Abstract
SummaryTwo types of Balloon Type Bubble Bioreactors (BTBB, air lift type), continuous immersion cultures (with and without raft) and temporary immersion cultures (ebb and flow) were used for mass multiplication of shoots and bulblets of Garlic ‘Danyang’. Murashige and Skoog (1962) liquid medium supplemented with 2% sucrose was used for shoot proliferation and shoot proliferation was recorded after three weeks. MS liquid medium supplemented with 11% sucrose and 0.1 mg l–1 naphthalene acetic acid was used for bulblet induction and growth and bulblet growth was recorded after nine weeks in bioreactor cultures. The continuous immersion types of bioreactors were found suitable for shoot proliferation and 27 shoots were developed from each explant. Biomass of shoots was also highest by this method. Inoculation density of 15 shoots per culture was optimal and aeration of bioreactors with a sparger (0.1 air volume per medium per min) influenced the biomass production. Immersion type (with raft) bioreactor was optimal for bulblet formation. The ratio of inoculum size and media volume affected the growth values. Cold treatment of cultures at 4°C for three weeks enhanced the formation of bulblets. Bulblets were harvested at the end of the culture period, were given a cold treatment of 4°C for eight weeks and transferred to a potting mixture of peat moss and perlite (1:1). Bulblets more than 0.2 g sprouted (90%) within two weeks of transplantation.
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Claims 8
Claim · model-written summary
Continuous immersion bioreactors were suitable for shoot proliferation, with 27 shoots developed from each explant.
- Taxon
- Allium sativum
Allium sativumt0 - Treatment
- continuous immersion types of bioreactorse1
- Outcome
- shoot proliferatione1
- Value
- 27 shootse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“garlic (Allium sativumL.)”
e1“The continuous immersion types of bioreactors were found suitable for shoot proliferation and 27 shoots were developed from each explant”
Claim · model-written summary
Biomass of shoots was highest in continuous immersion bioreactors.
- Taxon
- Allium sativumAllium sativumt0
- Treatment
- continuous immersion types of bioreactorse1
- Outcome
- Biomass of shootse2
- Direction
- higheste2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“garlic (Allium sativumL.)”
e1“The continuous immersion types of bioreactors were found suitable for shoot proliferation and 27 shoots were developed from each explant”
e2“Biomass of shoots was also highest by this method”
Claim · model-written summary
An inoculation density of 15 shoots per culture was optimal.
- Taxon
- Allium sativumAllium sativumt0
- Outcome
- Inoculation densitye3
- Value
- 15 shoots per culturee3
- Qualifier
- optimale3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“garlic (Allium sativumL.)”
e3“Inoculation density of 15 shoots per culture was optimal”
Claim · model-written summary
Aeration of bioreactors with a sparger influenced biomass production.
- Taxon
- Allium sativumAllium sativumt0
- Treatment
- aeration of bioreactors with a sparger (0.1 air volume per medium per min)e4
- Outcome
- biomass productione4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“garlic (Allium sativumL.)”
e4“aeration of bioreactors with a sparger (0.1 air volume per medium per min) influenced the biomass production”
Claim · model-written summary
Immersion (with raft) bioreactor was optimal for bulblet formation.
- Taxon
- Allium sativumAllium sativumt0
- Treatment
- Immersion type (with raft) bioreactore5
- Outcome
- bulblet formatione5
- Direction
- optimale5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“garlic (Allium sativumL.)”
e5“Immersion type (with raft) bioreactor was optimal for bulblet formation”
Claim · model-written summary
The ratio of inoculum size to media volume affected growth values.
- Taxon
- Allium sativumAllium sativumt0
- Treatment
- ratio of inoculum size and media volumee6
- Outcome
- growth valuese6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“garlic (Allium sativumL.)”
e6“The ratio of inoculum size and media volume affected the growth values”
Claim · model-written summary
Cold treatment of cultures at 4°C for three weeks enhanced bulblet formation.
- Taxon
- Allium sativumAllium sativumt0
- Treatment
- Cold treatment of cultures at 4°C for three weekse7
- Outcome
- formation of bulbletse7
- Direction
- enhancede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“garlic (Allium sativumL.)”
e7“Cold treatment of cultures at 4°C for three weeks enhanced the formation of bulblets”
Claim · model-written summary
Bulblets over 0.2 g sprouted (90%) within two weeks of transplantation.
- Taxon
- Allium sativumAllium sativumt0
- Organ / stage
- Bulbletse0
- Outcome
- sproutede0
- Value
- 90%e0
- Qualifier
- more than 0.2 ge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“garlic (Allium sativumL.)”
e0“Bulblets more than 0.2 g sprouted (90%) within two weeks of transplantation”
The same questions in other papers
application · growth · Allium sativum (garlic)Positive and negative results
36 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
34 papers more in this group
See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.