Abstract
The changes in the activities of enzymes and proteins that may be involved in localized reactions of cucumber cotyledons to tobacco mosaic virus (TMV) were investigated. The peroxidase (PO) and ribonuclease (RNase) activity of the cotyledons increased with the progress of TMV infection, while no increase was observed in plants systemically infected with cucumber green mottle mosaic virus (CGMMV). A marked increase in PO and RNase activity was also observed in cotyledons in which TMV localization had been weakened by actinomycin D (AMD) treatment. In this case the profiles of perox idase-acidic isozymes and RNase-acidic isozymes did not change. Little polyphenoloxidase activity was detected in TMV-infected cucumber cotyledons. These enzymes thus do not play an active role in the localization process of TMV. When cucumber cotyledons were infected with tobacco necrotic virus or treated with salicylic acid, two pathogenesis-related proteins (PRps) detectable by electrophoresis were induced in the cotyledons. These two proteins were not detected in the cucumber cotyledons infected with TMV. When the two PRps were induced by salicylic acid treatment of cucumber cotyledons, little change was observed in the amount of TMV and in the diameter of starch-lesions. Therefore the two PRps may not be related to the localization of virus. New induced protein bands (82 kDa and 86 kDa) were detected 24 hr after TMV-inoculation. These proteins disappeared when TMV localization was weakened by AMD treatment or by prior inoculation with zucchini yellow moxaic virus (ZYMV). Thus these proteins seem to be directly related to TMV localization in cucumber cotyledons.
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Claims 14
Claim · model-written summary
Peroxidase activity rose as TMV infection progressed in cucumber cotyledons.
- Taxon
- cucumber
Cucumis sativust0 - Interactor
- TMV
Tobacco mosaic viruse0pathogen - Gene
- peroxidase (PO)e0
- Outcome
- activity of the cotyledonse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The peroxidase (PO) and ribonuclease (RNase) activity of the cotyledons increased with the progress of TMV infection, while no increase was observed in plants systemically infected with cucumber green mottle mosaic virus (CGMMV).”
t0“Cucumber Cotyledons”
Claim · model-written summary
Ribonuclease activity rose as TMV infection progressed in cucumber cotyledons.
- Taxon
- cucumberCucumis sativust0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Gene
- ribonuclease (RNase)e0
- Outcome
- ribonuclease (RNase) activitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The peroxidase (PO) and ribonuclease (RNase) activity of the cotyledons increased with the progress of TMV infection, while no increase was observed in plants systemically infected with cucumber green mottle mosaic virus (CGMMV).”
t0“Cucumber Cotyledons”
Claim · model-written summary
PO and RNase activity did not increase with systemic CGMMV infection.
- Taxon
- cucumberCucumis sativust0
- Interactor
- cucumber green mottle mosaic virus (CGMMV)Cucumber green mottle mosaic viruse0pathogen
- Outcome
- no increasee0
- Direction
- no increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while no increase was observed in plants systemically infected with cucumber green mottle mosaic virus (CGMMV).”
t0“Cucumber Cotyledons”
Claim · model-written summary
PO and RNase activity increased markedly when actinomycin D weakened TMV localization.
- Taxon
- cucumberCucumis sativust0
- Treatment
- actinomycin D (AMD) treatmente0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Outcome
- PO and RNase activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A marked increase in PO and RNase activity was also observed in cotyledons in which TMV localization had been weakened by actinomycin D (AMD) treatment.”
t0“Cucumber Cotyledons”
Claim · model-written summary
Acidic PO and RNase isozyme profiles did not change under actinomycin D treatment.
- Taxon
- cucumberCucumis sativust0
- Treatment
- actinomycin D (AMD) treatmente1
- Outcome
- profiles of perox idase-acidic isozymes and RNase-acidic isozymese0
- Direction
- did not changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In this case the profiles of perox idase-acidic isozymes and RNase-acidic isozymes did not change.”
t0“Cucumber Cotyledons”
e1“actinomycin D (AMD) treatment”
Claim · model-written summary
TMV-infected cucumber cotyledons had little polyphenoloxidase activity.
- Taxon
- cucumberCucumis sativust0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Outcome
- polyphenoloxidase activitye0
- Direction
- Littlee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Little polyphenoloxidase activity was detected in TMV-infected cucumber cotyledons.”
t0“Cucumber Cotyledons”
Claim · model-written summary
Tobacco necrotic virus induced two pathogenesis-related proteins in cucumber cotyledons.
- Taxon
- cucumberCucumis sativust0
- Treatment
- infected with tobacco necrotic viruse0
- Interactor
- tobacco necrotic viruse0pathogen
- Outcome
- two pathogenesis-related proteins (PRps)e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When cucumber cotyledons were infected with tobacco necrotic virus or treated with salicylic acid, two pathogenesis-related proteins (PRps) detectable by electrophoresis were induced in the cotyledons.”
t0“Cucumber Cotyledons”
Claim · model-written summary
Salicylic acid induced two pathogenesis-related proteins in cucumber cotyledons.
- Taxon
- cucumberCucumis sativust0
- Treatment
- salicylic acide0
- Outcome
- two pathogenesis-related proteins (PRps)e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When cucumber cotyledons were infected with tobacco necrotic virus or treated with salicylic acid, two pathogenesis-related proteins (PRps) detectable by electrophoresis were induced in the cotyledons.”
t0“Cucumber Cotyledons”
Claim · model-written summary
The two PRps were undetected in TMV-infected cucumber cotyledons.
- Taxon
- cucumberCucumis sativust0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Outcome
- two proteins were not detectede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These two proteins were not detected in the cucumber cotyledons infected with TMV.”
t0“Cucumber Cotyledons”
Claim · model-written summary
Salicylic acid induction of PRps changed TMV amount little.
- Taxon
- cucumberCucumis sativust0
- Treatment
- salicylic acid treatmente0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Outcome
- amount of TMVe0
- Direction
- little changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the two PRps were induced by salicylic acid treatment of cucumber cotyledons, little change was observed in the amount of TMV and in the diameter of starch-lesions.”
t0“Cucumber Cotyledons”
Claim · model-written summary
Salicylic acid induction of PRps changed starch-lesion diameter little.
- Taxon
- cucumberCucumis sativust0
- Treatment
- salicylic acid treatmente0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Outcome
- diameter of starch-lesionse0
- Direction
- little changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the two PRps were induced by salicylic acid treatment of cucumber cotyledons, little change was observed in the amount of TMV and in the diameter of starch-lesions.”
t0“Cucumber Cotyledons”
Claim · model-written summary
Protein bands of 82 and 86 kDa appeared 24 hr after TMV inoculation.
- Taxon
- cucumberCucumis sativust0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Outcome
- New induced protein bandse0
- Value
- 82 kDa and 86 kDae0
- Qualifier
- 24 hr after TMV-inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“New induced protein bands (82 kDa and 86 kDa) were detected 24 hr after TMV-inoculation.”
t0“Cucumber Cotyledons”
Claim · model-written summary
TMV-induced proteins disappeared when AMD weakened TMV localization.
- Taxon
- cucumberCucumis sativust0
- Treatment
- AMD treatmente0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Outcome
- These proteinse0
- Direction
- disappearede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These proteins disappeared when TMV localization was weakened by AMD treatment or by prior inoculation with zucchini yellow moxaic virus (ZYMV).”
t0“Cucumber Cotyledons”
Claim · model-written summary
TMV-induced proteins disappeared after prior ZYMV inoculation.
- Taxon
- cucumberCucumis sativust0
- Treatment
- prior inoculation with zucchini yellow moxaic virus (ZYMV)e0
- Interactor
- zucchini yellow moxaic virus (ZYMV)
Zucchini yellow mosaic viruse0pathogen - Outcome
- These proteinse0
- Direction
- disappearede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These proteins disappeared when TMV localization was weakened by AMD treatment or by prior inoculation with zucchini yellow moxaic virus (ZYMV).”
t0“Cucumber Cotyledons”
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.