Abstract
We evaluated the effect of several modifications of the detergent fiber method on nutritional analyses of 5 species each of herbaceous and woody plants. Samples of each species were subjected to the acid detergent fiber (ADF) analysis following the standard procedure and the sequential analysis procedure. Sequential ADF values were always lower than standard ADF values in both herbaceous and woody plants (P<0.01). Sequential ADF with sulfite was also lower than sequential ADF without sulfite (P<0.05). In woody plants, this was caused by a loss of lignin in the presence of sulfite. In the absence of sulfite, sequential ADF was not more efficient than standard ADF in removing proteins bound to cell walls, nor was it more efficient at estimating hemicellulose as the difference between neutral detergent fiber (NDF) and ADF
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Claims 5
Claim · model-written summary
Sequential ADF values were lower than standard ADF values in herbaceous and woody plants.
Verbatim
e0“Sequential ADF values were always lower than standard ADF values in both herbaceous and woody plants (P<0.01)”
Claim · model-written summary
Sequential ADF with sulfite was lower than sequential ADF without sulfite.
- Outcome
- Sequential ADF with sulfitee0
- Direction
- lowere0
- Value
- P<0.05e0
- Comparator
- sequential ADF without sulfitee0
Not reportedTaxon
Verbatim
e0“Sequential ADF with sulfite was also lower than sequential ADF without sulfite (P<0.05)”
t0“Fiber Method”
Claim · model-written summary
In woody plants, sulfite caused lignin loss.
Not reportedComparator
Verbatim
e0“In woody plants, this was caused by a loss of lignin in the presence of sulfite”
Claim · model-written summary
Without sulfite, sequential ADF was no more efficient than standard ADF at removing cell wall bound proteins.
- Outcome
- removing proteins bound to cell wallse0
- Direction
- not more efficiente0
- Qualifier
- absence of sulfitee0
- Comparator
- standard ADFe0
Not reportedTaxon
Verbatim
e0“In the absence of sulfite, sequential ADF was not more efficient than standard ADF in removing proteins bound to cell walls”
Claim · model-written summary
Without sulfite, sequential ADF was no more efficient than standard ADF for estimating hemicellulose.
- Outcome
- estimating hemicellulosee0
- Direction
- not more efficiente0
- Qualifier
- absence of sulfitee0
- Comparator
- standard ADFe0
Not reportedTaxon
Verbatim
e0“In the absence of sulfite, sequential ADF was not more efficient than standard ADF in removing proteins bound to cell walls, nor was it more efficient at estimating hemicellulose as the difference between neutral detergent fiber (NDF) and ADF”