Abstract
To establish an asexual propagation system of mulberry hairy root,the effect of different concentration of plant growth regulators 6-BA and NAA on the mulberry hairy root growth in MS medium was studied.Also,PCR examination was carried out.The results showed that the best medium for callus induction was MS+0.2 mg/L 6-BA+2.0 mg/L NAA,and the best medium for adventitious buds induction was MS+1.0 mg/L 6-BA+0.1 mg/L NAA,with the ratio of adventitious buds induction of 16.7% within 3 weeks.The roots of plantlets from the adventitious buds were exuberant.PCR examination showed that rolB gene was successfully amplified from the leaf genome.
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Claims 4
Claim · model-written summary
MS plus 0.2 mg/L 6-BA and 2.0 mg/L NAA was the best medium for callus induction.
Not reportedComparator
Verbatim
e0“the best medium for callus induction was MS+0.2 mg/L 6-BA+2.0 mg/L NAA”
t0“Mulberry Hairy Root”
Claim · model-written summary
MS plus 1.0 mg/L 6-BA and 0.1 mg/L NAA was best for bud induction, with 16.7% induction within three weeks.
- Taxon
- Mulberryt0
- Treatment
- MS+1.0 mg/L 6-BA+0.1 mg/L NAAe0
- Outcome
- adventitious buds inductione0
- Direction
- beste0
- Value
- 16.7%e0
- Qualifier
- within 3 weekse0
Not reportedComparator
Verbatim
e0“the best medium for adventitious buds induction was MS+1.0 mg/L 6-BA+0.1 mg/L NAA,with the ratio of adventitious buds induction of 16.7% within 3 weeks”
t0“Mulberry Hairy Root”
Claim · model-written summary
Plantlets from adventitious buds had exuberant roots.
Verbatim
e0“The roots of plantlets from the adventitious buds were exuberant”
t0“Mulberry Hairy Root”
Claim · model-written summary
PCR amplified rolB from the leaf genome.
Verbatim
e0“PCR examination showed that rolB gene was successfully amplified from the leaf genome”
t0“Mulberry Hairy Root”
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