Abstract
SummaryThe effects of 0, 2, 4 or 8 µM thidiazuron (TDZ) and explant type on adventitious shoots regenerated on excised leaf disks, sepals (calyx) and petiole halves of greenhouse-grown ‘Bounty’ strawberry (Fragaria ananassa Duch.) were studied. A moderate concentration (2 – 4 µM TDZ) supported bud and shoot regeneration without an intermediary callus phase on young expanding sepals, or on leaf disks with the adaxial side touching the culture medium, or on petiole halves placed horizontally with their cut-sides in contact with the medium, all maintained for 14 d in the dark. Sepals proved more effective than leaf discs and petiole segments for regenerating shoots. Another experiment compared the effects of 0, 0.5, 1, 2 and 4 µM TDZ or zeatin on shoot proliferation and rooting of sepal-derived adventitious shoots. Media containing TDZ generally promoted more callus formation and suppressed elongation and rooting of shoots. Shoots proliferated and roots developed best when explants were cultured in medium supplemented with 1 or 2 µM zeatin. An in vitro cloning protocol that enables shoot proliferation and rooting of strawberry explants in the same medium is expected to be applied for commercial micropropagation.
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Claims 8
Claim · model-written summary
Moderate TDZ supported direct bud and shoot regeneration on expanding sepals.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Organ / stage
- young expanding sepalse0
- Treatment
- 2 – 4 µM TDZe0
- Outcome
- bud and shoot regeneration without an intermediary callus phasee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“A moderate concentration (2 – 4 µM TDZ) supported bud and shoot regeneration without an intermediary callus phase on young expanding sepals”
t0“strawberry”
Claim · model-written summary
Moderate TDZ supported direct bud and shoot regeneration on leaf disks placed adaxial side down.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- leaf diskse0
- Treatment
- 2 – 4 µM TDZe0
- Outcome
- bud and shoot regeneration without an intermediary callus phasee0
- Qualifier
- adaxial side touching the culture mediume0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A moderate concentration (2 – 4 µM TDZ) supported bud and shoot regeneration without an intermediary callus phase on young expanding sepals, or on leaf disks with the adaxial side touching the culture medium”
t0“strawberry”
Claim · model-written summary
Moderate TDZ supported direct regeneration on horizontally placed petiole halves.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- petiole halvese0
- Treatment
- 2 – 4 µM TDZe0
- Outcome
- bud and shoot regeneration without an intermediary callus phasee0
- Qualifier
- placed horizontally with their cut-sides in contact with the mediume0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A moderate concentration (2 – 4 µM TDZ) supported bud and shoot regeneration without an intermediary callus phase on young expanding sepals, or on leaf disks with the adaxial side touching the culture medium, or on petiole halves placed horizontally with their cut-sides in contact with the medium”
t0“strawberry”
Claim · model-written summary
Sepals regenerated shoots more effectively than leaf discs and petiole segments.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- Sepalse0
- Outcome
- regenerating shootse0
- Direction
- more effectivee0
- Comparator
- leaf discs and petiole segmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sepals proved more effective than leaf discs and petiole segments for regenerating shoots.”
t0“strawberry”
Claim · model-written summary
TDZ media generally promoted more callus formation.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- TDZe0
- Outcome
- callus formatione0
- Direction
- moree0
- Qualifier
- generallye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Media containing TDZ generally promoted more callus formation and suppressed elongation and rooting of shoots.”
t0“strawberry”
Claim · model-written summary
TDZ media generally suppressed shoot elongation and rooting.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- TDZe0
- Outcome
- elongation and rooting of shootse0
- Direction
- suppressede0
- Qualifier
- generallye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Media containing TDZ generally promoted more callus formation and suppressed elongation and rooting of shoots.”
t0“strawberry”
Claim · model-written summary
Shoots proliferated and roots developed best on 1 or 2 µM zeatin medium.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- 1 or 2 µM zeatine0
- Outcome
- Shoots proliferated and roots developede0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoots proliferated and roots developed best when explants were cultured in medium supplemented with 1 or 2 µM zeatin.”
t0“strawberry”
Claim · model-written summary
The authors expect the combined proliferation and rooting protocol to serve commercial micropropagation.
- Taxon
- strawberryFragaria ×ananassat0
- Outcome
- commercial micropropagatione0
- Qualifier
- expected to be appliede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An in vitro cloning protocol that enables shoot proliferation and rooting of strawberry explants in the same medium is expected to be applied for commercial micropropagation.”
t0“strawberry”
The same questions in other papers
Thidiazuron · growth · Fragaria x ananassa (strawberry)Positive and negative results
20 in the opposite direction
- positive
- positive
- positive
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.