Abstract
Calcineurin B-like protein Interacting (CIPK) protein kinase plays an important role in stress signal transduction in plant. One full-length cDNA encoding a CIPKs homologue was isolated from maize (Zea mays) by RT-PCR and named as ZmCIPK1 (GenBank accession No.EF158034). The predicted ZmCIPK1 protein had 465 amino acids with an estimated molecular mass of 51.98 kD and an isoelectric point of 6.92. The putative kinase catalytic domain of ZmCIPK1 contained 11 subdomains that were typical in protein kinase. The conserved 24-amino acid motif within C-terminal non-kinase region of the CIPKs was also found in ZmCIPK1. RT-PCR analysis indicated that ZmCIPK1 expression was up-regulated obviously by mannitol, salt, ABA and low-temperature (4 ℃) in young leaves of maize and kept high level during 24 h. These results demonstrate that ZmCIPK1 is a novel CIPK gene and responds to multiple abiotic signals in maize.
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Claims 10
Claim · model-written summary
A full-length maize CIPK homologue, ZmCIPK1, was isolated.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One full-length cDNA encoding a CIPKs homologue was isolated from maize (Zea mays) by RT-PCR and named as ZmCIPK1”
Claim · model-written summary
Predicted ZmCIPK1 has 465 amino acids.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The predicted ZmCIPK1 protein had 465 amino acids with an estimated molecular mass of 51.98 kD and an isoelectric point of 6.92”
e1“maize (Zea mays) by RT-PCR”
Claim · model-written summary
Predicted ZmCIPK1 has a mass of 51.98 kD.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The predicted ZmCIPK1 protein had 465 amino acids with an estimated molecular mass of 51.98 kD and an isoelectric point of 6.92”
e1“maize (Zea mays) by RT-PCR”
Claim · model-written summary
Predicted ZmCIPK1 has an isoelectric point of 6.92.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The predicted ZmCIPK1 protein had 465 amino acids with an estimated molecular mass of 51.98 kD and an isoelectric point of 6.92”
e1“maize (Zea mays) by RT-PCR”
Claim · model-written summary
ZmCIPK1 contains 11 typical kinase catalytic subdomains.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The putative kinase catalytic domain of ZmCIPK1 contained 11 subdomains that were typical in protein kinase”
e1“maize (Zea mays) by RT-PCR”
Claim · model-written summary
ZmCIPK1 contains the conserved CIPK C-terminal 24-amino-acid motif.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The conserved 24-amino acid motif within C-terminal non-kinase region of the CIPKs was also found in ZmCIPK1”
e1“maize (Zea mays) by RT-PCR”
Claim · model-written summary
Mannitol up-regulated ZmCIPK1 expression in young maize leaves.
- Organ / stage
- young leavese0
- Treatment
- mannitole0
- Gene
- ZmCIPK1e0
- Outcome
- expressione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmCIPK1 expression was up-regulated obviously by mannitol, salt, ABA and low-temperature (4 ℃) in young leaves of maize and kept high level during 24 h”
Claim · model-written summary
Salt up-regulated ZmCIPK1 expression in young maize leaves.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmCIPK1 expression was up-regulated obviously by mannitol, salt, ABA and low-temperature (4 ℃) in young leaves of maize and kept high level during 24 h”
Claim · model-written summary
ABA up-regulated ZmCIPK1 expression in young maize leaves.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmCIPK1 expression was up-regulated obviously by mannitol, salt, ABA and low-temperature (4 ℃) in young leaves of maize and kept high level during 24 h”
Claim · model-written summary
Low temperature up-regulated ZmCIPK1 expression in young maize leaves.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmCIPK1 expression was up-regulated obviously by mannitol, salt, ABA and low-temperature (4 ℃) in young leaves of maize and kept high level during 24 h”
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See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.