Abstract
Autoclaving eliminates microbial contamination during micropropagation, but the process is complex, time-consuming and expensive. Chemical sterilisation also effectively disinfects culture media and is relatively simple and cost-effective. Prior studies have focused on the effects of chemical sterilisation on bud induction, but the effects of sterilant on proliferation and rooting are unknown. We investigated the effect of sterilant on Acacia mangium × A. auriculiformis bud induction, rooting and subculture rooting. The optimal bud induction medium comprised 1/8 Murashige and Skoog medium + 7 g l−1 agar + 0.2 g l−1 chlorothalonil + 0.5 mg l−1 6-benzylaminopurine. The maximum bud induction rate (99.54%) with zero contamination was achieved using the third to fifth stem segments collected in October and treated with 0.8 g l−1 carbendazim for 3 min. The maximum rooting rate (97.62%) was attained using a rooting medium consisting of 7 g l−1 agar + 0.2 g l−1 chlorothalonil + 1.5 mg l−1 indolebutyric acid + 0.5 mg l−1 naphthaleneacetic acid. Proliferation ratio and subculture duration were positively correlated. The maximum proliferation rate (3.58%) was realised in the fourth subculture rooting. Chlorothalonil can effectively replace autoclaving of A. mangium × A. auriculiformis bud induction and rooting media. The present study provides insights for improving the rapid propagation method of Acacia sp. and a new direction for the development of micropropagation technology.
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Claims 7
Claim · model-written summary
The specified medium was optimal for bud induction.
- Taxon
- Acacia mangium × A. auriculiformis
Acacia mangiumt0 - Treatment
- 1/8 Murashige and Skoog medium + 7 g l−1 agar + 0.2 g l−1 chlorothalonil + 0.5 mg l−1 6-benzylaminopurinee0
- Outcome
- bud inductione0
- Direction
- optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimal bud induction medium comprised 1/8 Murashige and Skoog medium + 7 g l−1 agar + 0.2 g l−1 chlorothalonil + 0.5 mg l−1 6-benzylaminopurine”
t0“Acacia mangium × A. auriculiformis”
Claim · model-written summary
Carbendazim treatment of October stem segments gave 99.54% bud induction.
- Taxon
- Acacia mangium × A. auriculiformisAcacia mangiumt0
- Organ / stage
- third to fifth stem segmentse0
- Treatment
- 0.8 g l−1 carbendazim for 3 mine0
- Outcome
- bud induction ratee0
- Direction
- maximume0
- Value
- 99.54%e0
- Qualifier
- collected in Octobere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum bud induction rate (99.54%) with zero contamination was achieved using the third to fifth stem segments collected in October and treated with 0.8 g l−1 carbendazim for 3 min”
t0“Acacia mangium × A. auriculiformis”
Claim · model-written summary
The same treatment achieved zero contamination.
- Taxon
- Acacia mangium × A. auriculiformisAcacia mangiumt0
- Treatment
- 0.8 g l−1 carbendazim for 3 mine0
- Outcome
- contaminatione0
- Direction
- zeroe0
- Value
- zeroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum bud induction rate (99.54%) with zero contamination was achieved using the third to fifth stem segments collected in October and treated with 0.8 g l−1 carbendazim for 3 min”
t0“Acacia mangium × A. auriculiformis”
Claim · model-written summary
The specified rooting medium achieved 97.62% rooting.
- Taxon
- Acacia mangium × A. auriculiformisAcacia mangiumt0
- Treatment
- 7 g l−1 agar + 0.2 g l−1 chlorothalonil + 1.5 mg l−1 indolebutyric acid + 0.5 mg l−1 naphthaleneacetic acide0
- Outcome
- rooting ratee0
- Direction
- maximume0
- Value
- 97.62%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum rooting rate (97.62%) was attained using a rooting medium consisting of 7 g l−1 agar + 0.2 g l−1 chlorothalonil + 1.5 mg l−1 indolebutyric acid + 0.5 mg l−1 naphthaleneacetic acid”
t0“Acacia mangium × A. auriculiformis”
Claim · model-written summary
Proliferation ratio rose with subculture duration.
- Taxon
- Acacia mangium × A. auriculiformisAcacia mangiumt0
- Treatment
- subculture duratione0
- Outcome
- Proliferation ratioe0
- Direction
- positively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proliferation ratio and subculture duration were positively correlated”
t0“Acacia mangium × A. auriculiformis”
Claim · model-written summary
The fourth subculture rooting had maximum proliferation of 3.58%.
- Taxon
- Acacia mangium × A. auriculiformisAcacia mangiumt0
- Outcome
- proliferation ratee0
- Direction
- maximume0
- Value
- 3.58%e0
- Qualifier
- fourth subculture rootinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum proliferation rate (3.58%) was realised in the fourth subculture rooting”
t0“Acacia mangium × A. auriculiformis”
Claim · model-written summary
Chlorothalonil was judged an effective replacement for autoclaving bud induction and rooting media.
- Taxon
- A. mangium × A. auriculiformisAcacia mangiume0
- Treatment
- Chlorothalonile0
- Outcome
- bud induction and rooting mediae0
- Comparator
- autoclavinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chlorothalonil can effectively replace autoclaving of A. mangium × A. auriculiformis bud induction and rooting media”
The same questions in other papers
indole-3-butyric acid · growth · Acacia mangiumResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.