Abstract
Changes in activity of several hydrolytic enzymes were measured in extracts prepared from the cotyledons following germination of bean seeds (Phaseolus vulgaris cv. Hawkesbury Wonder). Aminopeptidase AP2 (phenanthroline-sensitive leucyl β-naphthylamidase), N-carbobenzoxy-L- tyrosine-p-nitrophenol ester (N-CBZ-L-Tyr-pNP) hydrolase, N-benzoyl-L-tyrosineethyl ester (BTEE) hydrolase and acid phosphatase all increased in activity per cotyledon, reaching maximum values after 4, 6, 10 and 10 days respectively. By comparison, aminopeptidase AP1 (phenanthrolineinsensitive leucyl β-naphthylamidase) showed maximum activity after 2 days and declined immediately. α-N-benzoyl-D,L-arginine-p-nitroanilide hydrolase activity was maintained at a maximum value between days 2 and 10 before declining. Differences in maximum activity per cotyledon, initial presence or absence, and timing of both increase and decrease of N-CBZ-L-Tyr-pNP and BTEE hydrolase activities suggest that at least two enzymes contribute to these activities, and that neither activity indicates the presence of a chymotrypsin-like endopeptidase.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Aminopeptidase AP2 activity per cotyledon increased after germination, reaching its maximum after 4 days.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarise0 - Organ / stage
- cotyledonse0
- Treatment
- following germinatione0
- Gene
- Aminopeptidase AP2e1
- Outcome
- activity per cotyledone1
- Direction
- increasede1
- Qualifier
- reaching maximum values after 4e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extracts prepared from the cotyledons following germination of bean seeds (Phaseolus vulgaris cv. Hawkesbury Wonder)”
e1“Aminopeptidase AP2 (phenanthroline-sensitive leucyl β-naphthylamidase), N-carbobenzoxy-L- tyrosine-p-nitrophenol ester (N-CBZ-L-Tyr-pNP) hydrolase, N-benzoyl-L-tyrosineethyl ester (BTEE) hydrolase and acid phosphatase all increased in activity per cotyledon, reaching maximum values after 4, 6, 10 and 10 days respectively”
Claim · model-written summary
N-CBZ-L-Tyr-pNP hydrolase activity per cotyledon increased after germination, reaching its maximum after 6 days.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Organ / stage
- cotyledonse0
- Treatment
- following germinatione0
- Gene
- N-CBZ-L-Tyr-pNPe1
- Outcome
- activity per cotyledone1
- Direction
- increasede1
- Qualifier
- reaching maximum values after 4, 6, 10 and 10 days respectivelye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extracts prepared from the cotyledons following germination of bean seeds (Phaseolus vulgaris cv. Hawkesbury Wonder)”
e1“Aminopeptidase AP2 (phenanthroline-sensitive leucyl β-naphthylamidase), N-carbobenzoxy-L- tyrosine-p-nitrophenol ester (N-CBZ-L-Tyr-pNP) hydrolase, N-benzoyl-L-tyrosineethyl ester (BTEE) hydrolase and acid phosphatase all increased in activity per cotyledon, reaching maximum values after 4, 6, 10 and 10 days respectively”
Claim · model-written summary
BTEE hydrolase activity per cotyledon increased after germination, reaching its maximum after 10 days.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Organ / stage
- cotyledonse0
- Treatment
- following germinatione0
- Gene
- BTEEe1
- Outcome
- activity per cotyledone1
- Direction
- increasede1
- Qualifier
- reaching maximum values after 4, 6, 10 and 10 days respectivelye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extracts prepared from the cotyledons following germination of bean seeds (Phaseolus vulgaris cv. Hawkesbury Wonder)”
e1“Aminopeptidase AP2 (phenanthroline-sensitive leucyl β-naphthylamidase), N-carbobenzoxy-L- tyrosine-p-nitrophenol ester (N-CBZ-L-Tyr-pNP) hydrolase, N-benzoyl-L-tyrosineethyl ester (BTEE) hydrolase and acid phosphatase all increased in activity per cotyledon, reaching maximum values after 4, 6, 10 and 10 days respectively”
Claim · model-written summary
Acid phosphatase activity per cotyledon increased after germination, reaching its maximum after 10 days.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Organ / stage
- cotyledonse0
- Treatment
- following germinatione0
- Gene
- acid phosphatasee1
- Outcome
- activity per cotyledone1
- Direction
- increasede1
- Qualifier
- reaching maximum values after 4, 6, 10 and 10 days respectivelye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extracts prepared from the cotyledons following germination of bean seeds (Phaseolus vulgaris cv. Hawkesbury Wonder)”
e1“Aminopeptidase AP2 (phenanthroline-sensitive leucyl β-naphthylamidase), N-carbobenzoxy-L- tyrosine-p-nitrophenol ester (N-CBZ-L-Tyr-pNP) hydrolase, N-benzoyl-L-tyrosineethyl ester (BTEE) hydrolase and acid phosphatase all increased in activity per cotyledon, reaching maximum values after 4, 6, 10 and 10 days respectively”
Claim · model-written summary
Aminopeptidase AP1 activity reached its maximum after 2 days and then declined immediately after germination.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Organ / stage
- cotyledonse0
- Gene
- aminopeptidase AP1e2
- Outcome
- activitye2
- Direction
- declinede2
- Qualifier
- showed maximum activity after 2 dayse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extracts prepared from the cotyledons following germination of bean seeds (Phaseolus vulgaris cv. Hawkesbury Wonder)”
e2“By comparison, aminopeptidase AP1 (phenanthrolineinsensitive leucyl β-naphthylamidase) showed maximum activity after 2 days and declined immediately”
Claim · model-written summary
α-N-benzoyl-D,L-arginine-p-nitroanilide hydrolase activity was maintained at a maximum between days 2 and 10 and then declined.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Organ / stage
- cotyledonse0
- Gene
- α-N-benzoyl-D,L-arginine-p-nitroanilide hydrolasee3
- Outcome
- activitye3
- Direction
- maintainede3
- Qualifier
- maximum value between days 2 and 10e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extracts prepared from the cotyledons following germination of bean seeds (Phaseolus vulgaris cv. Hawkesbury Wonder)”
e3“α-N-benzoyl-D,L-arginine-p-nitroanilide hydrolase activity was maintained at a maximum value between days 2 and 10 before declining”
Claim · model-written summary
Differences in the timing and magnitude of N-CBZ-L-Tyr-pNP and BTEE hydrolase activities suggest that at least two enzymes contribute to these activities.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Outcome
- hydrolase activitiese4
- Qualifier
- at least two enzymes contribute to these activitiese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extracts prepared from the cotyledons following germination of bean seeds (Phaseolus vulgaris cv. Hawkesbury Wonder)”
e4“Differences in maximum activity per cotyledon, initial presence or absence, and timing of both increase and decrease of N-CBZ-L-Tyr-pNP and BTEE hydrolase activities suggest that at least two enzymes contribute to these activities”