Abstract
Cellular pH homeostasis is fundamental for life, and all cells adapt to maintain this balance. In plants, the chemical form of nitrogen supply, nitrate and ammonium, is one of the cellular pH dominators. We report that the rice nitrate transporter OsNRT2.3 is transcribed into two spliced isoforms with a natural variation in expression ratio. One splice form, OsNRT2.3b is located on the plasma membrane, is expressed mainly in the phloem, and has a regulatory motif on the cytosolic side that acts to switch nitrate transport activity on or off by a pH-sensing mechanism. High OsNRT2.3b expression in rice enhances the pH-buffering capacity of the plant, increasing N, Fe, and P uptake. In field trials, increased expression of OsNRT2.3b improved grain yield and nitrogen use efficiency (NUE) by 40%. These results indicate that pH sensing by the rice nitrate transporter OsNRT2.3b is important for plant adaption to varied N supply forms and can provide a target for improving NUE.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Rice OsNRT2.3 has two splice isoforms with natural variation in expression ratio.
- Taxon
- rice
Oryza sativae0 - Gene
- OsNRT2.3e0
- Outcome
- two spliced isoformse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice nitrate transporter OsNRT2.3 is transcribed into two spliced isoforms with a natural variation in expression ratio”
Claim · model-written summary
OsNRT2.3b is on the plasma membrane and expressed mainly in phloem.
Not reportedTaxon
Verbatim
e0“OsNRT2.3b is located on the plasma membrane, is expressed mainly in the phloem”
Claim · model-written summary
A cytosolic regulatory motif switches OsNRT2.3b nitrate transport through pH sensing.
Not reportedTaxon
Verbatim
e0“has a regulatory motif on the cytosolic side that acts to switch nitrate transport activity on or off by a pH-sensing mechanism”
Claim · model-written summary
High OsNRT2.3b expression enhances rice pH buffering capacity.
- Taxon
- riceOryza sativae0
- Treatment
- High OsNRT2.3b expressione0
- Gene
- OsNRT2.3be0
- Outcome
- pH-buffering capacitye0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High OsNRT2.3b expression in rice enhances the pH-buffering capacity of the plant”
Claim · model-written summary
High OsNRT2.3b expression increases rice N, Fe and P uptake.
- Taxon
- riceOryza sativae0
- Treatment
- High OsNRT2.3b expressione0
- Gene
- OsNRT2.3be0
- Outcome
- N, Fe, and P uptakee0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High OsNRT2.3b expression in rice enhances the pH-buffering capacity of the plant, increasing N, Fe, and P uptake”
Claim · model-written summary
In field trials, increased OsNRT2.3b expression improved grain yield by 40%.
- Treatment
- increased expression of OsNRT2.3be0
- Outcome
- grain yielde0
- Direction
- improvede0
- Value
- 40%e0
- Setting
- field trialse0
Not reportedTaxonComparator
Verbatim
e0“In field trials, increased expression of OsNRT2.3b improved grain yield and nitrogen use efficiency (NUE) by 40%”
Claim · model-written summary
In field trials, increased OsNRT2.3b expression improved NUE by 40%.
- Treatment
- increased expression of OsNRT2.3be0
- Outcome
- nitrogen use efficiency (NUE)e0
- Direction
- improvede0
- Value
- 40%e0
- Setting
- field trialse0
Not reportedTaxonComparator
Verbatim
e0“In field trials, increased expression of OsNRT2.3b improved grain yield and nitrogen use efficiency (NUE) by 40%”
The same questions in other papers
transgenic · yield · Oryza sativa (rice)Positive and negative results
80 papers in the same direction as this paper · 18 in the opposite direction · 3 reporting no difference from an alternative
- positive
- no effect
- negative
98 papers more in this group
See all 102 papers →transgenic · nutrient: efficiency · Oryza sativa (rice)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.