Abstract
The variation demonstrated by regeneratIng 119 lettuce plants from mesophyll protoplasts of a single variety is described. Twenty regenerated plants had severely reduced fertility. Cytological analysis revealed that, for twelve of these, the reduced fertility probably is due to tetraploidly. Seed arising from self-pollinated, regenerated plants were significantly more variable in size than controls. Plants derived from these seed were examined for somaclonal variation, which occurred in two distinct patterns. Seedlings from 10 protoplast-derived clones segregated for mutant phenotypes in approximately 3:1 ratios suggesting single-gene recessive mutations. One additional clone family segregated for a mutant phenotype at maturity. Seedlings from two other clones were 100 percent variant. No obvious variation was seen in the remaining 58 clonal families that were subjected to field trials. One potentially useful variant was identified that appeared to have increased seedling vigor. The Implications of these findings with respect to the nature and usefulness of somaclonal variation are discussed.
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Claims 9
Claim · model-written summary
Twenty regenerated lettuce plants had severely reduced fertility.
- Taxon
- lettuce
Lactuca sativat0 - Outcome
- fertilitye0
- Direction
- severely reducede0
- Value
- Twentye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twenty regenerated plants had severely reduced fertility”
t0“lettuce plants”
Claim · model-written summary
Tetraploidy probably explained reduced fertility in twelve regenerated plants.
- Taxon
- lettuceLactuca sativat0
- Outcome
- reduced fertilitye0
- Value
- twelvee0
- Qualifier
- tetraploidlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“for twelve of these, the reduced fertility probably is due to tetraploidly”
t0“lettuce plants”
Claim · model-written summary
Self-pollinated regenerated lettuce plants produced significantly more variable seed sizes than controls.
- Taxon
- lettuceLactuca sativat0
- Organ / stage
- Seede0
- Outcome
- variable in sizee0
- Direction
- more variablee0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seed arising from self-pollinated, regenerated plants were significantly more variable in size than controls”
t0“lettuce plants”
Claim · model-written summary
Seedlings from ten protoplast-derived clones segregated mutant phenotypes in about 3:1 ratios.
- Taxon
- lettuceLactuca sativat0
- Material
- protoplast-derived clonese0
- Organ / stage
- Seedlingse0
- Outcome
- mutant phenotypese0
- Value
- approximately 3:1 ratiose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seedlings from 10 protoplast-derived clones segregated for mutant phenotypes in approximately 3:1 ratios”
t0“lettuce plants”
Claim · model-written summary
The segregation suggests single-gene recessive mutations.
- Taxon
- lettuceLactuca sativat0
- Outcome
- mutant phenotypese1
- Qualifier
- single-gene recessive mutationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting single-gene recessive mutations”
e1“Seedlings from 10 protoplast-derived clones segregated for mutant phenotypes in approximately 3:1 ratios”
t0“lettuce plants”
Claim · model-written summary
One additional clone family segregated for a mutant phenotype at maturity.
- Taxon
- lettuceLactuca sativat0
- Material
- clone familye0
- Outcome
- mutant phenotype at maturitye0
- Value
- Onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One additional clone family segregated for a mutant phenotype at maturity”
t0“lettuce plants”
Claim · model-written summary
Seedlings from two other clones were all variant.
- Taxon
- lettuceLactuca sativat0
- Material
- two other clonese0
- Outcome
- variante0
- Value
- 100 percente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seedlings from two other clones were 100 percent variant”
t0“lettuce plants”
Claim · model-written summary
No obvious variation appeared in 58 remaining clonal families in field trials.
- Taxon
- lettuceLactuca sativat0
- Material
- clonal familiese0
- Outcome
- variatione0
- Direction
- No obvious variatione0
- Value
- 58e0
- Setting
- field trialse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No obvious variation was seen in the remaining 58 clonal families that were subjected to field trials”
t0“lettuce plants”
Claim · model-written summary
One potentially useful variant appeared to have increased seedling vigor.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One potentially useful variant was identified that appeared to have increased seedling vigor”
t0“lettuce plants”