Abstract
The importance of the nitrate (NO3-) transporter for yield and nitrogen-use efficiency (NUE) in rice was previously demonstrated using map-based cloning. In this study, we enhanced the expression of the OsNRT2.1 gene, which encodes a high-affinity NO3- transporter, using a ubiquitin (Ubi) promoter and the NO3--inducible promoter of the OsNAR2.1 gene to drive OsNRT2.1 expression in transgenic rice plants. Transgenic lines expressing pUbi:OsNRT2.1 or pOsNAR2.1:OsNRT2.1 constructs exhibited the increased total biomass including yields of approximately 21% and 38% compared with wild-type (WT) plants. The agricultural NUE (ANUE) of the pUbi:OsNRT2.1 lines decreased to 83% of that of the WT plants, while the ANUE of the pOsNAR2.1:OsNRT2.1 lines increased to 128% of that of the WT plants. The dry matter transfer into grain decreased by 68% in the pUbi:OsNRT2.1 lines and increased by 46% in the pOsNAR2.1:OsNRT2.1 lines relative to the WT. The expression of OsNRT2.1 in shoot and grain showed that Ubi enhanced OsNRT2.1 expression by 7.5-fold averagely and OsNAR2.1 promoters increased by about 80% higher than the WT. Interestingly, we found that the OsNAR2.1 was expressed higher in all the organs of pUbi:OsNRT2.1 lines; however, for pOsNAR2.1:OsNRT2.1 lines, OsNAR2.1 expression was only increased in root, leaf sheaths and internodes. We show that increased expression of OsNRT2.1, especially driven by OsNAR2.1 promoter, can improve the yield and NUE in rice.
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Claims 11
Claim · model-written summary
pUbi:OsNRT2.1 transgenic lines increased total biomass including yield by approximately 21% compared with wild-type plants.
- Taxon
- rice
Oryza sativat0 - Material
- Transgenic linese1
- Treatment
- pUbi:OsNRT2.1e1
- Gene
- OsNRT2.1e1
- Outcome
- total biomass including yieldse1
- Direction
- increasede1
- Value
- 21%e1
- Comparator
- wild-type (WT) plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“efficiency of rice”
e1“Transgenic lines expressing pUbi:OsNRT2.1 or pOsNAR2.1:OsNRT2.1 constructs exhibited the increased total biomass including yields of approximately 21% and 38% compared with wild-type (WT) plants”
Claim · model-written summary
pOsNAR2.1:OsNRT2.1 transgenic lines increased total biomass including yield by approximately 38% compared with wild-type plants.
- Taxon
- riceOryza sativat0
- Material
- Transgenic linese1
- Treatment
- pOsNAR2.1:OsNRT2.1e1
- Gene
- OsNRT2.1e1
- Outcome
- total biomass including yieldse1
- Direction
- increasede1
- Value
- 38%e1
- Comparator
- wild-type (WT) plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“efficiency of rice”
e1“Transgenic lines expressing pUbi:OsNRT2.1 or pOsNAR2.1:OsNRT2.1 constructs exhibited the increased total biomass including yields of approximately 21% and 38% compared with wild-type (WT) plants”
Claim · model-written summary
Agricultural NUE of pUbi:OsNRT2.1 lines decreased to 83% of wild-type.
- Taxon
- riceOryza sativat0
- Treatment
- pUbi:OsNRT2.1e2
- Gene
- OsNRT2.1e2
- Outcome
- agricultural NUE (ANUE)e2
- Direction
- decreasede2
- Value
- 83%e2
- Comparator
- WT plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“efficiency of rice”
e2“The agricultural NUE (ANUE) of the pUbi:OsNRT2.1 lines decreased to 83% of that of the WT plants”
Claim · model-written summary
Agricultural NUE of pOsNAR2.1:OsNRT2.1 lines increased to 128% of wild-type.
- Taxon
- riceOryza sativat0
- Treatment
- pOsNAR2.1:OsNRT2.1e3
- Gene
- OsNRT2.1e3
- Outcome
- ANUEe3
- Direction
- increasede3
- Value
- 128%e3
- Comparator
- WT plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“efficiency of rice”
e3“the ANUE of the pOsNAR2.1:OsNRT2.1 lines increased to 128% of that of the WT plants”
Claim · model-written summary
Dry matter transfer into grain decreased by 68% in pUbi:OsNRT2.1 lines relative to wild-type.
- Taxon
- riceOryza sativat0
- Treatment
- pUbi:OsNRT2.1e4
- Gene
- OsNRT2.1e4
- Outcome
- dry matter transfer into graine4
- Direction
- decreasede4
- Value
- 68%e4
- Comparator
- WTe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“efficiency of rice”
e4“The dry matter transfer into grain decreased by 68% in the pUbi:OsNRT2.1 lines and increased by 46% in the pOsNAR2.1:OsNRT2.1 lines relative to the WT”
Claim · model-written summary
Dry matter transfer into grain increased by 46% in pOsNAR2.1:OsNRT2.1 lines relative to wild-type.
- Taxon
- riceOryza sativat0
- Treatment
- pOsNAR2.1:OsNRT2.1e4
- Gene
- OsNRT2.1e4
- Outcome
- dry matter transfer into graine4
- Direction
- increasede4
- Value
- 46%e4
- Comparator
- WTe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“efficiency of rice”
e4“The dry matter transfer into grain decreased by 68% in the pUbi:OsNRT2.1 lines and increased by 46% in the pOsNAR2.1:OsNRT2.1 lines relative to the WT”
Claim · model-written summary
OsNAR2.1 was expressed higher in all organs of pUbi:OsNRT2.1 lines.
- Taxon
- riceOryza sativat0
- Organ / stage
- all the organse6
- Treatment
- pUbi:OsNRT2.1e6
- Gene
- OsNAR2.1e6
- Outcome
- OsNAR2.1 was expressede6
- Direction
- highere6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“efficiency of rice”
e6“Interestingly, we found that the OsNAR2.1 was expressed higher in all the organs of pUbi:OsNRT2.1 lines”
Claim · model-written summary
In pOsNAR2.1:OsNRT2.1 lines, OsNAR2.1 expression was increased only in root, leaf sheaths and internodes.
- Taxon
- riceOryza sativat0
- Organ / stage
- root, leaf sheaths and internodese7
- Treatment
- pOsNAR2.1:OsNRT2.1e7
- Gene
- OsNAR2.1e7
- Outcome
- OsNAR2.1 expressione7
- Direction
- increasede7
- Qualifier
- onlye7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“efficiency of rice”
e7“for pOsNAR2.1:OsNRT2.1 lines, OsNAR2.1 expression was only increased in root, leaf sheaths and internodes”
Claim · model-written summary
Ubi enhanced OsNRT2.1 expression in shoot and grain by 7.5-fold on average.
- Taxon
- riceOryza sativat0
- Organ / stage
- shoot and graine5
- Treatment
- Ubie5
- Gene
- OsNRT2.1e5
- Outcome
- OsNRT2.1 expressione5
- Direction
- enhancede5
- Value
- 7.5-folde5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“efficiency of rice”
e5“The expression of OsNRT2.1 in shoot and grain showed that Ubi enhanced OsNRT2.1 expression by 7.5-fold averagely and OsNAR2.1 promoters increased by about 80% higher than the WT”
Claim · model-written summary
OsNAR2.1 promoters increased OsNRT2.1 expression in shoot and grain by about 80% compared with wild-type.
- Taxon
- riceOryza sativat0
- Organ / stage
- shoot and graine5
- Treatment
- OsNAR2.1 promoterse5
- Gene
- OsNRT2.1e5
- Outcome
- expression of OsNRT2.1e5
- Direction
- increasede5
- Value
- 80%e5
- Comparator
- WTe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“efficiency of rice”
e5“The expression of OsNRT2.1 in shoot and grain showed that Ubi enhanced OsNRT2.1 expression by 7.5-fold averagely and OsNAR2.1 promoters increased by about 80% higher than the WT”
Claim · model-written summary
Increased OsNRT2.1 expression, especially driven by the OsNAR2.1 promoter, can improve yield and NUE in rice.
- Taxon
- riceOryza sativat0
- Treatment
- increased expression of OsNRT2.1, especially driven by OsNAR2.1 promotere0
- Gene
- OsNRT2.1e0
- Outcome
- yield and NUEe0
- Direction
- improvee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“efficiency of rice”
e0“We show that increased expression of OsNRT2.1, especially driven by OsNAR2.1 promoter, can improve the yield and NUE in rice”
The same questions in other papers
transgenic · nutrient: efficiency · Oryza sativa (rice)Results agree
4 papers in the same direction as this paper
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- positive
- positive
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