Paper · 2013 · Current Trends in Biotechnology and Pharmacy

Direct Organogenesis and Plant Regeneration from Cotyledons of a Multipurpose Tree, Acacia mangium Willd.

Md Shahinozzaman; M. O. Faruq; Mahbuba Ferdous; et al.

4
claims
7
verbatim quotes
8
citations
4
research areas
—
Not open access

Abstract

Using cotyledons of 2-week old in vitro germinated seedlings, a rapid and efficient regeneration protocol was established for A. mangium on Murashige and Skoog (MS) basal medium. Cotyledons showed better direct shoot organogenesis producing maximum 10.88 ± 0.35 shoots per explant when cultured on MS medium supplemented with 2.0 μM BA and 1.0 μM NAA. Full strength of MS medium supplemented with IBA (8.0 μM) was found best for rooting of in vitro shoots. The rooted plantlets were then transferred to small pots containing a sterilized mixture of sand, garden soil and compost (1:1:1) for acclimatization where plantlets grew well with 65% survival rate.

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Claims 4

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

A regeneration protocol was established from cotyledons of Acacia mangium.

Taxon
A. mangiumAcacia mangiume0
Outcome
regeneration protocole0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Acacia mangium
Acacia mangium · TaxonRodrigo Padula · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a rapid and efficient regeneration protocol was established for A. mangium on Murashige and Skoog (MS) basal medium.”

✓ located verbatim in the source · abstract, characters 62–178 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cotyledons on MS medium with BA and NAA produced a maximum of 10.88 ± 0.35 shoots per explant.

Taxon
Acacia mangiumAcacia mangiumt0
Organ / stage
Cotyledonse0
Treatment
MS medium supplemented with 2.0 μM BA and 1.0 μM NAAe0
Outcome
shoots per explante0
Direction
maximume0
Value
10.88 ± 0.35 shoots per explante0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Acacia mangium
Acacia mangium · TaxonRodrigo Padula · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Cotyledons showed better direct shoot organogenesis producing maximum 10.88 ± 0.35 shoots per explant when cultured on MS medium supplemented with 2.0 μM BA and 1.0 μM NAA.”

✓ located verbatim in the source · abstract, characters 179–351 · show in text ↑
t0

“Acacia mangium”

✓ located verbatim in the source · title, characters 84–98 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Full strength MS medium with 8.0 μM IBA was best for rooting in vitro shoots.

Taxon
Acacia mangiumAcacia mangiumt0
Treatment
Full strength of MS medium supplemented with IBA (8.0 μM)e0
Outcome
rooting of in vitro shootse0
Direction
beste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Acacia mangium
Acacia mangium · TaxonRodrigo Padula · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Full strength of MS medium supplemented with IBA (8.0 μM) was found best for rooting of in vitro shoots.”

✓ located verbatim in the source · abstract, characters 352–456 · show in text ↑
t0

“Acacia mangium”

✓ located verbatim in the source · title, characters 84–98 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Acclimatized plantlets had 65% survival in a sand, soil and compost mix.

Taxon
Acacia mangiumAcacia mangiumt0
Outcome
survival ratee0
Value
65%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Acacia mangium
Acacia mangium · TaxonRodrigo Padula · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“plantlets grew well with 65% survival rate.”

✓ located verbatim in the source · abstract, characters 613–656 · show in text ↑
t0

“Acacia mangium”

✓ located verbatim in the source · title, characters 84–98 · show in text ↑

indole-3-butyric acid · growth · Acacia mangiumResults agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

in vitro · growth · Acacia mangiumPositive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.