Abstract
Shoot tip explants derived from 15-day old aseptically grown seedlings of the hybrid squash were induced to produce multiple shoots on Murashige and Skoog's medium. Higher number of shoots per explant was achieved when MS medium was supplemented with BAP in combination with NAA. Further increase in shoot number was obtained when casein hydrolysate (CH) was added. In respect of medium quality rating, 1 mgl−1 BAP + 0.1 mgl−1 NAA gave a good shoot growth with higher frequency of shooting and greater number of shoots per explant. Regenerated shoots were rooted well in half strength MS medium with IBA and NAA (0.1 mgl−1 each).
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Claims 6
Claim · model-written summary
BAP combined with NAA in MS medium gave a higher number of shoots per explant than MS medium.
Verbatim
e2“Higher number of shoots per explant was achieved when MS medium was supplemented with BAP in combination with NAA”
e1“Shoot tip explants derived from 15-day old aseptically grown seedlings of the hybrid squash”
Claim · model-written summary
Adding casein hydrolysate further increased shoot number.
- Taxon
- hybrid squashe1
- Treatment
- casein hydrolysate (CH)e3
- Outcome
- shoot numbere3
- Direction
- Further increasee3
Not reportedComparator
Verbatim
e3“Further increase in shoot number was obtained when casein hydrolysate (CH) was added”
e1“Shoot tip explants derived from 15-day old aseptically grown seedlings of the hybrid squash”
Claim · model-written summary
1 mg/l BAP with 0.1 mg/l NAA gave higher shooting frequency and more shoots per explant.
- Taxon
- hybrid squashe1
- Treatment
- 1 mgl−1 BAP + 0.1 mgl−1 NAAe4
- Outcome
- frequency of shooting and greater number of shoots per explante4
- Direction
- highere4
Not reportedComparator
Verbatim
e4“1 mgl−1 BAP + 0.1 mgl−1 NAA gave a good shoot growth with higher frequency of shooting and greater number of shoots per explant”
e1“Shoot tip explants derived from 15-day old aseptically grown seedlings of the hybrid squash”
Claim · model-written summary
1 mg/l BAP with 0.1 mg/l NAA gave good shoot growth.
Not reportedComparator
Verbatim
e4“1 mgl−1 BAP + 0.1 mgl−1 NAA gave a good shoot growth with higher frequency of shooting and greater number of shoots per explant”
e1“Shoot tip explants derived from 15-day old aseptically grown seedlings of the hybrid squash”
Claim · model-written summary
Regenerated shoots of the hybrid squash rooted well in half-strength MS medium with IBA and NAA.
Verbatim
e5“Regenerated shoots were rooted well in half strength MS medium with IBA and NAA (0.1 mgl−1 each)”
e1“Shoot tip explants derived from 15-day old aseptically grown seedlings of the hybrid squash”
Claim · model-written summary
Shoot tip explants of the hybrid squash were induced to produce multiple shoots.
Verbatim
e6“were induced to produce multiple shoots on Murashige and Skoog's medium”
e1“Shoot tip explants derived from 15-day old aseptically grown seedlings of the hybrid squash”
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