Abstract
Indonesia is one of the countries which have abundant germplasm of pummelo (Citrus maxima (Burm.) Merr.). The pummelo germplasm must be conserved to prevent its extinction due to biotic and abiotic stresses. In vitro conservation using slow growth technique can be considered as an alternative of ex-situ conservation. Two experiments were conducted to obtain suitable medium to conserve pummelo in vitro. The first experiment was conservation using modified concentration of MS and sucrose. MS medium concentrations were 1/2MS and MS, while sucrose concentrations were 0, 1, 2 and 3%. The second experiment was conservation using osmoticum and retardant in MS medium. There were six combinations of MS medium supplemented with osmoticum and retardant, i.e. MS + sucrose 3%, MS + sucrose 3% + paclobutrazol 7.5 ppm, MS + sucrose 3% + paclobutrazol 15 ppm, MS + sorbitol 2%, MS + sorbitol 2% + paclobutrazol 7.5 ppm, MS + sorbitol 2% + paclobutrazol 15 ppm. The results showed that reducing MS medium and sucrose consentration decreased leaf number and shoot length but increased root number and length. The combination of osmoticum and retardant reduced shoot length, leaf number, root number and length. Based on the planlet visual and inhibition of growth through the decrease of leaf number, shoot and root length, the best medium to preserve pummelo was MS + sorbitol 2% + paclobutrazol 7.5 ppm.
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Claims 5
Claim · model-written summary
Reducing MS medium and sucrose concentration decreased pummelo leaf number and shoot length.
- Taxon
- pummelo
Citrus maximae1 - Treatment
- reducing MS medium and sucrose consentratione0
- Outcome
- leaf number and shoot lengthe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that reducing MS medium and sucrose consentration decreased leaf number and shoot length but increased root number and length.”
e1“pummelo (Citrus maxima (Burm.) Merr.)”
Claim · model-written summary
Reducing MS medium and sucrose concentration increased pummelo root number and length.
- Taxon
- pummeloCitrus maximae1
- Treatment
- reducing MS medium and sucrose consentratione0
- Outcome
- root number and lengthe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that reducing MS medium and sucrose consentration decreased leaf number and shoot length but increased root number and length.”
e1“pummelo (Citrus maxima (Burm.) Merr.)”
Claim · model-written summary
Combined osmoticum and retardant reduced pummelo shoot length and leaf number.
- Taxon
- pummeloCitrus maximae1
- Treatment
- combination of osmoticum and retardante0
- Outcome
- shoot length, leaf numbere0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The combination of osmoticum and retardant reduced shoot length, leaf number, root number and length.”
e1“pummelo (Citrus maxima (Burm.) Merr.)”
Claim · model-written summary
Combined osmoticum and retardant reduced pummelo root number and length.
- Taxon
- pummeloCitrus maximae1
- Treatment
- combination of osmoticum and retardante0
- Outcome
- root number and lengthe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The combination of osmoticum and retardant reduced shoot length, leaf number, root number and length.”
e1“pummelo (Citrus maxima (Burm.) Merr.)”
Claim · model-written summary
The authors recommend MS with 2% sorbitol and 7.5 ppm paclobutrazol as the best pummelo conservation medium based on appearance and growth inhibition.
- Taxon
- pummeloCitrus maximae0
- Outcome
- MS + sorbitol 2% + paclobutrazol 7.5 ppme0
- Qualifier
- planlet visual and inhibition of growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Based on the planlet visual and inhibition of growth through the decrease of leaf number, shoot and root length, the best medium to preserve pummelo was MS + sorbitol 2% + paclobutrazol 7.5 ppm.”