Abstract
Basic helix-loop-helix (bHLH) transcription factors constitute one of the largest families in plants and are known to be involved in various developmental processes and stress tolerance. We report the characterization of a stress responsive bHLH transcription factor from stress adapted species finger millet which is homologous to OsbHLH57 and designated as EcbHLH57. The full length sequence of EcbHLH57 consisted of 256 amino acids with a conserved bHLH domain followed by leucine repeats. In finger millet, EcbHLH57 transcripts were induced by ABA, NaCl, PEG, methyl viologen (MV) treatments and drought stress. Overexpression of EcbHLH57 in tobacco significantly increased the tolerance to salinity and drought stress with improved root growth. Transgenic plants showed higher photosynthetic rate and stomatal conductance under drought stress that resulted in higher biomass. Under long-term salinity stress, the transgenic plants accumulated higher seed weight/pod and pod number. The transgenic plants were also tolerant to oxidative stress and showed less accumulation of H202 and MDA levels. The overexpression of EcbHLH57 enhanced the expression of stress responsive genes such as LEA14, rd29A, rd29B, SOD, APX, ADH1, HSP70 and also PP2C and hence improved tolerance to diverse stresses.
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Claims 9
Claim · model-written summary
EcbHLH57 encodes 256 amino acids with a conserved bHLH domain and leucine repeats.
- Taxon
- Eleusine coracana L.
Eleusine coracanat0 - Gene
- EcbHLH57e0
- Outcome
- full length sequencee0
- Value
- 256 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full length sequence of EcbHLH57 consisted of 256 amino acids with a conserved bHLH domain followed by leucine repeats”
t0“Eleusine coracana L.”
Claim · model-written summary
ABA, NaCl, PEG, methyl viologen and drought induced EcbHLH57 transcripts in finger millet.
- Taxon
- finger milletEleusine coracanae0
- Treatment
- ABA, NaCl, PEG, methyl viologen (MV) treatments and drought stresse0
- Gene
- EcbHLH57e0
- Outcome
- EcbHLH57 transcriptse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In finger millet, EcbHLH57 transcripts were induced by ABA, NaCl, PEG, methyl viologen (MV) treatments and drought stress”
Claim · model-written summary
EcbHLH57 overexpression increased tobacco tolerance to salinity and drought with improved root growth.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Overexpression of EcbHLH57e0
- Gene
- EcbHLH57e0
- Outcome
- tolerance to salinity and drought stress with improved root growthe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of EcbHLH57 in tobacco significantly increased the tolerance to salinity and drought stress with improved root growth”
Claim · model-written summary
Transgenic tobacco had higher photosynthetic rate and stomatal conductance under drought.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- Transgenic plantse0
- Outcome
- photosynthetic rate and stomatal conductancee0
- Direction
- highere0
- Qualifier
- under drought stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plants showed higher photosynthetic rate and stomatal conductance under drought stress”
t0“in Tobacco”
Claim · model-written summary
Transgenic tobacco had higher biomass under drought.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- biomasse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under drought stress that resulted in higher biomass”
t0“in Tobacco”
Claim · model-written summary
Transgenic tobacco accumulated higher seed weight per pod and pod number under long-term salinity.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- seed weight/pod and pod numbere0
- Direction
- highere0
- Qualifier
- Under long-term salinity stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under long-term salinity stress, the transgenic plants accumulated higher seed weight/pod and pod number”
t0“in Tobacco”
Claim · model-written summary
Transgenic tobacco tolerated oxidative stress.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- tolerant to oxidative stresse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants were also tolerant to oxidative stress”
t0“in Tobacco”
Claim · model-written summary
Transgenic tobacco accumulated less H202 and MDA.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- H202 and MDA levelse0
- Direction
- lesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“showed less accumulation of H202 and MDA levels”
t0“in Tobacco”
Claim · model-written summary
EcbHLH57 overexpression enhanced expression of stress responsive genes and PP2C.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- overexpression of EcbHLH57e0
- Gene
- EcbHLH57e0
- Outcome
- expression of stress responsive genes such as LEA14, rd29A, rd29B, SOD, APX, ADH1, HSP70 and also PP2Ce0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of EcbHLH57 enhanced the expression of stress responsive genes such as LEA14, rd29A, rd29B, SOD, APX, ADH1, HSP70 and also PP2C”
t0“in Tobacco”
The same questions in other papers
drought · molecular: expression · Eleusine coracana (finger millet)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.