Abstract
Viviparous1 (Vp1) plays an important role in regulating embryo development, seed dormancy, and germination in higher plants. Although we previously demonstrated a close correlation between Vp1B allelic variation and the different levels of tolerance to pre-harvest sprouting (PHS) in common wheat, little is known about the potential cis-elements in Vp1B promoter region, which may be involved in the control of PHS by Vp1B. In this study, a 2 232 bp Vp1B upstream sequence was isolated. In silico analysis revealed the presence of nine ABRE, two DREB, six MYB, three GARE, one TCA-E, two TGACG-motif, four SKn-1, and one RYREPE cis-elements in the isolated sequence. Based on this, we constructed various plasmid vectors with 5′-truncated Vp1B promoters fused with the GUS (encoding β-glucuronidase) reporter gene. Transient expression assay in wheat callus indicated that Vp1B promoter activity was inducible by low-temperature, ABA, or GA treatment. However, the level of induced GUS activity declined as promoter length decreased. Subsequently, six truncated promoter reporter fusions were introduced into the genome of durum wheat by Agrobacterium-mediated transformation and stable transgenic lines were obtained. Analysis of the transgenic lines indicated that Vp1B promoter could drive GUS expression in the anther, axis, aleurone and root, but not in the leaves, stems and nodes. However, in the plants with the transgenic Vp1B promoter fragment longer than 660 bp, GUS expression could be induced in the nodes by ABA treatment.
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Claims 9
Claim · model-written summary
A 2 232 bp Vp1B upstream sequence was isolated.
- Taxon
- Wheat
Triticum aestivumt0 - Gene
- Vp1Be0
- Outcome
- upstream sequencee0
- Value
- 2 232 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 2 232 bp Vp1B upstream sequence was isolated”
t0“Wheat”
Claim · model-written summary
In silico analysis identified nine ABRE, two DREB, six MYB, three GARE, one TCA-E, two TGACG-motif, four SKn-1, and one RYREPE elements.
- Taxon
- WheatTriticum aestivumt0
- Gene
- ABRE, two DREB, six MYB, three GARE, one TCA-E, two TGACG-motif, four SKn-1, and one RYREPEe0
- Outcome
- cis-elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In silico analysis revealed the presence of nine ABRE, two DREB, six MYB, three GARE, one TCA-E, two TGACG-motif, four SKn-1, and one RYREPE cis-elements in the isolated sequence”
t0“Wheat”
Claim · model-written summary
Low temperature induced Vp1B promoter activity in wheat callus.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- calluse0
- Treatment
- low-temperaturee0
- Gene
- Vp1Be0
- Outcome
- promoter activitye0
- Direction
- induciblee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression assay in wheat callus indicated that Vp1B promoter activity was inducible by low-temperature, ABA, or GA treatment”
Claim · model-written summary
ABA induced Vp1B promoter activity in wheat callus.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- calluse0
- Treatment
- ABAe0
- Gene
- Vp1Be0
- Outcome
- promoter activitye0
- Direction
- induciblee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression assay in wheat callus indicated that Vp1B promoter activity was inducible by low-temperature, ABA, or GA treatment”
Claim · model-written summary
GA induced Vp1B promoter activity in wheat callus.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- calluse0
- Treatment
- GAe0
- Gene
- Vp1Be0
- Outcome
- promoter activitye0
- Direction
- induciblee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression assay in wheat callus indicated that Vp1B promoter activity was inducible by low-temperature, ABA, or GA treatment”
Claim · model-written summary
Induced GUS activity declined with shorter Vp1B promoters.
- Taxon
- WheatTriticum aestivumt0
- Material
- promotere0
- Gene
- GUSe0
- Outcome
- induced GUS activitye0
- Direction
- declinede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the level of induced GUS activity declined as promoter length decreased”
t0“Wheat”
Claim · model-written summary
Vp1B promoter drove GUS expression in anther, axis, aleurone, and root.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- anther, axis, aleurone and roote0
- Gene
- Vp1Be0
- Outcome
- GUS expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vp1B promoter could drive GUS expression in the anther, axis, aleurone and root”
t0“Wheat”
Claim · model-written summary
Vp1B promoter did not drive GUS expression in leaves, stems, and nodes.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- leaves, stems and nodese0
- Gene
- Vp1Be1
- Outcome
- GUS expressione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not in the leaves, stems and nodes”
t0“Wheat”
e1“Vp1B promoter could drive GUS expression in the anther, axis, aleurone and root”
Claim · model-written summary
ABA induced nodal GUS expression in plants with a Vp1B promoter fragment longer than 660 bp.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- nodese0
- Treatment
- ABA treatmente0
- Gene
- Vp1Be0
- Outcome
- GUS expressione0
- Direction
- inducede0
- Qualifier
- longer than 660 bpe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the plants with the transgenic Vp1B promoter fragment longer than 660 bp, GUS expression could be induced in the nodes by ABA treatment”
t0“Wheat”
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