Abstract
Okadaic acid (OKA), a potent and specific inhibitor of protein serine/threonine phosphatases 1 and 2A, induced the accumulation of NADH-glutamate synthase (GOGAT) mRNA within 4 h in rice (Oryza sativa L.) cell cultures. In contrast to the transient accumulation of NADH-GOGAT mRNA by NH(4)(+), OKA caused a continuous accumulation for at least 24 h. The induction of NADH-GOGAT mRNA by OKA was not inhibited in the presence of methionine sulfoximine, which inhibited the NH(4)(+)-induced accumulation of mRNA. These results suggest that the OKA-sensitive protein phosphatase is involved in the regulation of NADH-GOGAT gene expression and probably plays a role in the signal transduction pathway downstream from NH(4)(+), although a signal transduction pathway other than that of nitrogen sensing could be responsible. Nuclear run-on assays demonstrated that the accumulation of NADH-GOGAT mRNA induced by the supply of either NH(4)(+) or OKA was mainly regulated at the transcription level. OKA effects were synergistic to the NH(4)(+)-induced expression of the NADH-GOGAT gene. In the presence of K-252a, a protein kinase inhibitor, the accumulation of NADH-GOGAT mRNA induced by either NH(4)(+) or OKA was reduced. The possible roles of protein phosphatases in the regulation of NADH-GOGAT gene expression are discussed.
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Claims 8
Claim · model-written summary
Okadaic acid induced accumulation of NADH-GOGAT mRNA within 4 h in rice cell cultures.
- Taxon
- Rice
Oryza sativat0 - Treatment
- Okadaic acid (OKA)e0
- Gene
- NADH-glutamate synthase (GOGAT)e0
- Outcome
- accumulation of NADH-glutamate synthase (GOGAT) mRNAe0
- Direction
- inducede0
- Value
- within 4 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Okadaic acid (OKA), a potent and specific inhibitor of protein serine/threonine phosphatases 1 and 2A, induced the accumulation of NADH-glutamate synthase (GOGAT) mRNA within 4 h in rice (Oryza sativa L.) cell cultures”
t0“Rice Cell Cultures”
Claim · model-written summary
OKA caused a continuous accumulation of NADH-GOGAT mRNA for at least 24 h, in contrast to the transient accumulation by NH4+.
- Taxon
- RiceOryza sativat0
- Treatment
- OKAe0
- Gene
- NADH-GOGATe0
- Outcome
- accumulation of NADH-GOGAT mRNAe0
- Direction
- continuous accumulatione0
- Value
- at least 24 he0
- Comparator
- NH(4)(+)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In contrast to the transient accumulation of NADH-GOGAT mRNA by NH(4)(+), OKA caused a continuous accumulation for at least 24 h”
t0“Rice Cell Cultures”
Claim · model-written summary
Methionine sulfoximine did not inhibit the OKA-induced accumulation of NADH-GOGAT mRNA.
- Taxon
- RiceOryza sativat0
- Treatment
- methionine sulfoximinee0
- Gene
- NADH-GOGATe0
- Outcome
- induction of NADH-GOGAT mRNA by OKAe0
- Direction
- not inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of NADH-GOGAT mRNA by OKA was not inhibited in the presence of methionine sulfoximine, which inhibited the NH(4)(+)-induced accumulation of mRNA”
t0“Rice Cell Cultures”
Claim · model-written summary
Methionine sulfoximine inhibited the NH4+-induced accumulation of NADH-GOGAT mRNA.
- Taxon
- RiceOryza sativat0
- Treatment
- methionine sulfoximinee0
- Outcome
- NH(4)(+)-induced accumulation of mRNAe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of NADH-GOGAT mRNA by OKA was not inhibited in the presence of methionine sulfoximine, which inhibited the NH(4)(+)-induced accumulation of mRNA”
t0“Rice Cell Cultures”
Claim · model-written summary
The authors infer that an OKA-sensitive protein phosphatase is involved in regulating NADH-GOGAT gene expression.
- Taxon
- RiceOryza sativat0
- Gene
- OKA-sensitive protein phosphatasee0
- Outcome
- NADH-GOGAT gene expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the OKA-sensitive protein phosphatase is involved in the regulation of NADH-GOGAT gene expression”
t0“Rice Cell Cultures”
Claim · model-written summary
Nuclear run-on assays showed that NADH-GOGAT mRNA accumulation induced by NH4+ or OKA was mainly regulated at the transcription level.
- Taxon
- RiceOryza sativat0
- Treatment
- NH(4)(+) or OKAe0
- Gene
- NADH-GOGATe0
- Outcome
- accumulation of NADH-GOGAT mRNAe0
- Qualifier
- mainly regulated at the transcription levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nuclear run-on assays demonstrated that the accumulation of NADH-GOGAT mRNA induced by the supply of either NH(4)(+) or OKA was mainly regulated at the transcription level”
t0“Rice Cell Cultures”
Claim · model-written summary
OKA effects were synergistic to NH4+-induced expression of the NADH-GOGAT gene.
- Taxon
- RiceOryza sativat0
- Treatment
- OKAe0
- Gene
- NADH-GOGATe0
- Outcome
- NH(4)(+)-induced expression of the NADH-GOGAT genee0
- Direction
- synergistice0
- Comparator
- NH(4)(+)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OKA effects were synergistic to the NH(4)(+)-induced expression of the NADH-GOGAT gene”
t0“Rice Cell Cultures”
Claim · model-written summary
K-252a, a protein kinase inhibitor, reduced NADH-GOGAT mRNA accumulation induced by NH4+ or OKA.
- Taxon
- RiceOryza sativat0
- Treatment
- K-252ae0
- Gene
- NADH-GOGATe0
- Outcome
- accumulation of NADH-GOGAT mRNAe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the presence of K-252a, a protein kinase inhibitor, the accumulation of NADH-GOGAT mRNA induced by either NH(4)(+) or OKA was reduced”
t0“Rice Cell Cultures”
The same questions in other papers
okadaic acid · molecular: expression · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.