Abstract
Aldehyde dehydrogenases(ALDHs) represent a large protein family, which includes several members that catalyze the oxidation of an aldehyde to its corresponding carboxylic acid in plants. Genes encoding members of the ALDH7 subfamily have been suggested to play important roles in various stress adaptations in plants. In this study, quantitative RT-PCR analysis revealed that a maize ALDH7 subfamily member(ZmALDH7B6) was constitutively expressed in various organs, including roots, leaves, immature ears, tassels, and developing seeds. The abundance of ZmALDH7B6 mRNA transcripts in maize roots was increased by ammonium, NaCl, and mannitol treatments. To further analyze tissue-specific and stress-induced expression patterns, the 1.5-kb 5′-flanking ZmALDH7B6 promoter region was fused to the β-glucuronidase(GUS) reporter gene and introduced into maize plants. In roots of independent transgenic lines, there was significant induction of GUS activity in response to ammonium supply, confirming ammonium-dependent expression of ZmALDH7B6 at the transcript level. Histochemical staining showed that GUS activity driven by the ZmALDH7B6 promoter was mainly localized in the vascular tissues of maize roots. These results suggested that ZmALDH7B6 is induced by multiple environmental stresses in maize roots, and may play a role in detoxifying aldehydes, particularly in vascular tissue.
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Claims 7
Claim · model-written summary
ZmALDH7B6 was constitutively expressed in roots, leaves, immature ears, tassels and developing seeds of maize.
- Organ / stage
- roots, leaves, immature ears, tassels, and developing seedse1
- Gene
- ZmALDH7B6e1
- Outcome
- constitutively expressede1
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a maize ALDH7 subfamily member(ZmALDH7B6) was constitutively expressed in various organs, including roots, leaves, immature ears, tassels, and developing seeds”
Claim · model-written summary
ZmALDH7B6 mRNA abundance in maize roots was increased by ammonium.
- Organ / stage
- rootse1
- Treatment
- ammoniume1
- Gene
- ZmALDH7B6e1
- Outcome
- abundance of ZmALDH7B6 mRNA transcriptse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The abundance of ZmALDH7B6 mRNA transcripts in maize roots was increased by ammonium, NaCl, and mannitol treatments”
Claim · model-written summary
ZmALDH7B6 mRNA abundance in maize roots was increased by NaCl.
- Organ / stage
- rootse1
- Treatment
- NaCle1
- Gene
- ZmALDH7B6e1
- Outcome
- abundance of ZmALDH7B6 mRNA transcriptse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The abundance of ZmALDH7B6 mRNA transcripts in maize roots was increased by ammonium, NaCl, and mannitol treatments”
Claim · model-written summary
ZmALDH7B6 mRNA abundance in maize roots was increased by mannitol.
- Organ / stage
- rootse1
- Treatment
- mannitole1
- Gene
- ZmALDH7B6e1
- Outcome
- abundance of ZmALDH7B6 mRNA transcriptse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The abundance of ZmALDH7B6 mRNA transcripts in maize roots was increased by ammonium, NaCl, and mannitol treatments”
Claim · model-written summary
In roots of independent transgenic maize lines carrying the ZmALDH7B6 promoter fused to GUS, GUS activity was significantly induced by ammonium supply.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In roots of independent transgenic lines, there was significant induction of GUS activity in response to ammonium supply”
e2“introduced into maize plants”
Claim · model-written summary
GUS activity driven by the ZmALDH7B6 promoter was mainly localized in the vascular tissues of maize roots.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“GUS activity driven by the ZmALDH7B6 promoter was mainly localized in the vascular tissues of maize roots”
Claim · model-written summary
The authors suggest that ZmALDH7B6 may play a role in detoxifying aldehydes, particularly in vascular tissue of maize roots.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“may play a role in detoxifying aldehydes, particularly in vascular tissue”
e2“ZmALDH7B6 is induced by multiple environmental stresses in maize roots”
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