Abstract
A cDNA of Phenylalanine ammonia-lyase(PAL)gene was cloned from banana(Musa spp.AAA,Williams Mutant)by using RT-PCR approach with degenerate primers based on the conserved motifs found in a number of plant PAL genes.The sequence is 1 504 bp encoding 501 amino acids.Sequence analysis showed that the amino acid sequence of PAL is high homology with other plants,especially,Jatropha curcas and Citrus reaching 93 %.By semi-quantitative PCR and physiological methods,it showed that the expression changes of stems took on disciplinarian after inoculating Fusarium oxysporum f.sp.cubense race 4(FOC4),and the expression levels more than comparison.The physiological result was identical with that of molecular biology research,meanwhile,resembling the research results of other plants.It demonstrated that the PAL enzyme played a key role in resisting banana wilt.
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Claims 6
Claim · model-written summary
Banana PAL cDNA was cloned.
Verbatim
e0“A cDNA of Phenylalanine ammonia-lyase(PAL)gene was cloned from banana(Musa spp.AAA,Williams Mutant)”
Claim · model-written summary
The PAL sequence is 1 504 bp and encodes 501 amino acids.
Verbatim
e0“The sequence is 1 504 bp encoding 501 amino acids”
t1“Phenylalanine Ammonia-lyase Gene”
t0“Banana(Musa spp. AAA,Williams Mutant)”
Claim · model-written summary
PAL amino acid homology with Jatropha curcas and Citrus reaches 93 %.
Verbatim
e0“the amino acid sequence of PAL is high homology with other plants,especially,Jatropha curcas and Citrus reaching 93 %”
t0“Banana(Musa spp. AAA,Williams Mutant)”
Claim · model-written summary
Fusarium inoculation changed banana stem PAL expression, which exceeded the comparison.
- Taxon
- bananat0
- Organ / stage
- stemse0
- Treatment
- inoculating Fusarium oxysporum f.sp.cubense race 4(FOC4)e0
- Interactor
- Fusarium oxysporum f.sp.cubense race 4(FOC4)
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume0pathogen - Gene
- PALe1
- Outcome
- expression levelse0
- Direction
- more thane0
- Comparator
- comparisone0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“the expression changes of stems took on disciplinarian after inoculating Fusarium oxysporum f.sp.cubense race 4(FOC4),and the expression levels more than comparison”
e1“A cDNA of Phenylalanine ammonia-lyase(PAL)gene was cloned from banana(Musa spp.AAA,Williams Mutant)”
t0“Banana(Musa spp. AAA,Williams Mutant)”
Claim · model-written summary
Physiological results agreed with the molecular findings.
Verbatim
e0“The physiological result was identical with that of molecular biology research”
t0“Banana(Musa spp. AAA,Williams Mutant)”
Claim · model-written summary
The authors infer PAL enzyme plays a key role in banana wilt resistance.
Verbatim
e0“It demonstrated that the PAL enzyme played a key role in resisting banana wilt”
t0“Banana(Musa spp. AAA,Williams Mutant)”
The same questions in other papers
inoculation · molecular: expression · MusaPositive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.