Abstract
This study aimed to determine the best combinations of plant growth regulators and other conditions in order to achieve organogenesis and multiplication directly from shoot tips of date palm (Phoenix dactylifera L.) var. Maktoom without callus formation so as to avoid any possibility of undesirable genetic variability. Results revealed that MS modified medium supplemented with 2.0 mg/L 2ip, 1.0 mg/L BA, 1.0 mg/L NAA and 1.0 mg/L NOA was the best for bud formation from shoot tip after 16 weeks (6.2 bud per explant). Subculturing the formed buds on liquid agitated multiplication medium supplemented with 4.0 mg/L 2ip, 2 mg/L BA, 1.0 mg/L NAA and 1.0 mg/L NOA gave the optimum average of buds number (12.6 buds). In elongation stage MS medium with 0.5 mg/L GA3 and 0.1 mg/L NAA enhanced plantlet length to 5.3 cm. Optimum rooting percentage 90% was achieved when shoots were transferred to a medium with 1.0 mg/L NAA. The average root number after 8 weeks was 5.4 with 9.0 cm length. Rooted shoots (plantlets) were transplanted in small pots containing a mixture of peatmoss and perlite (2:1) and placed in plastic tunnels or in a greenhouse. The survival percentage was 85% after 3 months when the plants were transferred to bigger pots. These results define a successful protocol for the in vitro propagation of Maktoom cv. date palm.
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Claims 7
Claim · model-written summary
Modified MS medium with 2ip, BA, NAA and NOA gave the best bud formation at 16 weeks, 6.2 buds per explant.
- Taxon
- date palm
Phoenix dactyliferat0 - Material
- Maktoomt0
- Organ / stage
- shoot tipe0
- Treatment
- MS modified medium supplemented with 2.0 mg/L 2ip, 1.0 mg/L BA, 1.0 mg/L NAA and 1.0 mg/L NOAe0
- Outcome
- bud formatione0
- Direction
- beste0
- Value
- 6.2 bud per explante0
- Qualifier
- after 16 weekse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS modified medium supplemented with 2.0 mg/L 2ip, 1.0 mg/L BA, 1.0 mg/L NAA and 1.0 mg/L NOA was the best for bud formation from shoot tip after 16 weeks (6.2 bud per explant)”
t0“date palm (Phoenix dactylifera L.) var. Maktoom”
Claim · model-written summary
Liquid agitated multiplication medium with 2ip, BA, NAA and NOA produced an optimum 12.6 buds.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Maktoomt0
- Treatment
- liquid agitated multiplication medium supplemented with 4.0 mg/L 2ip, 2 mg/L BA, 1.0 mg/L NAA and 1.0 mg/L NOAe0
- Outcome
- average of buds numbere0
- Direction
- optimume0
- Value
- 12.6 budse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subculturing the formed buds on liquid agitated multiplication medium supplemented with 4.0 mg/L 2ip, 2 mg/L BA, 1.0 mg/L NAA and 1.0 mg/L NOA gave the optimum average of buds number (12.6 buds)”
t0“date palm (Phoenix dactylifera L.) var. Maktoom”
Claim · model-written summary
MS medium with GA3 and NAA enhanced plantlet length to 5.3 cm.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Maktoomt0
- Treatment
- MS medium with 0.5 mg/L GA3 and 0.1 mg/L NAAe0
- Outcome
- plantlet lengthe0
- Direction
- enhancede0
- Value
- 5.3 cme0
- Qualifier
- In elongation stagee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In elongation stage MS medium with 0.5 mg/L GA3 and 0.1 mg/L NAA enhanced plantlet length to 5.3 cm”
t0“date palm (Phoenix dactylifera L.) var. Maktoom”
Claim · model-written summary
Medium with 1.0 mg/L NAA produced optimum rooting of 90%.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Maktoomt0
- Organ / stage
- shootse0
- Treatment
- a medium with 1.0 mg/L NAAe0
- Outcome
- rooting percentagee0
- Direction
- Optimume0
- Value
- 90%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimum rooting percentage 90% was achieved when shoots were transferred to a medium with 1.0 mg/L NAA”
t0“date palm (Phoenix dactylifera L.) var. Maktoom”
Claim · model-written summary
After eight weeks, mean root number was 5.4.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Maktoomt0
- Outcome
- average root numbere0
- Value
- 5.4e0
- Qualifier
- after 8 weekse0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The average root number after 8 weeks was 5.4 with 9.0 cm length”
t0“date palm (Phoenix dactylifera L.) var. Maktoom”
Claim · model-written summary
After eight weeks, root length was 9.0 cm.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Maktoomt0
- Outcome
- lengthe0
- Value
- 9.0 cme0
- Qualifier
- after 8 weekse0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The average root number after 8 weeks was 5.4 with 9.0 cm length”
t0“date palm (Phoenix dactylifera L.) var. Maktoom”
Claim · model-written summary
After transfer to larger pots, 85% of date palm plantlets survived for three months.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Maktoomt0
- Treatment
- transferred to bigger potse0
- Outcome
- survival percentagee0
- Value
- 85%e0
- Qualifier
- after 3 monthse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The survival percentage was 85% after 3 months when the plants were transferred to bigger pots”
t0“date palm (Phoenix dactylifera L.) var. Maktoom”
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