Abstract
Abstract β-Cryptogein, a proteinaceous elicitor from the phytopathogenic fungus Phytophthoracryptogea , is known to induce leaf necrosis in tobacco and non-specific resistance (expressed in the perinecrotic leaf area) against a wide range of tobacco pathogens. To reveal mechanisms underlying the acquired resistance, biochemical changes in leaves of β-cryptogein-elicited tobacco were followed three, five and ten days after elicitation. The activities of peroxidase, β-1,3-glucanase and β-glucosidase, as well as the patterns of acidic pathogenesis-related (PR)-proteins were determined. The protected part (perinecrotic area) and the non-protected part (distant extra-perinecrotic area) of leaves of β-cryptogein-stem treated tobacco (cv. Xanthin.c.) were analyzed. Leaves of water-stem treated tobacco served as controls. It was shown that in the protected leaf part β-cryptogein caused significant metabolic shifts early after elicitation, persisting during the whole period studied. An important increase of peroxidase and β-1,3-glucanase activity was recorded. PR-protein components appeared that were absent in the controls. There were negligible changes in β-glucosidase activity. In the non-protected leaf part late and non-significant changes occurred. Taking into account the antimicrobial, regulatory and structure-modifying properties of the biochemical components studied, it may be admitted that β-cryptogein elicited the development of a hostile environment, i.e. a potential for plant resistance against subsequent pathogen invasion.
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Claims 7
Claim · model-written summary
β-cryptogein caused persistent metabolic shifts in protected leaf tissue.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Organ / stage
- protected leaf parte0
- Treatment
- β-cryptogeine0
- Outcome
- metabolic shiftse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“in the protected leaf part β-cryptogein caused significant metabolic shifts early after elicitation, persisting during the whole period studied”
Claim · model-written summary
Peroxidase activity increased in elicited protected leaves.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- peroxidasee0
- Outcome
- peroxidasee0
- Direction
- increasee0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“An important increase of peroxidase and β-1,3-glucanase activity was recorded”
Claim · model-written summary
β-1,3-glucanase activity increased in elicited protected leaves.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- β-1,3-glucanasee0
- Outcome
- β-1,3-glucanase activitye0
- Direction
- increasee0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“An important increase of peroxidase and β-1,3-glucanase activity was recorded”
Claim · model-written summary
PR-protein components appeared in protected tissue but were absent in controls.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- PR-proteine0
- Outcome
- PR-protein components appearede0
- Comparator
- controlse0
Not reportedTreatmentDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“PR-protein components appeared that were absent in the controls”
Claim · model-written summary
β-glucosidase activity changed negligibly.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- β-glucosidase activitye0
- Direction
- negligible changese0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“There were negligible changes in β-glucosidase activity”
Claim · model-written summary
The non-protected leaf part had late, nonsignificant changes.
- Taxon
- TobaccoNicotiana tabacumt0
- Organ / stage
- non-protected leaf parte0
- Outcome
- late and non-significant changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“In the non-protected leaf part late and non-significant changes occurred”
Claim · model-written summary
The authors suggest β-cryptogein elicited a hostile environment potentially resistant to later pathogens.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- β-cryptogeine0
- Outcome
- potential for plant resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“β-cryptogein elicited the development of a hostile environment, i.e. a potential for plant resistance against subsequent pathogen invasion”