Abstract
Present investigation was carried out to transform indica rice with Bt genes. Two indica genotypes viz., Swarna and APMS6B were used for tissue culture and transformation experiments. Callus induction frequency in MS medium in light was higher than in dark in both the genotypes. Callus was induced early in light incubated cultures. However no significant difference in plant regeneration was observed irrespective of callus source used. Calli derived from mature seeds of two genotypes were subjected to Agrobacterium mediated transformation using two gene constructs-EHA105/pCAMBIA Ac with cry 1 Ac and EHA105/pCAMBIA 1300 UBI-1B with cry 1B. The transient gus expression was more in Swarna. Successful transformation depended on factors including concentration of acetosyringone, concentration of Agrobacterium cells in culture, infiltration time and hygromycin in selection medium.
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Claims 5
Claim · model-written summary
Callus induction in light exceeded dark in both rice genotypes.
- Taxon
- RICE
Oryza sativat0 - Treatment
- lighte0
- Outcome
- Callus induction frequencye0
- Direction
- highere0
- Comparator
- darke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus induction frequency in MS medium in light was higher than in dark in both the genotypes”
t0“RICE”
Claim · model-written summary
Callus appeared earlier in light incubated cultures.
- Taxon
- RICEOryza sativat0
- Treatment
- lighte0
- Outcome
- Calluse0
- Direction
- earlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus was induced early in light incubated cultures”
t0“RICE”
Claim · model-written summary
Plant regeneration did not differ significantly by callus source.
- Taxon
- RICEOryza sativat0
- Treatment
- callus sourcee0
- Outcome
- plant regeneratione0
- Direction
- no significant differencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no significant difference in plant regeneration was observed irrespective of callus source used”
t0“RICE”
Claim · model-written summary
Transient gus expression was greater in Swarna.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transient gus expression was more in Swarna”
t0“RICE”
Claim · model-written summary
Transformation success depended on acetosyringone concentration, cell concentration, infiltration time and selection hygromycin.
- Taxon
- RICEOryza sativat0
- Treatment
- concentration of acetosyringonee0
- Outcome
- Successful transformatione0
- Qualifier
- concentration of Agrobacterium cells in culture, infiltration time and hygromycin in selection mediume0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Successful transformation depended on factors including concentration of acetosyringone, concentration of Agrobacterium cells in culture, infiltration time and hygromycin in selection medium”
t0“RICE”
The same questions in other papers
light · growth · Oryza sativa (rice)Positive and negative results
54 papers in the same direction as this paper · 38 in the opposite direction · 2 reporting no difference from an alternative
- no effect
- negative
- negative
91 papers more in this group
See all 95 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.