Abstract
In the present investigation, an attempt has been made to understand the mechanism of light signal transduction in regulation of steady‐state transcript levels of the plastid genes psb A, psa A and rbc L in rice ( Oryza sativa L. ssp. indica cv. Pusa 169) seedlings. Red light irradiation of 5‐day‐old etiolated seedlings for 5 min upregulates the transcript levels of the above‐mentioned plastid genes, while far‐red light exposure subsequent to red light reverses this effect, indicating the involvement of phytochrome(s). Ca 2+ and the Ca 2+ ionophore, A23187 increase the level of transcripts in the dark‐grown seedlings. Ca 2+ chelators (EGTA and BAPTA) as well as Ca 2+ channel blockers (nifedipine and verapamil) suppress the accumulation of all transcripts in light. This suggests the involvement of Ca 2+ in the photoregulation of plastid genes. Okadaic acid, a specific inhibitor of protein phosphatase 1 and 2A (PP1 and PP2A), and sodium fluoride (NaF), a general inhibitor of protein phosphatases, significantly prevent the light‐induced increase in transcript levels of the plastid genes. Staurosporine, a protein kinase inhibitor, causes an increase in the transcript levels in darkness and a further enhancement in conjunction with light. The results suggest that light signal transduction downstream to phytochrome(s) involves Ca 2+ and phosphorylation for controlling the pattern of plastid gene expression. Phosphorylation inhibits light‐activated gene expression in chloroplasts.
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Claims 7
Claim · model-written summary
Red light raised plastid gene transcript levels in rice seedlings.
- Taxon
- rice
Oryza sativat0 - Treatment
- Red light irradiation of 5‐day‐old etiolated seedlings for 5 mine0
- Outcome
- transcript levelse0
- Direction
- upregulatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Red light irradiation of 5‐day‐old etiolated seedlings for 5 min upregulates the transcript levels of the above‐mentioned plastid genes, while far‐red light exposure subsequent to red light reverses this effect”
t0“rice (Oryza sativa)”
Claim · model-written summary
Far-red light after red light reversed transcript upregulation.
- Taxon
- riceOryza sativat0
- Treatment
- far‐red light exposure subsequent to red lighte0
- Outcome
- transcript levelse0
- Direction
- reversese0
- Comparator
- Red light irradiatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Red light irradiation of 5‐day‐old etiolated seedlings for 5 min upregulates the transcript levels of the above‐mentioned plastid genes, while far‐red light exposure subsequent to red light reverses this effect”
t0“rice (Oryza sativa)”
Claim · model-written summary
Ca 2+ and the Ca 2+ ionophore, A23187 altered plastid transcript accumulation.
- Taxon
- riceOryza sativat0
- Treatment
- Ca 2+ and the Ca 2+ ionophore, A23187e0
- Outcome
- level of transcriptse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ca 2+ and the Ca 2+ ionophore, A23187 increase the level of transcripts in the dark‐grown seedlings.”
t0“rice (Oryza sativa)”
Claim · model-written summary
Ca 2+ chelators (EGTA and BAPTA) as well as Ca 2+ channel blockers (nifedipine and verapamil) altered plastid transcript accumulation.
- Taxon
- riceOryza sativat0
- Treatment
- Ca 2+ chelators (EGTA and BAPTA) as well as Ca 2+ channel blockers (nifedipine and verapamil)e0
- Outcome
- accumulation of all transcriptse0
- Direction
- suppresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ca 2+ chelators (EGTA and BAPTA) as well as Ca 2+ channel blockers (nifedipine and verapamil) suppress the accumulation of all transcripts in light.”
t0“rice (Oryza sativa)”
Claim · model-written summary
Okadaic acid altered plastid transcript accumulation.
- Taxon
- riceOryza sativat0
- Treatment
- Okadaic acide0
- Outcome
- transcript levelse0
- Direction
- prevente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Okadaic acid, a specific inhibitor of protein phosphatase 1 and 2A (PP1 and PP2A), and sodium fluoride (NaF), a general inhibitor of protein phosphatases, significantly prevent the light‐induced increase in transcript levels of the plastid genes.”
t0“rice (Oryza sativa)”
Claim · model-written summary
sodium fluoride (NaF) altered plastid transcript accumulation.
- Taxon
- riceOryza sativat0
- Treatment
- sodium fluoride (NaF)e0
- Outcome
- transcript levelse0
- Direction
- prevente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Okadaic acid, a specific inhibitor of protein phosphatase 1 and 2A (PP1 and PP2A), and sodium fluoride (NaF), a general inhibitor of protein phosphatases, significantly prevent the light‐induced increase in transcript levels of the plastid genes.”
t0“rice (Oryza sativa)”
Claim · model-written summary
Staurosporine altered plastid transcript accumulation.
- Taxon
- riceOryza sativat0
- Treatment
- Staurosporinee0
- Outcome
- transcript levelse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Staurosporine, a protein kinase inhibitor, causes an increase in the transcript levels in darkness and a further enhancement in conjunction with light.”
t0“rice (Oryza sativa)”
The same questions in other papers
calcium(2+) · molecular: expression · Oryza sativa (rice)Positive and negative results
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- no effect
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staurosporine · molecular: expression · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
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okadaic acid · molecular: expression · Oryza sativa (rice)Positive and negative results
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radiation · molecular: expression · Oryza sativa (rice)Positive and negative results
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See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.