Abstract
The plant apoplast, which serves as the frontline battleground for long-term host-pathogen interactions, harbors a wealth of disease resistance resources. However, the identification of the disease resistance proteins in the apoplast is relatively lacking. In this study, we identified and characterized the rice secretory protein OsSSP1 (Oryza sativa secretory small protein 1). OsSSP1 can be secreted into the plant apoplast, and either in vitro treatment of recombinant OsSSP1 or overexpression of OsSSP1 in rice could trigger plant immune response. The expression of OsSSP1 is suppressed significantly during Magnaporthe oryzae infection in the susceptible rice variety Taibei 309, and OsSSP1-overexpressing lines all show strong resistance to M. oryzae. Combining the knockout and overexpression results, we found that OsSSP1 positively regulates plant immunity in response to fungal infection. Moreover, the recognition and immune response triggered by OsSSP1 depend on an uncharacterized transmembrane OsSSR1 (secretory small protein receptor 1) and the key co-receptor OsBAK1, since most of the induced immune response and resistance are lost in the absence of OsSSR1 or OsBAK1. Intriguingly, the OsSSP1 protein is relatively stable and can still induce plant resistance after 1 week of storage in the open environment, and exogenous OsSSP1 treatment for a 2-week period did not affect rice yield. Collectively, our study reveals that OsSSP1 can be secreted into the apoplast and percepted by OsSSR1 and OsBAK1 during fungal infection, thereby triggering the immune response to enhance plant resistance to M. oryzae. These findings provide novel resources and potential strategies for crop breeding and disease control.
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Claims 10
Claim · model-written summary
Rice OsSSP1 is secreted into the apoplast.
- Taxon
- rice
Oryza sativat0 - Gene
- OsSSP1e0
- Outcome
- secreted into the plant apoplaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsSSP1 can be secreted into the plant apoplast”
t0“rice”
Claim · model-written summary
Recombinant OsSSP1 treatment triggered a plant immune response.
- Taxon
- riceOryza sativae0
- Treatment
- in vitro treatment of recombinant OsSSP1e0
- Gene
- OsSSP1e0
- Outcome
- plant immune responsee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in vitro treatment of recombinant OsSSP1 or overexpression of OsSSP1 in rice could trigger plant immune response”
Claim · model-written summary
OsSSP1 overexpression triggered a rice immune response.
- Taxon
- riceOryza sativae0
- Treatment
- overexpression of OsSSP1e0
- Gene
- OsSSP1e0
- Outcome
- plant immune responsee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in vitro treatment of recombinant OsSSP1 or overexpression of OsSSP1 in rice could trigger plant immune response”
Claim · model-written summary
Magnaporthe oryzae infection significantly suppressed OsSSP1 expression in susceptible Taibei 309 rice.
- Taxon
- riceOryza sativae0
- Material
- Taibei 309e0
- Interactor
- Magnaporthe oryzae
Pyricularia oryzaee0pathogen - Gene
- OsSSP1e0
- Outcome
- expression of OsSSP1e0
- Direction
- suppressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of OsSSP1 is suppressed significantly during Magnaporthe oryzae infection in the susceptible rice variety Taibei 309”
Claim · model-written summary
OsSSP1-overexpressing rice lines showed strong resistance to M. oryzae.
- Taxon
- riceOryza sativat0
- Treatment
- OsSSP1-overexpressinge0
- Interactor
- M. oryzaePyricularia oryzaee0pathogen
- Gene
- OsSSP1e0
- Outcome
- strong resistance to M. oryzaee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsSSP1-overexpressing lines all show strong resistance to M. oryzae”
t0“rice”
Claim · model-written summary
Knockout and overexpression results indicate that OsSSP1 positively regulates immunity during fungal infection.
- Taxon
- riceOryza sativat0
- Gene
- OsSSP1e0
- Outcome
- plant immunitye0
- Qualifier
- positively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Combining the knockout and overexpression results, we found that OsSSP1 positively regulates plant immunity in response to fungal infection”
t0“rice”
Claim · model-written summary
OsSSP1-induced immunity and resistance were largely lost without OsSSR1.
- Taxon
- riceOryza sativat0
- Treatment
- absence of OsSSR1e0
- Gene
- OsSSR1e0
- Outcome
- induced immune response and resistancee0
- Direction
- loste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most of the induced immune response and resistance are lost in the absence of OsSSR1 or OsBAK1”
t0“rice”
Claim · model-written summary
OsSSP1-induced immunity and resistance were largely lost without OsBAK1.
- Taxon
- riceOryza sativat0
- Treatment
- absence of OsSSR1 or OsBAK1e0
- Gene
- OsBAK1e0
- Outcome
- induced immune response and resistancee0
- Direction
- loste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most of the induced immune response and resistance are lost in the absence of OsSSR1 or OsBAK1”
t0“rice”
Claim · model-written summary
Stored OsSSP1 remained able to induce plant resistance after one week outdoors.
- Taxon
- riceOryza sativat0
- Gene
- OsSSP1e0
- Outcome
- induce plant resistancee0
- Qualifier
- after 1 week of storage in the open environmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsSSP1 protein is relatively stable and can still induce plant resistance after 1 week of storage in the open environment”
t0“rice”
Claim · model-written summary
Two weeks of exogenous OsSSP1 treatment did not affect rice yield.
- Taxon
- riceOryza sativae0
- Treatment
- exogenous OsSSP1 treatment for a 2-week periode0
- Gene
- OsSSP1e0
- Outcome
- rice yielde0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous OsSSP1 treatment for a 2-week period did not affect rice yield”
The same questions in other papers
in vitro · disease · Oryza sativa (rice)Positive and negative results
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