Abstract
Abstract The presence of the rare free amino acid octopine in different plant material was tested in order to confirm its validity as a specific marker for the expression of bacterial genetic information in tumors produced by infection with A grobacteriu m tu m efaciens. This guanidine derivative is present in auxin-autotrophic fast growing callus cultures of C repis capillaris, and N icotiana tabacum var. ''White Burley", all transformed by infection with A grobacteriu m tum efaciens. In auxin-hetero-trophic callus cultures and in healthy plant material of the same species octopine could not be found. In all A . tum efaciens strains including the avirulent No. 55 tested this guanidine derivative was present. However, since octopine was found in fast growing "habituated" cultures of N . taba cum var. "White Burley" and, in small amounts in root tips of pea and bean seedlings and not in a slow growing "transformed" strain T5 of C repis, it cannot be considered as a specific marker for the expression of genetic material from the transforming bacteria
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Claims 9
Claim · model-written summary
Octopine occurred in transformed Crepis callus.
- Taxon
- C repis capillarise0
- Interactor
- A grobacteriu m tu m efaciense0pathogen
- Outcome
- guanidine derivative is presente0
Not reportedDirection
Verbatim
e0“This guanidine derivative is present in auxin-autotrophic fast growing callus cultures of C repis capillaris, and N icotiana tabacum var. ''White Burley", all transformed by infection with A grobacteriu m tu m efaciens.”
Claim · model-written summary
Octopine occurred in transformed Nicotiana callus.
- Taxon
- N icotiana tabacume0
- Interactor
- A grobacteriu m tu m efaciense0pathogen
- Outcome
- guanidine derivative is presente0
Not reportedDirection
Verbatim
e0“This guanidine derivative is present in auxin-autotrophic fast growing callus cultures of C repis capillaris, and N icotiana tabacum var. ''White Burley", all transformed by infection with A grobacteriu m tu m efaciens.”
Claim · model-written summary
Octopine was absent in auxin heterotrophic and healthy material.
Not reportedDirection
Verbatim
e0“In auxin-hetero-trophic callus cultures and in healthy plant material of the same species octopine could not be found.”
e1“callus cultures of C repis capillaris”
Claim · model-written summary
Octopine was present in all tested Agrobacterium strains.
- Taxon
- A . tum efaciense0
- Outcome
- A . tum efaciens strains including the avirulent No. 55 tested this guanidine derivative was presente0
Not reportedDirection
Verbatim
e0“In all A . tum efaciens strains including the avirulent No. 55 tested this guanidine derivative was present.”
Claim · model-written summary
Octopine occurred in habituated tobacco cultures.
Not reportedDirection
Verbatim
e0“octopine was found in fast growing "habituated" cultures of N . taba cum var. "White Burley"”
Claim · model-written summary
Octopine occurred in pea root tips.
- Taxon
- pea
Lathyrus oleraceuse0 - Outcome
- in small amountse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in small amounts in root tips of pea and bean seedlings”
Claim · model-written summary
Octopine occurred in bean root tips.
Not reportedDirection
Verbatim
e0“in small amounts in root tips of pea and bean seedlings”
Claim · model-written summary
Octopine was absent from a slow growing transformed Crepis strain.
Not reportedDirection
Verbatim
e0“not in a slow growing "transformed" strain T5 of C repis”
Claim · model-written summary
Octopine was rejected as a specific bacterial transformation marker.
- Taxon
- C repis capillarise1
- Outcome
- specific marker for the expression of genetic materiale0
- Comparator
- transforming bacteriae0
Not reportedDirection
Verbatim
e0“it cannot be considered as a specific marker for the expression of genetic material from the transforming bacteria”
e1“callus cultures of C repis capillaris”