Abstract
Thevacuolar membrane (tonoplast) contains anabundant intrinsicprotein withsixmembrane-spanning domains that isencoded byasmall genefamily. Different isoforms oftonoplast intrinsic protein (TIP) areexpressed indifferent tissues orasaresult of specific signals. Usingpromoter-,B-glucuronidase (GUS)fusions andinsitu hybridization, wehaveexamined theexpression ofyTIPinArabidopsis thaliana. GUSstaining ofplants transformed withpromoter-GUS fusions showedthat'y-TIP geneexpression is highinrecently formed tissues ofyoungroots. Intheshoot, 'v-TIP geneexpression washighest inthevascular bundles ofstems and petioles, aswellasinthestipules andinthereceptacle ofthe flower. NoGUSactivity wasdetected inrootorshoot meristems orinolder tissues, suggesting temporal control of'v-TIP gene expression associated withcell elongation and/or differentiation. Insitu hybridization carried outwithwholeseedlings confirmed that inroottips, '-TIP mRNAwaspresent only inthezoneofcell elongation just behind theapical meristem. Inseedling shoots, mRNAabundance wasalso found tobecorrelated withcell expansion. These results indicate that'y-TIP maybeexpressed primarily atthetimewhenthelarge central vacuoles arebeing formed during cell enlargement.
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Claims 6
Claim · model-written summary
TIP gene expression was high in recently formed young root tissues.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Organ / stage
- youngrootse0
- Gene
- 'y-TIPe0
- Outcome
- geneexpressione0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GUSstaining ofplants transformed withpromoter-GUS fusions showedthat'y-TIP geneexpression is highinrecently formed tissues ofyoungroots.”
e1“yTIPinArabidopsis thaliana.”
Claim · model-written summary
Shoot TIP expression was highest in vascular bundles, stipules and the flower receptacle.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Organ / stage
- vascular bundles ofstems and petiolese0
- Gene
- 'v-TIPe0
- Outcome
- geneexpressione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Intheshoot, 'v-TIP geneexpression washighest inthevascular bundles ofstems and petioles, aswellasinthestipules andinthereceptacle ofthe flower.”
e1“yTIPinArabidopsis thaliana.”
Claim · model-written summary
No GUS activity was detected in root or shoot meristems or older tissues.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Organ / stage
- rootorshoot meristems orinolder tissuese0
- Outcome
- NoGUSactivity wasdetectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NoGUSactivity wasdetected inrootorshoot meristems orinolder tissues”
e1“yTIPinArabidopsis thaliana.”
Claim · model-written summary
In root tips, TIP mRNA occurred only in the elongation zone behind the apical meristem.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Organ / stage
- zoneofcell elongatione0
- Gene
- '-TIPe0
- Outcome
- mRNAwaspresent only inthezoneofcell elongatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inroottips, '-TIP mRNAwaspresent only inthezoneofcell elongation just behind theapical meristem.”
e1“yTIPinArabidopsis thaliana.”
Claim · model-written summary
Seedling shoot TIP mRNA abundance correlated with cell expansion.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Organ / stage
- seedling shootse0
- Outcome
- mRNAabundancee0
- Qualifier
- cell expansione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inseedling shoots, mRNAabundance wasalso found tobecorrelated withcell expansion.”
e1“yTIPinArabidopsis thaliana.”
Claim · model-written summary
The authors infer TIP expression occurs mainly when central vacuoles form during cell enlargement.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- 'y-TIPe0
- Outcome
- maybeexpressed primarily atthetimewhenthelarge central vacuoles arebeing formede0
- Qualifier
- during cell enlargemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results indicate that'y-TIP maybeexpressed primarily atthetimewhenthelarge central vacuoles arebeing formed during cell enlargement.”
e1“yTIPinArabidopsis thaliana.”