Abstract
Determining the suitable and optimized Minimal Inhibitory Concentration (MIC) of the selectable agents is critical to successfully recover stably transformed plants. In this study, the MIC of five different antibiotics and one herbicide was determined for their inhibitory effect on the growth of a recalcitrant rice cultivar, MR219 using rice shoot apices as target tissue. Antibiotics such as kanamycin, paromomycin and neomycin did not inhibit the shoot apices growth while geneticin (G-418) and hygromycin showed complete growth inhibition at 400 and 25 mg LG 1 , respectively. Similarly, the herbicide basta indicated that 9 mg LG 1 was sufficient to cause growth inhibition. To conclude, hygromycin and basta were suggested to the suitable selectable agent to select transformed rice shoot apices.
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Claims 5
Claim · model-written summary
Geneticin (G-418) completely inhibited growth of rice shoot apices at 400 mg LG 1.
- Taxon
- rice
Oryza sativae0 - Material
- MR219e0
- Organ / stage
- shoot apicese0
- Treatment
- geneticin (G-418)e1
- Outcome
- growth inhibitione1
- Value
- 400e1
- Qualifier
- mg LG 1e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“recalcitrant rice cultivar, MR219 using rice shoot apices as target tissue”
e1“geneticin (G-418) and hygromycin showed complete growth inhibition at 400 and 25 mg LG 1 , respectively”
Claim · model-written summary
Hygromycin completely inhibited growth of rice shoot apices at 25 mg LG 1.
- Taxon
- riceOryza sativae0
- Material
- MR219e0
- Organ / stage
- shoot apicese0
- Treatment
- hygromycine1
- Outcome
- growth inhibitione1
- Value
- 25e1
- Qualifier
- mg LG 1e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“recalcitrant rice cultivar, MR219 using rice shoot apices as target tissue”
e1“geneticin (G-418) and hygromycin showed complete growth inhibition at 400 and 25 mg LG 1 , respectively”
Claim · model-written summary
Kanamycin, paromomycin and neomycin did not inhibit growth of rice shoot apices.
- Taxon
- riceOryza sativae0
- Material
- MR219e0
- Organ / stage
- shoot apicese1
- Treatment
- kanamycin, paromomycin and neomycine1
- Outcome
- shoot apices growthe1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“recalcitrant rice cultivar, MR219 using rice shoot apices as target tissue”
e1“Antibiotics such as kanamycin, paromomycin and neomycin did not inhibit the shoot apices growth”
Claim · model-written summary
Basta at 9 mg LG 1 was sufficient to cause growth inhibition of rice shoot apices.
- Taxon
- riceOryza sativae0
- Material
- MR219e0
- Organ / stage
- shoot apicese0
- Treatment
- bastae1
- Outcome
- growth inhibitione1
- Value
- 9e1
- Qualifier
- mg LG 1e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“recalcitrant rice cultivar, MR219 using rice shoot apices as target tissue”
e1“the herbicide basta indicated that 9 mg LG 1 was sufficient to cause growth inhibition”
Claim · model-written summary
Hygromycin and basta are suggested as suitable selectable agents to select transformed rice shoot apices.
- Taxon
- riceOryza sativae0
- Organ / stage
- shoot apicese0
- Treatment
- hygromycin and bastae0
- Outcome
- suitable selectable agente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hygromycin and basta were suggested to the suitable selectable agent to select transformed rice shoot apices”
The same questions in other papers
neomycin · growth · Oryza sativa (rice)Results differ
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
hygromycin · disease · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.