Growth and Cytology of Celery Plants Derived from Tissue Cultures

Abstract

Callus was initiated from petiole explants of 3-month-old celery plants and sub-cultured at monthly intervals on a modified Murashige and Skoog medium. 6 months later a further batch of callus was initiated, again from 3-month-old plants. When the first batch of callus had been sub-cultured for 15 months, and the second batch for 9 months, the callus from both sources was placed on a hormone-free medium to allow plants to form. Normal seedlings were grown at the same time and when the plantlets were large enough both the plantlets and the seedlings were transferred to individual pots and grown to maturity in a greenhouse. The petiole height, width and number of petioles were measured at intervals during the growing period and at one stage root tips were excised from a number of plants in each group and the chromosome number counted. The growth and cytological data showed that the plants derived from tissue culture were not affected by difference in sub-culture time and were very similar to the seedling plants.

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