Abstract
Sugar starvation-induced synthesis and extracellular liberation of α-amylase molecules in suspension-cultured cells of rice (Oryza sativa L.) required Ca2+, although the level of translatable α-amylase mRNA was not affected in the presence of Ca2+. Sugar depletion markedly stimulated Ca2+ uptake by rice cells and sucrose supplementation reduced it. Immunohistochemical and electron probe microanalyzer studies indicated an apparent resemblance between the distribution pattern of Ca2+ and that of α-amylase molecules induced in the sugar-depleted cells. Ca2+ uptake was reduced by sucrose, maltose, fructose, and glucose similarly at more than 5 mM, but was unaffected by mannitol (88 mM), 6-deoxy-D-glucose (10 mM), and 3-O-methyl-D-glucose (10 mM). Furthermore, an effective Ca2+ channel blocker, La3+ significantly inhibited the Ca2+ uptake and the synthesis and extracellular liberation of α-amylase molecules in the absence of sucrose, while a general P-type ATPase inhibitor, vanadate greatly stimulated both in the presence of sucrose. We concluded that, by controlling the Ca2+ uptake, metabolic sugars regulate the protein synthesis and posttranslational secretory processes of α-amylase molecules in rice cells.
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Claims 14
Claim · model-written summary
Sugar starvation-induced synthesis and extracellular liberation of alpha-amylase in rice cells required Ca2+.
- Taxon
- Rice
Oryza sativat0 - Treatment
- Sugar starvatione0
- Compound
- Ca2+e0
- Outcome
- synthesis and extracellular liberation of α-amylase moleculese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e0“Sugar starvation-induced synthesis and extracellular liberation of α-amylase molecules in suspension-cultured cells of rice (Oryza sativa L.) required Ca2+, although the level of translatable α-amylase mRNA was not affected in the presence of Ca2+”
Claim · model-written summary
The level of translatable alpha-amylase mRNA in rice cells was not affected by Ca2+ presence.
- Taxon
- RiceOryza sativat0
- Treatment
- presence of Ca2+e0
- Outcome
- level of translatable α-amylase mRNAe0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e0“Sugar starvation-induced synthesis and extracellular liberation of α-amylase molecules in suspension-cultured cells of rice (Oryza sativa L.) required Ca2+, although the level of translatable α-amylase mRNA was not affected in the presence of Ca2+”
Claim · model-written summary
Sugar depletion markedly stimulated Ca2+ uptake by rice cells.
- Taxon
- RiceOryza sativat0
- Treatment
- Sugar depletione1
- Outcome
- Ca2+ uptakee1
- Direction
- markedly stimulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e1“Sugar depletion markedly stimulated Ca2+ uptake by rice cells and sucrose supplementation reduced it”
Claim · model-written summary
Sucrose supplementation reduced Ca2+ uptake by rice cells.
- Taxon
- RiceOryza sativat0
- Treatment
- sucrose supplementatione1
- Outcome
- Ca2+ uptakee1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e1“Sugar depletion markedly stimulated Ca2+ uptake by rice cells and sucrose supplementation reduced it”
Claim · model-written summary
The distribution pattern of Ca2+ in rice cells apparently resembled that of alpha-amylase molecules induced in sugar-depleted cells.
- Taxon
- RiceOryza sativat0
- Treatment
- sugar-depleted cellse2
- Outcome
- distribution pattern of Ca2+e2
- Qualifier
- apparent resemblancee2
- Comparator
- α-amylase moleculese2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e2“Immunohistochemical and electron probe microanalyzer studies indicated an apparent resemblance between the distribution pattern of Ca2+ and that of α-amylase molecules induced in the sugar-depleted cells”
Claim · model-written summary
Maltose reduced Ca2+ uptake by rice cells at more than 5 mM.
- Taxon
- RiceOryza sativat0
- Treatment
- maltosee3
- Outcome
- Ca2+ uptakee3
- Direction
- reducede3
- Qualifier
- at more than 5 mMe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e3“Ca2+ uptake was reduced by sucrose, maltose, fructose, and glucose similarly at more than 5 mM, but was unaffected by mannitol (88 mM), 6-deoxy-D-glucose (10 mM), and 3-O-methyl-D-glucose (10 mM)”
Claim · model-written summary
Fructose reduced Ca2+ uptake by rice cells at more than 5 mM.
- Taxon
- RiceOryza sativat0
- Treatment
- fructosee3
- Outcome
- Ca2+ uptakee3
- Direction
- reducede3
- Qualifier
- at more than 5 mMe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e3“Ca2+ uptake was reduced by sucrose, maltose, fructose, and glucose similarly at more than 5 mM, but was unaffected by mannitol (88 mM), 6-deoxy-D-glucose (10 mM), and 3-O-methyl-D-glucose (10 mM)”
Claim · model-written summary
Glucose reduced Ca2+ uptake by rice cells at more than 5 mM.
- Taxon
- RiceOryza sativat0
- Treatment
- glucosee3
- Outcome
- Ca2+ uptakee3
- Direction
- reducede3
- Qualifier
- at more than 5 mMe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e3“Ca2+ uptake was reduced by sucrose, maltose, fructose, and glucose similarly at more than 5 mM, but was unaffected by mannitol (88 mM), 6-deoxy-D-glucose (10 mM), and 3-O-methyl-D-glucose (10 mM)”
Claim · model-written summary
Mannitol did not affect Ca2+ uptake by rice cells.
- Taxon
- RiceOryza sativat0
- Treatment
- mannitol (88 mM)e3
- Outcome
- Ca2+ uptakee3
- Direction
- unaffectede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e3“Ca2+ uptake was reduced by sucrose, maltose, fructose, and glucose similarly at more than 5 mM, but was unaffected by mannitol (88 mM), 6-deoxy-D-glucose (10 mM), and 3-O-methyl-D-glucose (10 mM)”
Claim · model-written summary
6-deoxy-D-glucose did not affect Ca2+ uptake by rice cells.
- Taxon
- RiceOryza sativat0
- Treatment
- 6-deoxy-D-glucose (10 mM)e3
- Outcome
- Ca2+ uptakee3
- Direction
- unaffectede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e3“Ca2+ uptake was reduced by sucrose, maltose, fructose, and glucose similarly at more than 5 mM, but was unaffected by mannitol (88 mM), 6-deoxy-D-glucose (10 mM), and 3-O-methyl-D-glucose (10 mM)”
Claim · model-written summary
3-O-methyl-D-glucose did not affect Ca2+ uptake by rice cells.
- Taxon
- RiceOryza sativat0
- Treatment
- 3-O-methyl-D-glucose (10 mM)e3
- Outcome
- Ca2+ uptakee3
- Direction
- unaffectede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e3“Ca2+ uptake was reduced by sucrose, maltose, fructose, and glucose similarly at more than 5 mM, but was unaffected by mannitol (88 mM), 6-deoxy-D-glucose (10 mM), and 3-O-methyl-D-glucose (10 mM)”
Claim · model-written summary
La3+, a Ca2+ channel blocker, significantly inhibited Ca2+ uptake and the synthesis and secretion of alpha-amylase in the absence of sucrose.
- Taxon
- RiceOryza sativat0
- Treatment
- La3+e4
- Outcome
- the Ca2+ uptake and the synthesis and extracellular liberation of α-amylase moleculese4
- Direction
- inhibitede4
- Qualifier
- in the absence of sucrosee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e4“La3+ significantly inhibited the Ca2+ uptake and the synthesis and extracellular liberation of α-amylase molecules in the absence of sucrose”
Claim · model-written summary
Vanadate greatly stimulated both Ca2+ uptake and alpha-amylase synthesis and secretion in the presence of sucrose.
- Taxon
- RiceOryza sativat0
- Treatment
- vanadatee5
- Outcome
- bothe5
- Direction
- greatly stimulatede5
- Qualifier
- in the presence of sucrosee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e5“vanadate greatly stimulated both in the presence of sucrose”
Claim · model-written summary
By controlling Ca2+ uptake, sugars regulate the protein synthesis and posttranslational secretory processes of alpha-amylase in rice cells.
- Taxon
- riceOryza sativae6
- Outcome
- protein synthesis and posttranslational secretory processes of α-amylase moleculese6
- Qualifier
- by controlling the Ca2+ uptakee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cultured Cells of Rice (Oryza sativa L.)”
e6“by controlling the Ca2+ uptake, metabolic sugars regulate the protein synthesis and posttranslational secretory processes of α-amylase molecules in rice cells”
The same questions in other papers
calcium(2+) · molecular: expression · Oryza sativa (rice)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.