Abstract
Immature embryos of four indica rice varieties, Longtefu B ?Xieqingzao B?Ⅱ 32 B and Zhenshan 97 B, were used to induce calli, these calli were infected and transformed by A.tumefacien strain EHA105 harboring Ti plasmid p13W4 carrying waxy gene. After cocultivation and two times 25 50mg/L hygromycin resistant selections, 20.1% 27.1% resistant calli still survived, and the transgenic calli frequencies were no difference. These resistant calli were transferred to differentiation medium, and the differentiation frequencies of them were different, Longtefu B 21.2%, Xieqingzao B 12.0%,Ⅱ 32 B 15.1% and Zhenshan B 19.6%,respectively. But the green plant frequency was significantly different: 8.8% resistant calli of Longtefu B generated green plants, but no green plant was obtained in Zhenshan 97 B. All of plants were detected by GUS analysis and PCR amplification, the results showed that the anti Waxy gene had been integrated into these plants. The transgenic plants were normally fertile,and segregation of GUS active was observed among T0 seeds. The effect of hygromycin on calli was tested, no hygromycin differentiation medium can improve the plantlet frequency of resistant calli, but the reliability of transgenic plant decreased.
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Claims 14
Claim · model-written summary
Hygromycin selection left 20.1% to 27.1% resistant calli surviving.
- Taxon
- indica rice
Oryza sativa Indica Groupt0 - Outcome
- resistant calli still survivede0
- Value
- 20.1% 27.1%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“20.1% 27.1% resistant calli still survived”
t0“indica rice”
Claim · model-written summary
Transgenic callus frequencies did not differ among varieties.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Outcome
- transgenic calli frequenciese0
- Direction
- no differencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenic calli frequencies were no difference”
t0“indica rice”
Claim · model-written summary
Longtefu B differentiation frequency was 21.2%.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Longtefu Be0
- Outcome
- differentiation frequenciese1
- Value
- 21.2%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Longtefu B 21.2%”
e1“the differentiation frequencies of them were different”
t0“indica rice”
Claim · model-written summary
Xieqingzao B differentiation frequency was 12.0%.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Xieqingzao Be0
- Outcome
- differentiation frequenciese1
- Value
- 12.0%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xieqingzao B 12.0%”
e1“the differentiation frequencies of them were different”
t0“indica rice”
Claim · model-written summary
Line Ⅱ 32 B differentiation frequency was 15.1%.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Ⅱ 32 Be0
- Outcome
- differentiation frequenciese1
- Value
- 15.1%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ⅱ 32 B 15.1%”
e1“the differentiation frequencies of them were different”
t0“indica rice”
Claim · model-written summary
Zhenshan B differentiation frequency was 19.6%.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Zhenshan Be0
- Outcome
- differentiation frequenciese1
- Value
- 19.6%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zhenshan B 19.6%”
e1“the differentiation frequencies of them were different”
t0“indica rice”
Claim · model-written summary
Green plant frequency differed significantly among varieties.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Outcome
- green plant frequencye0
- Direction
- differente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the green plant frequency was significantly different”
t0“indica rice”
Claim · model-written summary
Longtefu B yielded green plants from 8.8% of resistant calli.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Longtefu Be0
- Outcome
- generated green plantse0
- Value
- 8.8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“8.8% resistant calli of Longtefu B generated green plants”
t0“indica rice”
Claim · model-written summary
Zhenshan 97 B yielded no green plant.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Zhenshan 97 Be0
- Outcome
- no green plant was obtainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no green plant was obtained in Zhenshan 97 B”
t0“indica rice”
Claim · model-written summary
The anti Waxy gene was integrated into regenerated plants.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Gene
- anti Waxy genee0
- Outcome
- integrated into these plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the anti Waxy gene had been integrated into these plants”
t0“indica rice”
Claim · model-written summary
Transgenic plants were normally fertile.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Outcome
- normally fertilee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants were normally fertile”
t0“indica rice”
Claim · model-written summary
GUS activity segregated among T0 seeds.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Organ / stage
- T0 seedse0
- Gene
- GUSe0
- Outcome
- segregation of GUS activee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“segregation of GUS active was observed among T0 seeds”
t0“indica rice”
Claim · model-written summary
Medium without hygromycin improved resistant callus plantlet frequency.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- no hygromycin differentiation mediume0
- Outcome
- plantlet frequency of resistant callie0
- Direction
- improvee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no hygromycin differentiation medium can improve the plantlet frequency of resistant calli”
t0“indica rice”
Claim · model-written summary
Medium without hygromycin decreased reliability of transgenic plants.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- no hygromycin differentiation mediume0
- Outcome
- reliability of transgenic plante0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no hygromycin differentiation medium can improve the plantlet frequency of resistant calli, but the reliability of transgenic plant decreased”
t0“indica rice”
The same questions in other papers
hygromycin · disease · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
in vitro · disease · Oryza sativa (rice)Positive and negative results
12 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.