Paper · 2014 · Current Trends in Biotechnology and Pharmacy

Impact of Heavy Metals (Cr, Pb and Sn) on In Vitro Seed Germination and Seedling Growth of Green Gram (Vigna radiata (L.) R. Wilczek)

T. Neelesh Babu; Duddela Varaprasad; Y. Hima Bindu; et al.

7
claims
14
verbatim quotes
5
citations
1
research areas
—
Not open access

Abstract

Heavy metal toxicity has been known for more than a century and focus on the effects of heavy metals particularly on plant growth and development has only recently received more attention due to increasing pollution day by day. The effects of chromium (Cr), lead (Pb) and tin (Sn) on green gram [Vigna radiata (L.) Wilczek] were studied in the present investigation under in vitro growth conditions. The doses of 1, 10, 20, 50 and 100 ppm were used in the present experiments. The percentage of seed germination and early seedling growth were affected by Cr, Pb and Sn at higher concentrations. It has been observed that the effect of chromium is very significant and a drastic decrease in seed germination was noticed as well as early seedling growth with increasing concentrations especially at 50 and 100 ppm when compared to other heavy metals. Both lead and tin exhibited gradual/moderate reduction in seed germination and shoot length with increasing concentrations. In contrast, an abnormal root growth was noticed in tin treatments. The content of total chlorophyll decreased with increasing chromium concentrations. Similarly, abnormal total chlorophyll content was noticed in both lead and tin treatments. This investigation may be useful to study plant growth patterns particularly in areas polluted with heavy metals.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Chromium caused a drastic decrease in seed germination and early seedling growth of green gram, especially at 50 and 100 ppm, compared with other heavy metals.

Treatment
chromiume1
Outcome
seed germinatione1
Direction
drastic decreasee1
Qualifier
at 50 and 100 ppme1
Comparator
other heavy metalse1
Setting
in vitro growth conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The effects of chromium (Cr), lead (Pb) and tin (Sn) on green gram [Vigna radiata (L.) Wilczek] were studied in the present investigation under in vitro growth conditions”

✓ located verbatim in the source · abstract, characters 228–398 · show in text ↑
e1

“It has been observed that the effect of chromium is very significant and a drastic decrease in seed germination was noticed as well as early seedling growth with increasing concentrations especially at 50 and 100 ppm when compared to other heavy metals”

✓ located verbatim in the source · abstract, characters 595–847 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Lead caused a gradual or moderate reduction in seed germination and shoot length of green gram with increasing concentrations.

Treatment
leade2
Outcome
seed germination and shoot lengthe2
Direction
gradual/moderate reductione2
Setting
in vitro growth conditionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The effects of chromium (Cr), lead (Pb) and tin (Sn) on green gram [Vigna radiata (L.) Wilczek] were studied in the present investigation under in vitro growth conditions”

✓ located verbatim in the source · abstract, characters 228–398 · show in text ↑
e2

“Both lead and tin exhibited gradual/moderate reduction in seed germination and shoot length with increasing concentrations”

✓ located verbatim in the source · abstract, characters 849–971 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Tin caused a gradual or moderate reduction in seed germination and shoot length of green gram with increasing concentrations.

Treatment
tine2
Outcome
seed germination and shoot lengthe2
Direction
gradual/moderate reductione2
Setting
in vitro growth conditionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The effects of chromium (Cr), lead (Pb) and tin (Sn) on green gram [Vigna radiata (L.) Wilczek] were studied in the present investigation under in vitro growth conditions”

✓ located verbatim in the source · abstract, characters 228–398 · show in text ↑
e2

“Both lead and tin exhibited gradual/moderate reduction in seed germination and shoot length with increasing concentrations”

✓ located verbatim in the source · abstract, characters 849–971 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Tin caused abnormal root growth in green gram.

Treatment
tin treatmentse3
Outcome
root growthe3
Setting
in vitro growth conditionse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The effects of chromium (Cr), lead (Pb) and tin (Sn) on green gram [Vigna radiata (L.) Wilczek] were studied in the present investigation under in vitro growth conditions”

✓ located verbatim in the source · abstract, characters 228–398 · show in text ↑
e3

“In contrast, an abnormal root growth was noticed in tin treatments”

✓ located verbatim in the source · abstract, characters 973–1039 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Total chlorophyll content of green gram decreased with increasing chromium concentrations.

Treatment
chromiume4
Outcome
content of total chlorophylle4
Direction
decreasede4
Setting
in vitro growth conditionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The effects of chromium (Cr), lead (Pb) and tin (Sn) on green gram [Vigna radiata (L.) Wilczek] were studied in the present investigation under in vitro growth conditions”

✓ located verbatim in the source · abstract, characters 228–398 · show in text ↑
e4

“The content of total chlorophyll decreased with increasing chromium concentrations”

✓ located verbatim in the source · abstract, characters 1041–1123 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Lead caused abnormal total chlorophyll content in green gram.

Treatment
leade5
Outcome
total chlorophyll contente5
Setting
in vitro growth conditionse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The effects of chromium (Cr), lead (Pb) and tin (Sn) on green gram [Vigna radiata (L.) Wilczek] were studied in the present investigation under in vitro growth conditions”

✓ located verbatim in the source · abstract, characters 228–398 · show in text ↑
e5

“Similarly, abnormal total chlorophyll content was noticed in both lead and tin treatments”

✓ located verbatim in the source · abstract, characters 1125–1214 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Tin caused abnormal total chlorophyll content in green gram.

Treatment
tine5
Outcome
total chlorophyll contente5
Setting
in vitro growth conditionse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The effects of chromium (Cr), lead (Pb) and tin (Sn) on green gram [Vigna radiata (L.) Wilczek] were studied in the present investigation under in vitro growth conditions”

✓ located verbatim in the source · abstract, characters 228–398 · show in text ↑
e5

“Similarly, abnormal total chlorophyll content was noticed in both lead and tin treatments”

✓ located verbatim in the source · abstract, characters 1125–1214 · show in text ↑

lead atom · growth · Vigna radiata (mung bean)Results differ

10 papers: 1 positive, 9 negative or no effect

8 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

6 papers more in this group

See all 10 papers →

chromium atom · physiology: pigments · Vigna radiata (mung bean)Positive and negative results

7 papers: 1 positive, 6 negative or no effect

5 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

3 papers more in this group

See all 7 papers →

chromium atom · growth · Vigna radiata (mung bean)Positive and negative results

11 papers: 5 positive, 6 negative or no effect

5 papers in the same direction as this paper · 5 in the opposite direction

  1. positive
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

lead atom · physiology: pigments · Vigna radiata (mung bean)Results agree

3 papers: 0 positive, 3 negative or no effect

2 papers in the same direction as this paper

  1. negative
  2. negative
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.