Paper · 2016 · Saudi Journal of Biological Sciences

The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato ( Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles

Hesham F. Alharby; Ehab M.R. Metwali; Michael P. Fuller; et al.

7
claims
15
verbatim quotes
79
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

Five cultivars of tomato having different levels of salt stress tolerance were exposed to different treatments of NaCl (0, 3 and 6 g L−1) and ZnO-NPs (0, 15 and 30 mg L−1). Treatments with NaCl at both 3 and 6 g L−1 suppressed the mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) genes in all cultivars while plants treated with ZnO-NPs in the presence of NaCl, showed increments in the mRNA expression levels. This indicated that ZnO-NPs had a positive response on plant metabolism under salt stress. Superior expression levels of mRNA were observed in the salt tolerant cultivars, Sandpoint and Edkawy while the lowest level was detected in the salt sensitive cultivar, Anna Aasa. SDS–PAGE showed clear differences in patterns of protein expression among the cultivars. A negative protein marker for salt sensitivity and ZnO-NPs was detected in cv. Anna Aasa at a molecular weight of 19.162 kDa, while the tolerant cultivar Edkawy had two positive markers at molecular weights of 74.991 and 79.735 kDa.

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Claims 7

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

NaCl suppressed SOD and GPX mRNA in all five cultivars at both doses.

Treatment
NaCl at both 3 and 6 g L−1e0
Gene
superoxide dismutase (SOD) and glutathione peroxidase (GPX) genese0
Outcome
mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) genese0
Direction
suppressede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Treatments with NaCl at both 3 and 6 g L−1 suppressed the mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) genes in all cultivars”

✓ located verbatim in the source · abstract, characters 173–328 · show in text ↑
e1

“Five cultivars of tomato”

✓ located verbatim in the source · abstract, characters 0–24 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

ZnO nanoparticles with NaCl increased the mRNA expression levels.

Treatment
ZnO-NPs in the presence of NaCle0
Outcome
mRNA expression levelse0
Direction
incrementse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“plants treated with ZnO-NPs in the presence of NaCl, showed increments in the mRNA expression levels”

✓ located verbatim in the source · abstract, characters 335–435 · show in text ↑
e1

“Five cultivars of tomato”

✓ located verbatim in the source · abstract, characters 0–24 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Sandpoint and Edkawy had superior mRNA expression levels.

Material
Sandpoint and Edkawye0
Outcome
expression levels of mRNAe0
Direction
Superiore0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Superior expression levels of mRNA were observed in the salt tolerant cultivars, Sandpoint and Edkawy”

✓ located verbatim in the source · abstract, characters 528–629 · show in text ↑
e1

“Five cultivars of tomato”

✓ located verbatim in the source · abstract, characters 0–24 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Anna Aasa had the lowest mRNA expression level.

Material
Anna Aasae0
Outcome
levele0
Direction
loweste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the lowest level was detected in the salt sensitive cultivar, Anna Aasa”

✓ located verbatim in the source · abstract, characters 636–707 · show in text ↑
e1

“Superior expression levels of mRNA were observed in the salt tolerant cultivars, Sandpoint and Edkawy”

✓ located verbatim in the source · abstract, characters 528–629 · show in text ↑
e2

“Five cultivars of tomato”

✓ located verbatim in the source · abstract, characters 0–24 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Protein expression patterns differed among the tomato cultivars.

Outcome
patterns of protein expressione0
Direction
differencese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“SDS–PAGE showed clear differences in patterns of protein expression among the cultivars”

✓ located verbatim in the source · abstract, characters 709–796 · show in text ↑
e1

“Five cultivars of tomato”

✓ located verbatim in the source · abstract, characters 0–24 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Anna Aasa had a negative protein marker at 19.162 kDa.

Material
Anna Aasae0
Outcome
negative protein markere0
Value
19.162 kDae0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A negative protein marker for salt sensitivity and ZnO-NPs was detected in cv. Anna Aasa at a molecular weight of 19.162 kDa”

✓ located verbatim in the source · abstract, characters 798–922 · show in text ↑
e1

“Five cultivars of tomato”

✓ located verbatim in the source · abstract, characters 0–24 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Edkawy had two positive markers at 74.991 and 79.735 kDa.

Material
Edkawye0
Outcome
two positive markerse0
Value
74.991 and 79.735 kDae0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the tolerant cultivar Edkawy had two positive markers at molecular weights of 74.991 and 79.735 kDa”

✓ located verbatim in the source · abstract, characters 930–1029 · show in text ↑
e1

“Five cultivars of tomato”

✓ located verbatim in the source · abstract, characters 0–24 · show in text ↑

sodium chloride · physiology: antioxidant enzymes · Solanum lycopersicum (tomato)Results differ

12 papers: 6 positive, 6 negative or no effect

5 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

zinc oxide · molecular: expression · Solanum lycopersicum (tomato)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

nanoparticle · molecular: expression · Solanum lycopersicum (tomato)Results agree

18 papers: 18 positive, 0 negative or no effect

17 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

14 papers more in this group

See all 18 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.