Abstract
Transgenic peanut (Arachis hypogaea L.) containing a gene designed for RNA interference (RNAi) showed stable complete silencing of Ara h 2 and partial silencing of Ara h 6, two potent peanut allergens/proteins, along with minimal collateral changes to other allergens, Ara h 1 and Ara h 3, across three generations (T3, T4, and T5) under field conditions. Different soil sulfur levels (0.012, 0.3, and 3.0 mM) differentially impacted sulfur-rich (Ara h 2, Ara h 3, and Ara h 6) versus sulfur-poor (Ara h 1) proteins in non-transgenic versus transgenic peanut. The sulfur level had no effect on Ara h 1, whereas low sulfur led to a significant reduction of Ara h 3 in transgenic and non-transgenic seeds and Ara h 2 and Ara h 6 in non-transgenic but not in transgenic peanuts because these proteins already were reduced by gene silencing. These results demonstrate stability of transgene expression and the potential utility of RNAi in allergen manipulation.
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Claims 7
Claim · model-written summary
Transgenic peanut with RNAi showed stable complete silencing of Ara h 2 under field conditions across three generations.
- Taxon
- peanut
Arachis hypogaeat0 - Treatment
- a gene designed for RNA interference (RNAi)e1
- Gene
- Ara h 2e1
- Outcome
- silencing of Ara h 2e1
- Direction
- stable complete silencinge1
- Qualifier
- across three generations (T3, T4, and T5)e1
- Setting
- field conditionse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reduced Allergen Peanut”
e1“Transgenic peanut (Arachis hypogaea L.) containing a gene designed for RNA interference (RNAi) showed stable complete silencing of Ara h 2 and partial silencing of Ara h 6, two potent peanut allergens/proteins, along with minimal collateral changes to other allergens, Ara h 1 and Ara h 3, across three generations (T3, T4, and T5) under field conditions.”
Claim · model-written summary
Transgenic peanut with RNAi showed partial silencing of Ara h 6.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- a gene designed for RNA interference (RNAi)e1
- Gene
- Ara h 6e1
- Outcome
- silencing of Ara h 6e1
- Direction
- partial silencinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reduced Allergen Peanut”
e1“Transgenic peanut (Arachis hypogaea L.) containing a gene designed for RNA interference (RNAi) showed stable complete silencing of Ara h 2 and partial silencing of Ara h 6, two potent peanut allergens/proteins, along with minimal collateral changes to other allergens, Ara h 1 and Ara h 3, across three generations (T3, T4, and T5) under field conditions.”
Claim · model-written summary
Transgenic peanut showed minimal collateral changes to the other allergens Ara h 1 and Ara h 3.
- Taxon
- peanutArachis hypogaeat0
- Gene
- Ara h 1e1
- Outcome
- collateral changes to other allergense1
- Qualifier
- minimale1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reduced Allergen Peanut”
e1“Transgenic peanut (Arachis hypogaea L.) containing a gene designed for RNA interference (RNAi) showed stable complete silencing of Ara h 2 and partial silencing of Ara h 6, two potent peanut allergens/proteins, along with minimal collateral changes to other allergens, Ara h 1 and Ara h 3, across three generations (T3, T4, and T5) under field conditions.”
Claim · model-written summary
Different soil sulfur levels differentially impacted sulfur-rich versus sulfur-poor Ara h proteins in non-transgenic versus transgenic peanut.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- Different soil sulfur levels (0.012, 0.3, and 3.0 mM)e2
- Outcome
- sulfur-rich (Ara h 2, Ara h 3, and Ara h 6) versus sulfur-poor (Ara h 1) proteinse2
- Direction
- differentially impactede2
- Comparator
- non-transgenic versus transgenic peanute2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reduced Allergen Peanut”
e2“Different soil sulfur levels (0.012, 0.3, and 3.0 mM) differentially impacted sulfur-rich (Ara h 2, Ara h 3, and Ara h 6) versus sulfur-poor (Ara h 1) proteins in non-transgenic versus transgenic peanut.”
Claim · model-written summary
The sulfur level had no effect on Ara h 1 protein.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- sulfur levele3
- Gene
- Ara h 1e3
- Outcome
- Ara h 1e3
- Direction
- no effecte3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reduced Allergen Peanut”
e3“The sulfur level had no effect on Ara h 1”
Claim · model-written summary
Low sulfur significantly reduced Ara h 3 in transgenic and non-transgenic seeds.
- Taxon
- peanutArachis hypogaeat0
- Organ / stage
- seedse4
- Treatment
- low sulfure4
- Outcome
- Ara h 3e4
- Direction
- significant reductione4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reduced Allergen Peanut”
e4“low sulfur led to a significant reduction of Ara h 3 in transgenic and non-transgenic seeds and Ara h 2 and Ara h 6 in non-transgenic but not in transgenic peanuts”
Claim · model-written summary
Low sulfur significantly reduced Ara h 2 and Ara h 6 in non-transgenic but not in transgenic peanut, because these proteins were already reduced by gene silencing in transgenic plants.
- Taxon
- peanutArachis hypogaeat0
- Material
- non-transgenice4
- Treatment
- low sulfure4
- Outcome
- Ara h 2 and Ara h 6e4
- Direction
- significant reductione4
- Qualifier
- because these proteins already were reduced by gene silencinge5
- Comparator
- transgenic peanutse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reduced Allergen Peanut”
e4“low sulfur led to a significant reduction of Ara h 3 in transgenic and non-transgenic seeds and Ara h 2 and Ara h 6 in non-transgenic but not in transgenic peanuts”
e5“because these proteins already were reduced by gene silencing”
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sulfur atom · symbiosis · Arachis hypogaea (peanut)Positive and negative results
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.