Abstract
We have studied the effects of neomycin, a potent inhibitor of inositol phospholipid-specific phospholipase C (PLC), on the germination of rice seed and the gibberellin-induced expression of alpha-amylase in the aleurone layer and the scutellar tissues. It was shown that, in the absence of exogenous Ca2+, neomycin markedly reduced the germination speed and seedling growth of rice seeds and inhibited the gibberellin-induced expression of alpha-amylase in both secretory tissues. In addition, neomycin decreased the formation of inositol 1,4,5-trisphosphate (IP3) in the gibberellin-treated aleurone layer and the scutellar tissues. However, the inhibitory effects on the germination speed and the expression of alpha-amylase activity were overcome by supplementation of Ca2+. In addition, gibberellin elevated the level of IP3, and ABA prevented the gibberellin-induced formation of IP3, although ABA alone did not alter the IP3 level. The expression of a membrane-bound PLC molecule in rice aleurone layer was shown to be induced by gibberellin, and the gibberellin-induced expression of PLC was markedly delayed by treatment with ABA. These results strongly suggest that the phosphoinositide-Ca2+ signal transduction pathway may play an important role in the gibberellin-induced expression of alpha-amylase molecules closely related to the germination processes of rice seed.
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Claims 12
Claim · model-written summary
Without exogenous calcium, neomycin reduced rice seed germination speed.
- Taxon
- rice
Oryza sativae0 - Treatment
- neomycine0
- Outcome
- germination speede0
- Direction
- reducede0
- Qualifier
- in the absence of exogenous Ca2+e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the absence of exogenous Ca2+, neomycin markedly reduced the germination speed and seedling growth of rice seeds”
Claim · model-written summary
Without exogenous calcium, neomycin reduced rice seedling growth.
- Taxon
- riceOryza sativae0
- Treatment
- neomycine0
- Outcome
- seedling growthe0
- Direction
- reducede0
- Qualifier
- in the absence of exogenous Ca2+e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the absence of exogenous Ca2+, neomycin markedly reduced the germination speed and seedling growth of rice seeds”
Claim · model-written summary
Neomycin inhibited gibberellin induced alpha-amylase expression in both secretory tissues.
- Taxon
- Rice SeedOryza sativat0
- Treatment
- neomycine1
- Gene
- alpha-amylasee0
- Outcome
- gibberellin-induced expression of alpha-amylasee0
- Direction
- inhibitede0
- Qualifier
- both secretory tissuese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibited the gibberellin-induced expression of alpha-amylase in both secretory tissues.”
e1“neomycin markedly reduced”
t0“Rice Seed”
Claim · model-written summary
Neomycin decreased IP3 formation in gibberellin treated aleurone and scutellar tissues.
- Taxon
- Rice SeedOryza sativat0
- Organ / stage
- aleurone layer and the scutellar tissuese0
- Treatment
- neomycine0
- Outcome
- formation of inositol 1,4,5-trisphosphate (IP3)e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“neomycin decreased the formation of inositol 1,4,5-trisphosphate (IP3) in the gibberellin-treated aleurone layer and the scutellar tissues.”
t0“Rice Seed”
Claim · model-written summary
Calcium supplementation overcame neomycin inhibition of germination speed.
- Taxon
- Rice SeedOryza sativat0
- Treatment
- supplementation of Ca2+e0
- Outcome
- germination speede0
- Direction
- overcomee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inhibitory effects on the germination speed and the expression of alpha-amylase activity were overcome by supplementation of Ca2+.”
t0“Rice Seed”
Claim · model-written summary
Calcium supplementation overcame neomycin inhibition of alpha-amylase activity.
- Taxon
- Rice SeedOryza sativat0
- Treatment
- supplementation of Ca2+e0
- Gene
- alpha-amylasee0
- Outcome
- expression of alpha-amylase activitye0
- Direction
- overcomee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inhibitory effects on the germination speed and the expression of alpha-amylase activity were overcome by supplementation of Ca2+.”
t0“Rice Seed”
Claim · model-written summary
Gibberellin elevated IP3 level.
- Taxon
- Rice SeedOryza sativat0
- Treatment
- gibberelline0
- Outcome
- level of IP3e0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gibberellin elevated the level of IP3”
t0“Rice Seed”
Claim · model-written summary
ABA prevented gibberellin induced IP3 formation.
- Taxon
- Rice SeedOryza sativat0
- Treatment
- ABAe0
- Outcome
- gibberellin-induced formation of IP3e0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA prevented the gibberellin-induced formation of IP3”
t0“Rice Seed”
Claim · model-written summary
ABA alone did not alter IP3 level.
- Taxon
- Rice SeedOryza sativat0
- Treatment
- ABA alonee0
- Outcome
- IP3 levele0
- Direction
- did not altere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA alone did not alter the IP3 level.”
t0“Rice Seed”
Claim · model-written summary
Gibberellin induced membrane bound PLC expression in rice aleurone.
- Taxon
- riceOryza sativae0
- Organ / stage
- aleurone layere0
- Treatment
- gibberelline0
- Gene
- PLCe0
- Outcome
- expression of a membrane-bound PLC moleculee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of a membrane-bound PLC molecule in rice aleurone layer was shown to be induced by gibberellin”
Claim · model-written summary
ABA markedly delayed gibberellin induced PLC expression.
- Taxon
- Rice SeedOryza sativat0
- Treatment
- ABAe0
- Gene
- PLCe0
- Outcome
- gibberellin-induced expression of PLCe0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the gibberellin-induced expression of PLC was markedly delayed by treatment with ABA.”
t0“Rice Seed”
Claim · model-written summary
The authors suggest phosphoinositide calcium signaling plays an important role in gibberellin induced alpha-amylase expression and germination.
- Taxon
- riceOryza sativae0
- Gene
- alpha-amylasee0
- Outcome
- gibberellin-induced expression of alpha-amylase moleculese0
- Qualifier
- phosphoinositide-Ca2+ signal transduction pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the phosphoinositide-Ca2+ signal transduction pathway may play an important role in the gibberellin-induced expression of alpha-amylase molecules closely related to the germination processes of rice seed.”
The same questions in other papers
neomycin · growth · Oryza sativa (rice)Results differ
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
neomycin · molecular: expression · Oryza sativa (rice)Positive and negative results
1 in the opposite direction
- positive
calcium(2+) · molecular: expression · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
calcium(2+) · growth · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →neomycin · molecular: metabolites · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.