Abstract
The cold acclimation process in plants is primarily regulated through the signal transduction pathways that lead to the induction and enhancement of expression of different sets of Cor/Lea genes. Winter wheat 'Mironovskaya 808' (M808) exhibited a much higher level of freezing tolerance than spring wheat 'Chinese Spring' (CS), and the difference became clearer after the long-term cold acclimation. To understand the molecular basis of this cultivar difference, we isolated two CBF/DREB1 homologs, Wcbf2, which are the candidate gene for a transcription factor of the Cor/Lea genes. Expression of the Wcbf2 gene was induced rapidly by low temperature (LT) and drought but not by abscisic acid (ABA). The gene expression was temporal and at least twice up-regulated by LT. The first up-regulation occurred within 1-4 h, which might correspond to the rapid response to LT, while the second up-regulation occurred during 2-3 weeks of cold acclimation. After the second up-regulation, the amount of Wcbf2 transcript greatly decreased in CS, while it increased again in M808 after 4 weeks until 9 weeks (end of the test period). The maintenance of this high level of the Wcbf2 transcript might represent the long-term effect of cold acclimation. The activation of Cor/Lea genes followed the accumulation of Wcbf2 transcript suggested direct involvement of the Wcbf2 gene in the induction and enhancement of the Cor/Lea gene expression. The cultivar difference in freezing tolerance developed during different stages of cold acclimation can be at least partly explained by the differential and coordinated regulation of the predicted Cor/Lea gene signal transduction pathway that is mediated by the CBF/DREB1 transcription factors in common wheat.
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Claims 11
Claim · model-written summary
Low temperature rapidly induced Wcbf2 expression.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- low temperature (LT)e0
- Gene
- Wcbf2e0
- Outcome
- Expression of the Wcbf2 genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“two wheat cultivars”
e0“Expression of the Wcbf2 gene was induced rapidly by low temperature (LT) and drought but not by abscisic acid (ABA)”
Claim · model-written summary
Drought induced Wcbf2 expression.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- droughte0
- Gene
- Wcbf2e0
- Outcome
- Expression of the Wcbf2 genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“two wheat cultivars”
e0“Expression of the Wcbf2 gene was induced rapidly by low temperature (LT) and drought but not by abscisic acid (ABA)”
Claim · model-written summary
Abscisic acid did not induce Wcbf2 expression.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- abscisic acid (ABA)e0
- Gene
- Wcbf2e0
- Outcome
- Expression of the Wcbf2 genee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“two wheat cultivars”
e0“Expression of the Wcbf2 gene was induced rapidly by low temperature (LT) and drought but not by abscisic acid (ABA)”
Claim · model-written summary
Cold acclimation caused a second up-regulation of Wcbf2 during 2-3 weeks.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- cold acclimatione1
- Outcome
- second up-regulatione1
- Direction
- up-regulatede0
- Value
- 2-3 weekse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“two wheat cultivars”
e0“The gene expression was temporal and at least twice up-regulated by LT”
e1“while the second up-regulation occurred during 2-3 weeks of cold acclimation”
Claim · model-written summary
In Chinese Spring, the Wcbf2 transcript greatly decreased after the second up-regulation.
- Taxon
- wheatTriticum aestivumt0
- Material
- Chinese Springe1
- Gene
- Wcbf2e0
- Outcome
- amount of Wcbf2 transcripte0
- Direction
- greatly decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“two wheat cultivars”
e0“After the second up-regulation, the amount of Wcbf2 transcript greatly decreased in CS, while it increased again in M808 after 4 weeks until 9 weeks”
e1“Winter wheat 'Mironovskaya 808' (M808) exhibited a much higher level of freezing tolerance than spring wheat 'Chinese Spring' (CS)”
Claim · model-written summary
In Mironovskaya 808, the Wcbf2 transcript increased again after 4 weeks until 9 weeks of cold acclimation.
- Taxon
- wheatTriticum aestivumt0
- Material
- Mironovskaya 808e1
- Gene
- Wcbf2e0
- Outcome
- amount of Wcbf2 transcripte0
- Direction
- increased againe0
- Value
- 4 weeks until 9 weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“two wheat cultivars”
e0“After the second up-regulation, the amount of Wcbf2 transcript greatly decreased in CS, while it increased again in M808 after 4 weeks until 9 weeks”
e1“Winter wheat 'Mironovskaya 808' (M808) exhibited a much higher level of freezing tolerance than spring wheat 'Chinese Spring' (CS)”
Claim · model-written summary
Maintenance of a high level of Wcbf2 transcript might represent the long-term effect of cold acclimation.
- Taxon
- wheatTriticum aestivumt0
- Gene
- Wcbf2e0
- Outcome
- maintenance of this high level of the Wcbf2 transcripte0
- Qualifier
- long-term effect of cold acclimatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“two wheat cultivars”
e0“The maintenance of this high level of the Wcbf2 transcript might represent the long-term effect of cold acclimation”
Claim · model-written summary
Activation of Cor/Lea genes followed Wcbf2 transcript accumulation, suggesting direct involvement of Wcbf2 in Cor/Lea induction.
- Taxon
- wheatTriticum aestivumt0
- Gene
- Wcbf2e0
- Outcome
- activation of Cor/Lea genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“two wheat cultivars”
e0“The activation of Cor/Lea genes followed the accumulation of Wcbf2 transcript suggested direct involvement of the Wcbf2 gene in the induction and enhancement of the Cor/Lea gene expression”
Claim · model-written summary
The cultivar difference in freezing tolerance can be partly explained by differential and coordinated regulation of the Cor/Lea pathway mediated by CBF/DREB1 factors.
- Taxon
- common wheatTriticum aestivume0
- Gene
- CBF/DREB1e0
- Outcome
- cultivar difference in freezing tolerancee0
- Qualifier
- differential and coordinated regulation of the predicted Cor/Lea gene signal transduction pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cultivar difference in freezing tolerance developed during different stages of cold acclimation can be at least partly explained by the differential and coordinated regulation of the predicted Cor/Lea gene signal transduction pathway that is mediated by the CBF/DREB1 transcription factors in common wheat”
Claim · model-written summary
Mironovskaya 808 had a much higher freezing tolerance than Chinese Spring.
- Taxon
- wheatTriticum aestivumt0
- Material
- Mironovskaya 808e1
- Outcome
- freezing tolerancee1
- Direction
- much highere1
- Comparator
- Chinese Springe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“two wheat cultivars”
e1“Winter wheat 'Mironovskaya 808' (M808) exhibited a much higher level of freezing tolerance than spring wheat 'Chinese Spring' (CS)”
Claim · model-written summary
Two CBF/DREB1 homologs, including Wcbf2, were isolated from wheat.
- Taxon
- wheatTriticum aestivumt0
- Gene
- Wcbf2e0
- Outcome
- two CBF/DREB1 homologse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“two wheat cultivars”
e0“we isolated two CBF/DREB1 homologs, Wcbf2, which are the candidate gene for a transcription factor of the Cor/Lea genes”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Triticum aestivum (wheat)Positive and negative results
8 papers in the same direction as this paper · 34 in the opposite direction
- positive
- no effect
- positive
39 papers more in this group
See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.