Abstract
During the last three decades, concentration of ozone in the lower troposphere of the Northern hemisphere has increased considerably. In many regions of the world there is an incontrovertible evidence that O3 levels are high enough to reduce crop yields, to cause shift in the genetic composition of natural and seminatural vegetation and to contribute to forest decline. These data explain the interest towards the damaging action of this pollutant on plants, particularly on molecular mechanisms, which are the basis of this kind of stress. An expression profile of genes up- and down-regulated by an acute ozone treatment (a single pulse of 150 ppb for five hours) in two hybrid poplar clones (Populus deltoides x maximowiczii, Eridano clone, and Populus x euoramericana, I-214 clone, sensitive and tolerant to O3, respectively) was obtained by sequencing 42 PCR clones belonging to four subtractive cDNA libraries obtained using suppression subtractive hybridisation (SSH). With the information gathered from different databases we were able to identify and assign a putative function to 31% of sequenced clones. The remain clones, classified as unknown, have presented an insufficient nucleotidic or deduced amino acidic similarity to proteins and/or genes of known function. Some of identified sequences presented high similarity with genes related to ozone stress already described in other species. Most of differentially expressed cDNA sequences can be classified as rarely transcribed. Indeed, almost half of the known sequences presented a meaningful similarity with genes not associated to ozone stress so far. Expression analysis of several sequenced up-regulated cDNA clones with known identity have showed that the level of all analyzed transcripts was increased by ozone treatment in both tolerant and sensitive poplar clones. By contrast, wall associated kinase (Ft32C clone) was induced by ozone stress in I-214 clone only. The Ft35A clone, isolated in I-214 poplar clone, was identified on the basis of high sequence similarity to Arabidopsis thaliana cytoplasmic ascorbate peroxidase (APX) cs1. This kind of APX result up-regulated by acute ozone treatment in tolerant poplar. By contrast, the cytosolic APX cs2, isolated by RT-PCR, was not induced by ozone treatment neither in tolerant nor in sensitive poplar clones.
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Claims 11
Claim · model-written summary
Putative function was assigned to 31% of the sequenced clones in the poplar ozone-response expression profile.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus spp.”
e0“With the information gathered from different databases we were able to identify and assign a putative function to 31% of sequenced clones”
Claim · model-written summary
Most differentially expressed cDNA sequences in poplar can be classified as rarely transcribed.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus spp.”
e0“Most of differentially expressed cDNA sequences can be classified as rarely transcribed”
Claim · model-written summary
Almost half of the known poplar sequences showed meaningful similarity with genes not previously associated with ozone stress.
- Outcome
- known sequencese0
- Value
- almost halfe0
- Qualifier
- meaningful similarity with genes not associated to ozone stress so fare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus spp.”
e0“almost half of the known sequences presented a meaningful similarity with genes not associated to ozone stress so far”
Claim · model-written summary
Some identified poplar sequences showed high similarity with genes related to ozone stress described in other species.
- Outcome
- high similarity with genes related to ozone stresse0
- Qualifier
- already described in other speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus spp.”
e0“Some of identified sequences presented high similarity with genes related to ozone stress already described in other species”
Claim · model-written summary
Ozone treatment increased the level of all analysed transcripts in the tolerant I-214 poplar clone.
- Material
- I-214 clonee1
- Treatment
- ozone treatmente0
- Outcome
- level of all analyzed transcriptse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus spp.”
e0“the level of all analyzed transcripts was increased by ozone treatment in both tolerant and sensitive poplar clones”
e1“Eridano clone, and Populus x euoramericana, I-214 clone, sensitive and tolerant to O3, respectively”
Claim · model-written summary
Ozone treatment increased the level of all analysed transcripts in the sensitive Eridano poplar clone.
- Material
- Eridano clonee1
- Treatment
- ozone treatmente0
- Outcome
- level of all analyzed transcriptse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus spp.”
e0“the level of all analyzed transcripts was increased by ozone treatment in both tolerant and sensitive poplar clones”
e1“Eridano clone, and Populus x euoramericana, I-214 clone, sensitive and tolerant to O3, respectively”
Claim · model-written summary
Ozone stress induced wall associated kinase (Ft32C clone) in the I-214 poplar clone only.
- Material
- I-214 clonee0
- Treatment
- ozone stresse0
- Gene
- wall associated kinasee0
- Outcome
- wall associated kinase (Ft32C clone)e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus spp.”
e0“wall associated kinase (Ft32C clone) was induced by ozone stress in I-214 clone only”
Claim · model-written summary
Clone Ft35A from the I-214 poplar clone shows high sequence similarity to Arabidopsis cytoplasmic ascorbate peroxidase (APX) cs1.
- Material
- I-214 poplar clonee0
- Gene
- cytoplasmic ascorbate peroxidase (APX) cs1e0
- Outcome
- high sequence similaritye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus spp.”
e0“The Ft35A clone, isolated in I-214 poplar clone, was identified on the basis of high sequence similarity to Arabidopsis thaliana cytoplasmic ascorbate peroxidase (APX) cs1”
Claim · model-written summary
Acute ozone treatment up-regulated APX in the tolerant poplar clone.
- Material
- tolerant poplare0
- Treatment
- acute ozone treatmente0
- Gene
- APXe0
- Outcome
- APXe0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus spp.”
e0“This kind of APX result up-regulated by acute ozone treatment in tolerant poplar”
Claim · model-written summary
Cytosolic APX cs2 was not induced by ozone treatment in the tolerant poplar clone.
- Material
- tolerante0
- Treatment
- ozone treatmente0
- Gene
- cytosolic APX cs2e0
- Outcome
- cytosolic APX cs2e0
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus spp.”
e0“the cytosolic APX cs2, isolated by RT-PCR, was not induced by ozone treatment neither in tolerant nor in sensitive poplar clones”
Claim · model-written summary
Cytosolic APX cs2 was not induced by ozone treatment in the sensitive poplar clone.
- Material
- sensitivee0
- Treatment
- ozone treatmente0
- Gene
- cytosolic APX cs2e0
- Outcome
- cytosolic APX cs2e0
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus spp.”
e0“the cytosolic APX cs2, isolated by RT-PCR, was not induced by ozone treatment neither in tolerant nor in sensitive poplar clones”
The same questions in other papers
ozone · physiology: antioxidant enzymes · Populus (poplar)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.