Paper · 2000 · Plant and Cell Physiology

Evidence for the Involvement of Phospholipase C in the Anaerobic Signal Transduction

Remo Reggiani; Paolo Laoreti

10
claims
17
verbatim quotes
28
citations
1
research areas
✓
Open access

Abstract

Pre-treatment of rice roots for 2 h in aerobic conditions with two phospholipase C (PLC) antagonists, neomycin and compound 48/80 (C48/80), inhibited accumulation of gamma-aminobutyric acid and increased the loss of K+ in the medium during 3 h of anoxia. The presence of Ca2+ and A23187 (Ca2+ ionophore) nullified the effect of PLC inhibitors. Pre-treatment of rice roots with neomycin and C48/80 abolished the anaerobic increase in the concentration of the PLC product inositol 1,4,5-triphosphate. Stimulation of the anaerobic signal transduction pathway with aluminium fluoride (G protein activator) was attenuated by PLC inhibitors. These findings are consistent with the participation of PLC in the anaerobic response.

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Claims 10

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Neomycin inhibited gamma-aminobutyric acid accumulation during anoxia.

Organ / stage
rootse0
Treatment
neomycine0
Gene
phospholipase Ce0
Outcome
accumulation of gamma-aminobutyric acide0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Pre-treatment of rice roots for 2 h in aerobic conditions with two phospholipase C (PLC) antagonists, neomycin and compound 48/80 (C48/80), inhibited accumulation of gamma-aminobutyric acid”

✓ located verbatim in the source · abstract, characters 0–189 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Compound 48/80 inhibited gamma-aminobutyric acid accumulation during anoxia.

Treatment
compound 48/80e0
Outcome
accumulation of gamma-aminobutyric acide0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“neomycin and compound 48/80 (C48/80), inhibited accumulation of gamma-aminobutyric acid”

✓ located verbatim in the source · abstract, characters 102–189 · show in text ↑
t0

“Pre-treatment of rice roots for 2 h in aerobic conditions”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Neomycin increased K+ loss into the medium during anoxia.

Treatment
neomycine0
Outcome
loss of K+ in the mediume0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“neomycin and compound 48/80 (C48/80), inhibited accumulation of gamma-aminobutyric acid and increased the loss of K+ in the medium during 3 h of anoxia”

✓ located verbatim in the source · abstract, characters 102–253 · show in text ↑
t0

“Pre-treatment of rice roots for 2 h in aerobic conditions”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Compound 48/80 increased K+ loss into the medium during anoxia.

Treatment
compound 48/80e0
Outcome
loss of K+ in the mediume0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“compound 48/80 (C48/80), inhibited accumulation of gamma-aminobutyric acid and increased the loss of K+ in the medium during 3 h of anoxia”

✓ located verbatim in the source · abstract, characters 115–253 · show in text ↑
t0

“Pre-treatment of rice roots for 2 h in aerobic conditions”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Ca2+ nullified the effects of PLC inhibitors.

Treatment
Ca2+e0
Outcome
effect of PLC inhibitorse0
Direction
nullifiede0
Comparator
PLC inhibitorse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The presence of Ca2+ and A23187 (Ca2+ ionophore) nullified the effect of PLC inhibitors”

✓ located verbatim in the source · abstract, characters 255–342 · show in text ↑
t0

“Pre-treatment of rice roots for 2 h in aerobic conditions”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

A23187 nullified the effects of PLC inhibitors.

Treatment
A23187e0
Outcome
effect of PLC inhibitorse0
Direction
nullifiede0
Comparator
PLC inhibitorse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The presence of Ca2+ and A23187 (Ca2+ ionophore) nullified the effect of PLC inhibitors”

✓ located verbatim in the source · abstract, characters 255–342 · show in text ↑
t0

“Pre-treatment of rice roots for 2 h in aerobic conditions”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Neomycin abolished the anaerobic increase in inositol 1,4,5-triphosphate concentration.

Treatment
neomycine0
Gene
PLCe0
Outcome
concentration of the PLC product inositol 1,4,5-triphosphatee0
Direction
abolishede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Pre-treatment of rice roots with neomycin and C48/80 abolished the anaerobic increase in the concentration of the PLC product inositol 1,4,5-triphosphate”

✓ located verbatim in the source · abstract, characters 344–497 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

C48/80 abolished the anaerobic increase in inositol 1,4,5-triphosphate concentration.

Treatment
C48/80e0
Outcome
concentration of the PLC product inositol 1,4,5-triphosphatee0
Direction
abolishede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Pre-treatment of rice roots with neomycin and C48/80 abolished the anaerobic increase in the concentration of the PLC product inositol 1,4,5-triphosphate”

✓ located verbatim in the source · abstract, characters 344–497 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

PLC inhibitors attenuated aluminum fluoride stimulation of anaerobic signal transduction.

Treatment
PLC inhibitorse0
Outcome
Stimulation of the anaerobic signal transduction pathwaye0
Direction
attenuatede0
Comparator
aluminium fluoridee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Stimulation of the anaerobic signal transduction pathway with aluminium fluoride (G protein activator) was attenuated by PLC inhibitors”

✓ located verbatim in the source · abstract, characters 499–634 · show in text ↑
t0

“Pre-treatment of rice roots for 2 h in aerobic conditions”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The results are consistent with PLC participation in the anaerobic response.

Gene
PLCe0
Outcome
anaerobic responsee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“These findings are consistent with the participation of PLC in the anaerobic response”

✓ located verbatim in the source · abstract, characters 636–721 · show in text ↑
t0

“Pre-treatment of rice roots for 2 h in aerobic conditions”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑

neomycin · molecular: metabolites · Oryza sativa (rice)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.