Abstract
The pollen keep viability is one of the most important conditions for walnut cross breeding. However, the blossom time of different varieties is different and the male flower is earlier than the most female. As the flowering time is different, it needs to save the male pollen. The purpose of this test is to know the viability and the method to keep viability of different walnut varieties. The pollen from Xilin 3, Xiluo 3 and Xiangling were used as materials, they were placed under room temperature or the 4 ℃ refrigerator and the MTT staining was used to determination the changes of vitality. The results show that: the pollen viability is different between different varieties. It is better to keep the viability in refrigerator than under room temperature, the viability could be kept for about 10 days in the refrigerator but only 6 days under room temperature.
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Claims 4
Claim · model-written summary
Pollen viability differed among walnut varieties.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the pollen viability is different between different varieties”
t0“Walnuts”
Claim · model-written summary
Refrigeration preserved pollen viability better than room temperature.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“It is better to keep the viability in refrigerator than under room temperature”
t0“Walnuts”
Claim · model-written summary
Pollen viability persisted about 10 days under refrigeration.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the viability could be kept for about 10 days in the refrigerator”
t0“Walnuts”
Claim · model-written summary
Pollen viability persisted only 6 days at room temperature.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“only 6 days under room temperature”
e1“the viability could be kept”
t0“Walnuts”