Abstract
As rice seeds are sometimes contaminated with phytopathogenic organisms such as blast disease fungi and nematodes, a novel non-chemical disinfection method for rice seeds is highly required. In order to develop a disinfection method, the effect of low energy electron (“soft-electrons”) on seed DNA was examined by using the neutral comet assay. Rice seeds (whole grain) were treated with electrons of different acceleration voltages (180kV to 1MV) at a dose of 5kGy. Nucleus suspensions were prepared from whole brown rice and subjected to electrophoresis. DNA from un-irradiated (control) seeds relaxed and produced comets with a short tail, most of the comets distributed within the range of comet length between 30μm to 70μm. In the case of seeds treated with electrons at acceleration voltages up to 190kV, cells without seed coats were not damaged and the frequency histograms of comet length showed almost the same pattern as that for control. At acceleration voltages higher than 200kV, the cells were distributed into two categories; DNA comets with a short tail (with little DNA damages, less than 70μm in the comet length, ) and DNA comets with long tails (with sever strand breaks, more than 130μm in the comet length). The ratios of damaged cells increased with increasing acceleration voltage. The growths of rice seedlings were not affected by the treatment with electrons at up to 200kV. On the contrary, the cells of gamma-irradiated seed showed small variations in the comet length, and which were depending on radiation dose. The individual cells of gamma-irradiated seeds at 1kGy showed shorter comet than the damaged cells with soft electron, seed treated with gamma rays (1-5kGy) did not shoot nor root.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Control rice seed DNA produced short comet tails, mostly 30μm to 70μm long.
- Taxon
- Rice
Oryza sativae1 - Treatment
- un-irradiated (control)e0
- Outcome
- comet lengthe0
- Value
- 30μm to 70μme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA from un-irradiated (control) seeds relaxed and produced comets with a short tail, most of the comets distributed within the range of comet length between 30μm to 70μm.”
e1“Rice seeds”
Claim · model-written summary
Electron treatments up to 190kV did not damage cells without seed coats.
- Taxon
- RiceOryza sativae1
- Treatment
- electrons at acceleration voltages up to 190kVe0
- Outcome
- cells without seed coats were not damagede0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the case of seeds treated with electrons at acceleration voltages up to 190kV, cells without seed coats were not damaged and the frequency histograms of comet length showed almost the same pattern as that for control.”
e1“Rice seeds”
Claim · model-written summary
Comet-length distributions for seeds treated up to 190kV resembled controls.
- Taxon
- RiceOryza sativae1
- Treatment
- electrons at acceleration voltages up to 190kVe0
- Outcome
- frequency histograms of comet lengthe0
- Direction
- almost the same patterne0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the case of seeds treated with electrons at acceleration voltages up to 190kV, cells without seed coats were not damaged and the frequency histograms of comet length showed almost the same pattern as that for control.”
e1“Rice seeds”
Claim · model-written summary
Above 200kV, electron treated cells included short-tail comets with little DNA damage.
- Taxon
- RiceOryza sativae1
- Treatment
- acceleration voltages higher than 200kVe0
- Outcome
- DNA comets with a short taile0
- Value
- less than 70μme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At acceleration voltages higher than 200kV, the cells were distributed into two categories; DNA comets with a short tail (with little DNA damages, less than 70μm in the comet length, ) and DNA comets with long tails (with sever strand breaks, more than 130μm in the comet length).”
e1“Rice seeds”
Claim · model-written summary
Above 200kV, electron treated cells included long-tail comets with severe strand breaks.
- Taxon
- RiceOryza sativae1
- Treatment
- acceleration voltages higher than 200kVe0
- Outcome
- DNA comets with long tailse0
- Value
- more than 130μme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At acceleration voltages higher than 200kV, the cells were distributed into two categories; DNA comets with a short tail (with little DNA damages, less than 70μm in the comet length, ) and DNA comets with long tails (with sever strand breaks, more than 130μm in the comet length).”
e1“Rice seeds”
Claim · model-written summary
The proportion of damaged cells rose as electron acceleration voltage increased.
- Taxon
- RiceOryza sativae1
- Treatment
- acceleration voltagee0
- Outcome
- ratios of damaged cellse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ratios of damaged cells increased with increasing acceleration voltage.”
e1“Rice seeds”
Claim · model-written summary
Electron treatment up to 200kV did not affect rice seedling growth.
- Taxon
- RiceOryza sativae1
- Treatment
- electrons at up to 200kVe0
- Outcome
- growths of rice seedlingse0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growths of rice seedlings were not affected by the treatment with electrons at up to 200kV.”
e1“Rice seeds”
Claim · model-written summary
Gamma irradiated seeds showed small comet-length variation depending on dose.
- Taxon
- RiceOryza sativae1
- Treatment
- gamma-irradiatede0
- Outcome
- variations in the comet lengthe0
- Direction
- smalle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cells of gamma-irradiated seed showed small variations in the comet length, and which were depending on radiation dose.”
e1“Rice seeds”
Claim · model-written summary
At 1kGy, gamma irradiated seed cells had shorter comets than electron damaged cells.
- Taxon
- RiceOryza sativae1
- Treatment
- gamma-irradiated seeds at 1kGye0
- Outcome
- comete0
- Direction
- shortere0
- Comparator
- damaged cells with soft electrone0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The individual cells of gamma-irradiated seeds at 1kGy showed shorter comet than the damaged cells with soft electron”
e1“Rice seeds”
Claim · model-written summary
Gamma irradiation at 1-5kGy prevented seed shoots and roots.
- Taxon
- RiceOryza sativae1
- Treatment
- gamma rays (1-5kGy)e0
- Outcome
- did not shoot nor roote0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed treated with gamma rays (1-5kGy) did not shoot nor root.”
e1“Rice seeds”
The same questions in other papers
electron · organism · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.