Abstract
Using barley(Hordeum vulgare) variety 'Hua 30' as donor material,effects on callus yield under 300mg·L-1 NaCl stress and the green plantlet differentiated rate under 0.3% NaCl stress from the microspores treated with ethyl methane sulfonate(EMS) and bleomycin or the microspores from irradiated spikes and dry seeds were investigated.The results showed that callus yield and green plantlet from microspores with EMS treatment and microspore derived from irradiated dry seeds were obviously superior to the microspores treated by bleomycin and microspore derived from irradiated spikes.A series of indexes such as seed germination rate,seedling survival rate,plant tiller number,plant height and yield per plant were compared on seeds from 16 regenerated plants by seed irradiation under 0.3% NaCl stress.The germination rate of 'Hua 30' is 0,while that of 14 of the 16 salt tolerance mutants has an advantage over 'Hua 30',moreover,4 materials were obviously better than 'Hua 30.Based on these salt tolerant mutants,the expression patterns and quantity of Na+/H+ antiporter genes(NHX1,NHX2 and NHX3),and BBD1 and BBD2 encoding two betaine aldehyde dehydrogenase(BADH) isozymes were measured.As a result,the improvement in salt tolerance of variants had corresponding relation with these genes.
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Claims 4
Claim · model-written summary
EMS treated microspores gave superior callus yield and green plantlets relative to bleomycin treated microspores.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- EMS treatmente0
- Outcome
- callus yield and green plantlete0
- Direction
- superiore0
- Comparator
- microspores treated by bleomycine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“callus yield and green plantlet from microspores with EMS treatment and microspore derived from irradiated dry seeds were obviously superior to the microspores treated by bleomycin and microspore derived from irradiated spikes”
t0“Barley(Hordeum vulgare L.)”
Claim · model-written summary
Microspores from irradiated dry seeds gave superior callus yield and green plantlets to those from irradiated spikes.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- irradiated dry seedse0
- Outcome
- callus yield and green plantlete0
- Direction
- superiore0
- Comparator
- irradiated spikese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus yield and green plantlet from microspores with EMS treatment and microspore derived from irradiated dry seeds were obviously superior to the microspores treated by bleomycin and microspore derived from irradiated spikes”
t0“Barley(Hordeum vulgare L.)”
Claim · model-written summary
Fourteen of sixteen mutants had higher germination than Hua 30 under salt stress, with four markedly better.
- Taxon
- barleyHordeum vulgaret0
- Material
- salt tolerance mutantse0
- Outcome
- germination ratee0
- Value
- 14 of the 16e0
- Comparator
- 'Hua 30'e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The germination rate of 'Hua 30' is 0,while that of 14 of the 16 salt tolerance mutants has an advantage over 'Hua 30',moreover,4 materials were obviously better than 'Hua 30”
t0“Barley(Hordeum vulgare L.)”
Claim · model-written summary
Improved salt tolerance in variants corresponded to expression of NHX and BBD genes.
- Taxon
- barleyHordeum vulgaret0
- Gene
- these genese0
- Outcome
- improvement in salt tolerancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the improvement in salt tolerance of variants had corresponding relation with these genes”
t0“Barley(Hordeum vulgare L.)”
The same questions in other papers
radiation · yield · Hordeum vulgare (barley)Positive and negative results
8 papers in the same direction as this paper · 3 in the opposite direction
- negative
- no effect
- negative
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.