Abstract
Auxin is well known to be a key regulator that acts in almost all physiological processes during plant growth, and in interactions between plants and microbes. However, to date, the regulatory mechanisms underlying auxin-mediated plant-arbuscular mycorrhizal (AM) fungi symbiosis have not been well deciphered. Previously we identified a GH3 gene, SlGH3.4, strongly responsive to both auxin induction and mycorrhizal symbiosis. Here, we reported a refined dissection of the SlGH3.4 promoter activity using the β-glucuronidase (GUS) reporter. The SlGH3.4 promoter could drive GUS expression strongly in mycorrhizal roots of soybean and rice plants, and in IAA-treated soybean roots, but not in IAA-treated rice roots. A promoter deletion assay revealed three cis-acting motifs, i.e. the auxin-responsive element, AuxRE, and two newly identified motifs named MYCRS1 and MYCRS2, involved in the activation of auxin- and AM-mediated expression of SlGH3.4. Deletion of the AuxRE from the SlGH3.4 promoter caused almost complete abolition of GUS staining in response to external IAA induction. Seven repeats of AuxRE fused to the Cauliflower mosaic virus (CaMV) 35S minimal promoter could direct GUS expression in both IAA-treated and AM fungal-colonized roots of tobacco plants. Four repeats of MYCRS1 or MYCRS2 fused to the CaMV35S minimal promoter was sufficient to drive GUS expression in arbuscule-containing cells, but not in IAA-treated tobacco roots. In summary, our results offer new insights into the molecular mechanisms underlying the potential cross-talk between the auxin and the AM regulatory pathways in modulating the expression of AM-responsive GH3 genes in diverse mycorrhizal plants.
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Claims 12
Claim · model-written summary
The SlGH3.4 promoter drove strong GUS expression in mycorrhizal soybean roots.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- rootse0
- Gene
- SlGH3.4e0
- Outcome
- GUS expressione0
- Direction
- stronglye0
Not reportedInteractor
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato GH3 Gene”
e0“The SlGH3.4 promoter could drive GUS expression strongly in mycorrhizal roots of soybean and rice plants”
Claim · model-written summary
The SlGH3.4 promoter drove strong GUS expression in mycorrhizal rice roots.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The SlGH3.4 promoter could drive GUS expression strongly in mycorrhizal roots of soybean and rice plants”
Claim · model-written summary
The SlGH3.4 promoter drove GUS expression in IAA treated soybean roots.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- rootse0
- Treatment
- IAA-treatede0
- Outcome
- IAA-treated soybean rootse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in IAA-treated soybean roots, but not in IAA-treated rice roots.”
Claim · model-written summary
The SlGH3.4 promoter did not drive GUS expression in IAA treated rice roots.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- rootse0
- Treatment
- IAA-treatede0
- Outcome
- GUS expressione0
- Direction
- but note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The SlGH3.4 promoter could drive GUS expression strongly in mycorrhizal roots of soybean and rice plants, and in IAA-treated soybean roots, but not in IAA-treated rice roots.”
Claim · model-written summary
AuxRE, MYCRS1 and MYCRS2 were implicated in auxin and AM mediated SlGH3.4 activation.
- Taxon
- Tomato
Solanum lycopersicumt0 - Gene
- SlGH3.4e0
- Outcome
- activation of auxin- and AM-mediated expression of SlGH3.4e0
- Qualifier
- AuxRE, and two newly identified motifs named MYCRS1 and MYCRS2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato GH3 Gene”
e0“three cis-acting motifs, i.e. the auxin-responsive element, AuxRE, and two newly identified motifs named MYCRS1 and MYCRS2, involved in the activation of auxin- and AM-mediated expression of SlGH3.4.”
Claim · model-written summary
Deleting AuxRE almost abolished IAA induced GUS staining.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Deletion of the AuxREe0
- Gene
- SlGH3.4e0
- Outcome
- GUS staininge0
- Direction
- almost complete abolitione0
- Qualifier
- external IAA inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato GH3 Gene”
e0“Deletion of the AuxRE from the SlGH3.4 promoter caused almost complete abolition of GUS staining in response to external IAA induction.”
Claim · model-written summary
Seven AuxRE repeats drove GUS expression in IAA treated tobacco roots.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- rootse0
- Treatment
- Seven repeats of AuxREe0
- Outcome
- GUS expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seven repeats of AuxRE fused to the Cauliflower mosaic virus (CaMV) 35S minimal promoter could direct GUS expression in both IAA-treated and AM fungal-colonized roots of tobacco plants.”
Claim · model-written summary
Seven AuxRE repeats drove GUS expression in AM fungal colonized tobacco roots.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- rootse0
- Treatment
- Seven repeats of AuxREe0
- Interactor
- AM fungale0symbiont
- Outcome
- GUS expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seven repeats of AuxRE fused to the Cauliflower mosaic virus (CaMV) 35S minimal promoter could direct GUS expression in both IAA-treated and AM fungal-colonized roots of tobacco plants.”
Claim · model-written summary
Four MYCRS1 repeats drove GUS expression in arbuscule containing cells.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- arbuscule-containing cellse0
- Treatment
- Four repeats of MYCRS1e0
- Outcome
- GUS expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four repeats of MYCRS1 or MYCRS2 fused to the CaMV35S minimal promoter was sufficient to drive GUS expression in arbuscule-containing cells, but not in IAA-treated tobacco roots.”
Claim · model-written summary
Four MYCRS2 repeats drove GUS expression in arbuscule containing cells.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- arbuscule-containing cellse0
- Treatment
- MYCRS2e0
- Outcome
- GUS expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four repeats of MYCRS1 or MYCRS2 fused to the CaMV35S minimal promoter was sufficient to drive GUS expression in arbuscule-containing cells, but not in IAA-treated tobacco roots.”
Claim · model-written summary
Four MYCRS1 repeats did not drive GUS expression in IAA treated tobacco roots.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- rootse0
- Treatment
- Four repeats of MYCRS1e0
- Outcome
- GUS expressione0
- Direction
- but note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four repeats of MYCRS1 or MYCRS2 fused to the CaMV35S minimal promoter was sufficient to drive GUS expression in arbuscule-containing cells, but not in IAA-treated tobacco roots.”
Claim · model-written summary
Four MYCRS2 repeats did not drive GUS expression in IAA treated tobacco roots.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- rootse0
- Treatment
- MYCRS2e0
- Outcome
- GUS expressione0
- Direction
- but note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four repeats of MYCRS1 or MYCRS2 fused to the CaMV35S minimal promoter was sufficient to drive GUS expression in arbuscule-containing cells, but not in IAA-treated tobacco roots.”
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