Abstract
Seeking monoclone in established cDNA library randomly for PCR directed with universal M13 primer and sequencing,comparing the sequence in GenBank,designing specific primer according to the sequence,finally we obtained the whole ORF of siCOR.Then plant expression vector pBIN438-siCOR was constructed and NC89 tobacco plants were transformed via Agrobacterium tumefaciens,RT-PCR analysis indicated that siCOR had been integrated into the genome.Homozygous transgenic tobacco plants were used in cold resistance identification.The results show that:(1)The size of siCOR cDNA was 661 bp,the ideutity of amino acid sequence was about 65.04% with Oryza sativa Japonica.(2)siCOR tobacco has deep green leaves,grows faster and has no lateral bud compared with wild type;Under the cold stress,the decrease of relative water content,reduction of relative quantum yield of PSⅡ,the increase of relative conductivity and MDA content of siCOR tobacco are lesser than that of control tobacco evidently.Besides,transgenic plants have slower and lighter symptom.In summary,under the cold stress,siCOR tobacco shows well growth traits and strong cold resistance characteristics.
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Claims 6
Claim · model-written summary
The siCOR cDNA was 661 bp long and had about 65.04% amino acid identity with Oryza sativa Japonica.
- Taxon
- Saussurea involucrataSaussurea involucratat0
- Gene
- siCORe0
- Outcome
- size of siCOR cDNAe0
- Value
- 661 bpe0
Verbatim
e0“The size of siCOR cDNA was 661 bp,the ideutity of amino acid sequence was about 65.04% with Oryza sativa Japonica.”
t0“Saussurea involucrata”
Claim · model-written summary
RT-PCR indicated integration of siCOR into tobacco genome.
- Taxon
- tobacco plants
Nicotiana tabacumt0 - Gene
- siCORe0
- Outcome
- siCOR had been integrated into the genomee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RT-PCR analysis indicated that siCOR had been integrated into the genome.”
t0“Tobacco Plants”
Claim · model-written summary
siCOR tobacco had deep green leaves, faster growth and no lateral buds versus wild type.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- siCORe0
- Outcome
- deep green leavese0
- Comparator
- wild typee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“siCOR tobacco has deep green leaves,grows faster and has no lateral bud compared with wild type”
Claim · model-written summary
Under cold stress, siCOR tobacco had smaller declines in relative water content and PSII quantum yield than controls.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- siCORe0
- Outcome
- relative water contente0
- Direction
- lessere0
- Qualifier
- cold stresse0
- Comparator
- control tobaccoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under the cold stress,the decrease of relative water content,reduction of relative quantum yield of PSⅡ,the increase of relative conductivity and MDA content of siCOR tobacco are lesser than that of control tobacco evidently.”
Claim · model-written summary
Under cold stress, siCOR tobacco had smaller increases in conductivity and MDA content than controls.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- siCORe0
- Outcome
- relative conductivity and MDA contente0
- Direction
- lessere0
- Qualifier
- cold stresse0
- Comparator
- control tobaccoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under the cold stress,the decrease of relative water content,reduction of relative quantum yield of PSⅡ,the increase of relative conductivity and MDA content of siCOR tobacco are lesser than that of control tobacco evidently.”
Claim · model-written summary
Transgenic tobacco developed slower and lighter cold-stress symptoms.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- transgenic plantse0
- Outcome
- symptome0
- Direction
- slower and lightere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic plants have slower and lighter symptom”
t0“Tobacco Plants”