Abstract
Ionizing radiation has a substantial effect on physiological and biochemical processes in plants via induction of transcriptional changes and diverse genetic alterations. Previous microarray analysis showed that rice OsFBX322, which encodes a rice F-box protein, was downregulated in response to three types of ionizing radiation: gamma irradiation, ion beams, and cosmic rays. In order to characterize the radiation-responsive genes in rice, OsFBX322 was selected for further analysis. OsFBX322 expression patterns in response to radiation were confirmed using quantitative RT-PCR. Transient expression of a GFP-OsFBX322 fusion protein in tobacco leaf epidermis indicated that OsFBX322 is localized to the nucleus. To determine the effect of OsFBX322 expression on radiation response, OsFBX322 was overexpressed in Arabidopsis. Transgenic overexpression lines were more sensitive to gamma irradiation than control plants. These results suggest that OsFBX322 plays a negative role in the defense response to radiation in plants. In addition, we obtained four co-expression genes of OsFBX322 by specific co-expression networks using the ARANCE. Quantitative RT-PCR showed that the four genes were also downregulated after exposure to the three types of radiation. These results imply that the co-expressed genes may serve as key regulators in the radiation response pathway in plants.
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Claims 7
Claim · model-written summary
Rice OsFBX322 expression responses to radiation were confirmed by quantitative RT-PCR.
- Taxon
- rice
Oryza sativae1 - Treatment
- radiatione0
- Gene
- OsFBX322e0
- Outcome
- expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsFBX322 expression patterns in response to radiation were confirmed using quantitative RT-PCR”
e1“rice OsFBX322”
Claim · model-written summary
OsFBX322 protein localized to the nucleus in tobacco leaf epidermis.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- leaf epidermise0
- Gene
- OsFBX322e0
- Outcome
- localized to the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of a GFP-OsFBX322 fusion protein in tobacco leaf epidermis indicated that OsFBX322 is localized to the nucleus”
Claim · model-written summary
Arabidopsis lines overexpressing OsFBX322 were more gamma-sensitive than controls.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- gamma irradiatione0
- Gene
- OsFBX322e0
- Outcome
- sensitive to gamma irradiatione0
- Direction
- moree0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsFBX322 was overexpressed in Arabidopsis. Transgenic overexpression lines were more sensitive to gamma irradiation than control plants”
Claim · model-written summary
The authors infer that OsFBX322 negatively regulates defense against radiation.
- Taxon
- riceOryza sativat0
- Gene
- OsFBX322e0
- Outcome
- defense response to radiatione0
- Qualifier
- negative rolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsFBX322 plays a negative role in the defense response to radiation in plants”
t0“rice”
Claim · model-written summary
Four OsFBX322 co-expression genes were obtained from co-expression networks.
- Taxon
- riceOryza sativat0
- Gene
- OsFBX322e0
- Outcome
- co-expression genese0
- Value
- foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we obtained four co-expression genes of OsFBX322 by specific co-expression networks using the ARANCE”
t0“rice”
Claim · model-written summary
Four OsFBX322 co-expressed genes were downregulated after exposure to three radiation types.
- Taxon
- riceOryza sativat0
- Treatment
- three types of radiatione0
- Gene
- OsFBX322e1
- Outcome
- the four genese0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the four genes were also downregulated after exposure to the three types of radiation”
e1“four co-expression genes of OsFBX322”
t0“rice”
Claim · model-written summary
The authors suggest the co-expressed genes may regulate radiation response.
- Taxon
- riceOryza sativat0
- Gene
- co-expressed genese0
- Outcome
- radiation response pathwaye0
- Qualifier
- may serve as key regulatorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the co-expressed genes may serve as key regulators in the radiation response pathway in plants”
t0“rice”
The same questions in other papers
radiation · molecular: expression · Oryza sativa (rice)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- no effect
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.