Abstract
Two cDNA clones, pOS-ACO2 and pOS-ACO3, encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase were isolated from rice seedling cDNA library. pOS-ACO3 is a 1,299 bp full-length clone encoding 321 amino acids (Mr=35.9 kDa), while pOS-ACO2 is 1,072 bp long and is a partial cDNA clone encoding 314 amino acids. These two deduced amino acid sequences share 70% identity, and display a high degree of sequence identity (72-92%) with previously isolated pOS-ACO1 of deepwater rice. The chromosomal location studies show that OS-ACO2 is positioned on the long arm of chromosome 9, while OS-ACO3 on the long arm of chromosome 2 of rice genome. A marked increase in the level of OS-ACO2 transcript was observed in IAA-treated etiolated rice seedlings, whereas the OS-ACO3 mRNA was greatly accumulated by ethylene treatment. Results of ethylene inhibitor studies indicated that auxin promotion of the OS-ACO2 transcription was not mediated through the action of auxin-induced ethylene. Thus, it appears that there are two groups of ACC oxidase transcripts in rice plants, either auxin-induced or ethylene-induced. The auxin-induced OS-ACO2 expression was partially inhibited by ethylene, while ethylene induction of OS-ACO3 transcription was completely blocked by auxin. These results indicate that the expression of ACC oxidase genes is regulated by complex hormonal networks in a gene specific manner in rice seedlings. Okadaic acid, a potent inhibitor of protein phosphatase, effectively suppressed the IAA induction of OS-ACO2 expression, suggesting that protein dephosphorylation plays a role in the induction of ACC oxidase by auxin. A scheme of the multiple regulatory pathways for the expression of ACC oxidase gene family by auxin, ethylene and protein phosphatase is presented.
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Claims 14
Claim · model-written summary
Rice pOS-ACO2 and pOS-ACO3 cDNA clones encoding ACC oxidase were isolated.
- Taxon
- rice
Oryza sativae0 - Gene
- pOS-ACO2 and pOS-ACO3e0
- Outcome
- were isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two cDNA clones, pOS-ACO2 and pOS-ACO3, encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase were isolated from rice seedling cDNA library.”
Claim · model-written summary
pOS-ACO3 is a 1,299 bp full length clone encoding 321 amino acids.
- Taxon
- riceOryza sativae1
- Gene
- pOS-ACO3e0
- Outcome
- full-length clonee0
- Value
- 1,299 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pOS-ACO3 is a 1,299 bp full-length clone encoding 321 amino acids (Mr=35.9 kDa)”
e1“rice seedling cDNA library”
Claim · model-written summary
pOS-ACO2 is a 1,072 bp partial cDNA clone encoding 314 amino acids.
- Taxon
- riceOryza sativae1
- Gene
- pOS-ACO2e0
- Outcome
- partial cDNA clonee0
- Value
- 1,072 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pOS-ACO2 is 1,072 bp long and is a partial cDNA clone encoding 314 amino acids”
e1“rice seedling cDNA library”
Claim · model-written summary
The two rice ACC oxidase sequences share 70% identity.
- Taxon
- riceOryza sativae1
- Outcome
- identitye0
- Value
- 70%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These two deduced amino acid sequences share 70% identity”
e1“rice seedling cDNA library”
Claim · model-written summary
The sequences share 72-92% identity with pOS-ACO1 of deepwater rice.
- Taxon
- riceOryza sativae0
- Gene
- pOS-ACO1e0
- Outcome
- sequence identitye0
- Value
- 72-92%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“display a high degree of sequence identity (72-92%) with previously isolated pOS-ACO1 of deepwater rice”
Claim · model-written summary
OS-ACO2 maps to the long arm of rice chromosome 9.
- Taxon
- riceOryza sativae1
- Gene
- OS-ACO2e0
- Outcome
- long arm of chromosome 9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OS-ACO2 is positioned on the long arm of chromosome 9”
e1“rice genome”
Claim · model-written summary
OS-ACO3 maps to the long arm of rice chromosome 2.
- Taxon
- riceOryza sativae0
- Gene
- OS-ACO3e0
- Outcome
- long arm of chromosome 2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OS-ACO3 on the long arm of chromosome 2 of rice genome”
Claim · model-written summary
IAA increased OS-ACO2 transcript in etiolated rice seedlings.
- Taxon
- riceOryza sativae0
- Organ / stage
- etiolated rice seedlingse0
- Treatment
- IAA-treatede0
- Gene
- OS-ACO2e0
- Outcome
- level of OS-ACO2 transcripte0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A marked increase in the level of OS-ACO2 transcript was observed in IAA-treated etiolated rice seedlings”
Claim · model-written summary
Ethylene greatly increased OS-ACO3 mRNA accumulation.
- Taxon
- riceOryza sativae1
- Treatment
- ethylene treatmente0
- Gene
- OS-ACO3e0
- Outcome
- OS-ACO3 mRNAe0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the OS-ACO3 mRNA was greatly accumulated by ethylene treatment”
e1“gene specific manner in rice seedlings.”
Claim · model-written summary
Auxin promotion of OS-ACO2 transcription was not mediated through auxin-induced ethylene.
- Taxon
- riceOryza sativae1
- Gene
- OS-ACO2e0
- Outcome
- OS-ACO2 transcriptione0
- Qualifier
- not mediated through the action of auxin-induced ethylenee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“auxin promotion of the OS-ACO2 transcription was not mediated through the action of auxin-induced ethylene”
e1“gene specific manner in rice seedlings.”
Claim · model-written summary
Ethylene partially inhibited auxin-induced OS-ACO2 expression.
- Taxon
- riceOryza sativae1
- Treatment
- ethylenee0
- Gene
- OS-ACO2e0
- Outcome
- auxin-induced OS-ACO2 expressione0
- Direction
- inhibitede0
- Qualifier
- partiallye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The auxin-induced OS-ACO2 expression was partially inhibited by ethylene”
e1“gene specific manner in rice seedlings.”
Claim · model-written summary
Auxin completely blocked ethylene-induced OS-ACO3 transcription.
- Taxon
- riceOryza sativae1
- Treatment
- auxine0
- Gene
- OS-ACO3e0
- Outcome
- ethylene induction of OS-ACO3 transcriptione0
- Direction
- blockede0
- Qualifier
- completelye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene induction of OS-ACO3 transcription was completely blocked by auxin”
e1“gene specific manner in rice seedlings.”
Claim · model-written summary
Okadaic acid suppressed IAA induction of OS-ACO2 expression.
- Taxon
- riceOryza sativae1
- Treatment
- Okadaic acide0
- Gene
- OS-ACO2e0
- Outcome
- IAA induction of OS-ACO2 expressione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Okadaic acid, a potent inhibitor of protein phosphatase, effectively suppressed the IAA induction of OS-ACO2 expression”
e1“gene specific manner in rice seedlings.”
Claim · model-written summary
The authors infer that protein dephosphorylation participates in auxin induction of ACC oxidase.
- Taxon
- riceOryza sativae1
- Gene
- ACC oxidasee0
- Outcome
- induction of ACC oxidasee0
- Qualifier
- protein dephosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protein dephosphorylation plays a role in the induction of ACC oxidase by auxin”
e1“gene specific manner in rice seedlings.”
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