Abstract
Glutamate decarboxylase(GAD)was purified by using(NH4)2SO4 fractionation and DEAE-Sephrose FF ion exchange chromatography. GAD was purified 8.8-folds. The specific activity was 30.1 U/mg,and the enzymatic activity recovery was 45.5%. The optimum temperature of GAD was 40℃,and it was inactive at 70℃. The optimum pH was 5.5,and it was stable at pH5.5~9.0. Km values for GAD and PLP were determined at 28.45 and 1.13μmol/L,respectively. Chemicals reagent s such as KI,Ag+,SDS and acetic acid decreased the enzyme activity,but Ca2+ increased the GAD activity greatly.
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Claims 14
Claim · model-written summary
Rice bran GAD was purified 8.8-folds.
- Taxon
- rice
Oryza sativat0 - Gene
- GADe0
- Outcome
- purifiede0
- Value
- 8.8-foldse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GAD was purified 8.8-folds.”
t0“rice bran”
Claim · model-written summary
The specific activity of rice bran GAD was 30.1 U/mg.
- Taxon
- riceOryza sativat0
- Outcome
- specific activitye0
- Value
- 30.1 U/mge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The specific activity was 30.1 U/mg”
t0“rice bran”
Claim · model-written summary
Enzymatic activity recovery was 45.5%.
- Taxon
- riceOryza sativat0
- Outcome
- enzymatic activity recoverye0
- Value
- 45.5%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the enzymatic activity recovery was 45.5%.”
t0“rice bran”
Claim · model-written summary
GAD had an optimum temperature of 40℃.
- Taxon
- riceOryza sativat0
- Gene
- GADe0
- Outcome
- optimum temperaturee0
- Value
- 40℃e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimum temperature of GAD was 40℃”
t0“rice bran”
Claim · model-written summary
GAD was inactive at 70℃.
- Taxon
- riceOryza sativat0
- Treatment
- 70℃e0
- Gene
- GADe0
- Outcome
- inactivee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimum temperature of GAD was 40℃,and it was inactive at 70℃.”
t0“rice bran”
Claim · model-written summary
The optimum pH of rice bran GAD was 5.5.
- Taxon
- riceOryza sativat0
- Outcome
- optimum pHe0
- Value
- 5.5e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimum pH was 5.5”
t0“rice bran”
Claim · model-written summary
Rice bran GAD was stable at pH5.5~9.0.
- Taxon
- riceOryza sativat0
- Outcome
- stablee0
- Qualifier
- pH5.5~9.0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was stable at pH5.5~9.0.”
t0“rice bran”
Claim · model-written summary
The Km value for GAD was 28.45 μmol/L, with the unit shared in the reported pair.
- Taxon
- riceOryza sativat0
- Gene
- GADe0
- Outcome
- Km valuese0
- Value
- 28.45e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Km values for GAD and PLP were determined at 28.45 and 1.13μmol/L,respectively.”
t0“rice bran”
Claim · model-written summary
The Km value for PLP was 1.13μmol/L.
- Taxon
- riceOryza sativat0
- Compound
- PLPe0
- Outcome
- Km valuese0
- Value
- 1.13μmol/Le0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Km values for GAD and PLP were determined at 28.45 and 1.13μmol/L,respectively.”
t0“rice bran”
Claim · model-written summary
KI decreased rice bran enzyme activity.
- Taxon
- riceOryza sativat0
- Treatment
- KIe0
- Outcome
- enzyme activitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chemicals reagent s such as KI,Ag+,SDS and acetic acid decreased the enzyme activity”
t0“rice bran”
Claim · model-written summary
Ag+ decreased rice bran enzyme activity.
- Taxon
- riceOryza sativat0
- Treatment
- Ag+e0
- Outcome
- enzyme activitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chemicals reagent s such as KI,Ag+,SDS and acetic acid decreased the enzyme activity”
t0“rice bran”
Claim · model-written summary
SDS decreased rice bran enzyme activity.
- Taxon
- riceOryza sativat0
- Treatment
- SDSe0
- Outcome
- enzyme activitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chemicals reagent s such as KI,Ag+,SDS and acetic acid decreased the enzyme activity”
t0“rice bran”
Claim · model-written summary
Acetic acid decreased rice bran enzyme activity.
- Taxon
- riceOryza sativat0
- Treatment
- acetic acide0
- Outcome
- enzyme activitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chemicals reagent s such as KI,Ag+,SDS and acetic acid decreased the enzyme activity”
t0“rice bran”
Claim · model-written summary
Ca2+ greatly increased rice bran GAD activity.
- Taxon
- riceOryza sativat0
- Treatment
- Ca2+e0
- Outcome
- GAD activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ca2+ increased the GAD activity greatly.”
t0“rice bran”
The same questions in other papers
calcium(2+) · molecular: metabolites · Oryza sativa (rice)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
silver atom · soil: biology · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.