134 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 39–55 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

113 cultivars were classified into 28 groups with distinct banding patterns.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Outcome
distinct and different electropherotic banding patternse0
Value
28 groupse0
Qualifier
One hundred and thirteen cultivarse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“One hundred and thirteen cultivars were categorized into 28 groups; each group had distinct and different electropherotic banding patterns.”

✓ located verbatim in the source · abstract, characters 135–274 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 39–55 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Cultivars within groups could be distinguished by staining intensity.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Outcome
further distinguishable by the intensity of staining of bandse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“However, within the group cultivars were also further distinguishable by the intensity of staining of bands.”

✓ located verbatim in the source · abstract, characters 275–383 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 39–55 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Electrophoresis differentiated phenotypically similar cultivars.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Outcome
differentiate phenotypically similar cultivarse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“It was possible through electrophoresis to differentiate phenotypically similar cultivars for example UU-12, Multifreezer, Piourette, Starcovert, Bonneville and IP-3.”

✓ located verbatim in the source · abstract, characters 384–550 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 39–55 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Edible-pod cultivar Meethi Phali formed a separate group with nine bands.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Material
Meethi Phalie0
Outcome
formed a separate groupe0
Value
9 bandse0
Qualifier
edible pod typee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Cultivar Meethi Phali with a specific characteristic of edible pod type formed a separate group with 9 bands.”

✓ located verbatim in the source · abstract, characters 551–660 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 39–55 · show in text ↑
Method / resourceobservedoriginal resultnegativemeasured

Claim · model-written summary

Most varieties lacked distinct patterns allowing characterization.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Outcome
no distinct patterns which would allow their characterizatione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Most of the varieties showed no distinct patterns which would allow their characterization.”

✓ located verbatim in the source · abstract, characters 661–752 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 39–55 · show in text ↑
Method / resourceobservedoriginal resultnegativemeasured

Claim · model-written summary

Each variety had a few specific bands, but most exclusive bands were too infrequent for varietal characterization.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Outcome
varietal characterization was not possiblee0
Qualifier
a few specific bands in each varietye0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“There were a few specific bands in each variety and most of them; although exclusive, appeared at such a low frequency that varietal characterization was not possible.”

✓ located verbatim in the source · abstract, characters 753–920 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 39–55 · show in text ↑