Abstract
Abstract Medium components and characteristics that affect the growth of embryogenic mango ( Mangifera indica L.) nucellar cultures and production of somatic embryos in vitro were studied. These included the role of complex organic supplements, basal medium formulations, solidifying agents, and liquid vs. solid media. Growth of embryogenic cultures in suspension was more efficient than on solid medium; however, subculture onto solid medium was essential for high-frequency production of morphologically normal somatic embryos, and Gelrite was more effective in this respect than Difco Bacto-agar. Modified B-5 basal medium was better for maintenance of cultures and for production of morphologically normal somatic embryos than either Murashige and Skoog or Woody Plant Medium. Sucrose concentrations at 5% to 6% were optimal for somatic embryo production, and also increased the frequency of recovery of normally differentiated early heart-shaped somatic embryos. Coconut water (20%, v/v) enhanced somatic embryo production by 18%; other complex organic addenda alone or in combination with coconut water were either ineffective (casein hydrolysate) or highly inhibitory (yeast extract) in comparison with basal medium alone.
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Claims 9
Claim · model-written summary
Suspension culture supported more efficient embryogenic mango culture growth than solid medium.
- Taxon
- Mango
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Mangifera indicat0 - Treatment
- suspensione0
- Outcome
- Growth of embryogenic culturese0
- Direction
- more efficiente0
- Comparator
- solid mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_from_India.jpg?width=500)
Verbatim
e0“Growth of embryogenic cultures in suspension was more efficient than on solid medium”
t0“Mango”
Claim · model-written summary
Subculture on solid medium was essential for high frequency production of normal somatic embryos.
- Taxon
- MangoMangifera indicat0
- Treatment
- subculture onto solid mediume0
- Outcome
- production of morphologically normal somatic embryose0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“subculture onto solid medium was essential for high-frequency production of morphologically normal somatic embryos”
t0“Mango”
Claim · model-written summary
Gelrite was more effective than Difco Bacto-agar for producing normal somatic embryos.
- Taxon
- MangoMangifera indicat0
- Treatment
- Gelritee0
- Outcome
- more effectivee0
- Direction
- more effectivee0
- Comparator
- Difco Bacto-agare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gelrite was more effective in this respect than Difco Bacto-agar”
t0“Mango”
Claim · model-written summary
Modified B-5 medium better maintained cultures and produced normal embryos than the two comparison media.
- Taxon
- MangoMangifera indicat0
- Treatment
- Modified B-5 basal mediume0
- Outcome
- maintenance of cultures and for production of morphologically normal somatic embryose0
- Direction
- bettere0
- Comparator
- Murashige and Skoog or Woody Plant Mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Modified B-5 basal medium was better for maintenance of cultures and for production of morphologically normal somatic embryos than either Murashige and Skoog or Woody Plant Medium”
t0“Mango”
Claim · model-written summary
Sucrose at 5% to 6% was optimal for somatic embryo production.
- Taxon
- MangoMangifera indicat0
- Treatment
- Sucrose concentrations at 5% to 6%e0
- Outcome
- somatic embryo productione0
- Direction
- optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose concentrations at 5% to 6% were optimal for somatic embryo production”
t0“Mango”
Claim · model-written summary
Sucrose at 5% to 6% increased recovery of normal early heart shaped embryos.
- Taxon
- MangoMangifera indicat0
- Treatment
- Sucrose concentrations at 5% to 6%e0
- Outcome
- frequency of recovery of normally differentiated early heart-shaped somatic embryose0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose concentrations at 5% to 6% were optimal for somatic embryo production, and also increased the frequency of recovery of normally differentiated early heart-shaped somatic embryos”
t0“Mango”
Claim · model-written summary
Coconut water enhanced somatic embryo production by 18%.
- Taxon
- MangoMangifera indicat0
- Treatment
- Coconut water (20%, v/v)e0
- Outcome
- somatic embryo productione0
- Direction
- enhancede0
- Value
- 18%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coconut water (20%, v/v) enhanced somatic embryo production by 18%”
t0“Mango”
Claim · model-written summary
Casein hydrolysate was ineffective relative to basal medium.
- Taxon
- MangoMangifera indicat0
- Treatment
- casein hydrolysatee0
- Outcome
- ineffectivee0
- Direction
- ineffectivee0
- Comparator
- basal medium alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“other complex organic addenda alone or in combination with coconut water were either ineffective (casein hydrolysate) or highly inhibitory (yeast extract) in comparison with basal medium alone”
t0“Mango”
Claim · model-written summary
Yeast extract was highly inhibitory relative to basal medium.
- Taxon
- MangoMangifera indicat0
- Treatment
- yeast extracte0
- Outcome
- highly inhibitorye0
- Direction
- highly inhibitorye0
- Comparator
- basal medium alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“other complex organic addenda alone or in combination with coconut water were either ineffective (casein hydrolysate) or highly inhibitory (yeast extract) in comparison with basal medium alone”
t0“Mango”
The same questions in other papers
sucrose · yield · Mangifera indica (mango)Results agree
2 papers in the same direction as this paper
- positive
- positive
sucrose · growth · Mangifera indica (mango)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →in vitro · yield · Mangifera indica (mango)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.